Evidence Source

Description	Inferred from cleavage recMMP-Chemokine_AStarr2012A: In vitro chemokine cleavage assays with recombinant MMPs were performed in 50 mM Tris, 200 mM NaCl, 5 mM CaCl2, pH 7.4 for 16 h at 37 Ã‚Â°C. Initially, an enzyme to substrate molar ratio of 1:10 was assessed to screen for cleavage activity, followed by lower enzyme concentrations to 1:1,000 to suggest biological relevance. Cleavage assay products were analyzed, as above, by MALDI-TOF MS and confirmed by silver-staining following 15% Tris-Tricine SDS- PAGE. Chemokine cleavage was defined to be positive when the MS spectra showed a cleavage product with greater than 20% ion intensity of the full-length chemokine.
Method	electronic annotation
Experimental system	unknown
Perturbation	none
Protease inhibitor(s) used
Directness	indirect
Physiological relevance	unknown
Evidence Code(s)	inferred from cleavage
Database / Laboratory	- DB: TopFIND
Raw data repository

Evidence for:

Publication(s)