Evidence Source

Description	Identification of protein N termini from human erythrocytes with and without hemoglobin depletion. Proteins and protein N termini were isolated and enriched from human erythrocytes following the TAILS procedure with heavy dimethyl labeling of free N-termini (Kleifeld et al. Nat Biothech 2010). Following LC-MS/MS analysis on a Thermo Orbitrap XL spectra were matched using Mascot, X!Tandem and MSGF+ databases search engines and trans proteomic pipeline (TPP) modules for statistical evaluation. Maximum peptide level FDR was set to 1% (iProphet probability 0.88) and protein level FDR to 0.7% (proteinProphet probability 0.9).
Method
Experimental system	in vivo sample
Perturbation	none
Protease inhibitor(s) used	10 mM Hepes, 10 mM EDTA, 1 mM PMSF, 5x HALT! Protease Inhibitor Cocktail
Directness	direct
Physiological relevance	yes
Evidence Code(s)
Database / Laboratory	- Lab: Overall Laboratory
Raw data repository	http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD000434

Evidence for:

Publication(s)