Evidence Source

Description	fibroblast cell culture secretome comparison of treatment with / without recombinant MMP-2 incubation Termini reported show a ration between 0.75 and 1.5 after quantification and are therefore considered not to be affected by the protease.
Method	N-TAILS
Experimental system	cell culture
Perturbation
Protease inhibitor(s) used
Directness	direct
Physiological relevance	likely
Evidence Code(s)
Database / Laboratory	- Lab: Overall CM.
Raw data repository

Evidence for:

Publication(s)	Isotopic labeling of terminal amines in complex samples identifies protein N-termini and protease cleavage products. Kleifeld O, Doucet A, auf dem Keller U, Prudova A, Schilling O, Kainthan RK, Starr AE, Foster LJ, Kizhakkedathu JN, Overall CM Nat Biotechnol. 2010 Mar;28(3):281-8. Epub 2010 Mar 7. PMID: 20208520

Publication(s)

Isotopic labeling of terminal amines in complex samples identifies protein N-termini and protease cleavage products.

Kleifeld O, Doucet A, auf dem Keller U, Prudova A, Schilling O, Kainthan RK, Starr AE, Foster LJ, Kizhakkedathu JN, Overall CM