Q14674: Separin

General Information

Protein names	- Separin - 3.4.22.49 - Caspase-like protein ESPL1 - Extra spindle poles-like 1 protein - Separase
Gene names	ESPL1
Organism	Homo sapiens
Protease Family	C50.002
Protease ID	C50.002
Chromosome location
UniProt ID	Q14674

5 N-termini

4 C-termini

3 Cleavages

6 Substrates

Sequence

        10         20         30         40         50         60
MRSFKRVNFG TLLSSQKEAE ELLPALKEFL SNPPAGFPSS RSDAERRQAC DAILRACNQQ
        70         80         90        100        110        120
LTAKLACPRH LGSLLELAEL ACDGYLVSTP QRPPLYLERI LFVLLRNAAA QGSPEATLRL
       130        140        150        160        170        180
AQPLHACLVQ CSREAAPQDY EAVARGSFSL LWKGAEALLE RRAAFAARLK ALSFLVLLED
       190        200        210        220        230        240
ESTPCEVPHF ASPTACRAVA AHQLFDASGH GLNEADADFL DDLLSRHVIR ALVGERGSSS
       250        260        270        280        290        300
GLLSPQRALC LLELTLEHCR RFCWSRHHDK AISAVEKAHS YLRNTNLAPS LQLCQLGVKL
       310        320        330        340        350        360
LQVGEEGPQA VAKLLIKASA VLSKSMEAPS PPLRALYESC QFFLSGLERG TKRRYRLDAI
       370        380        390        400        410        420
LSLFAFLGGY CSLLQQLRDD GVYGGSSKQQ QSFLQMYFQG LHLYTVVVYD FAQGCQIVDL
       430        440        450        460        470        480
ADLTQLVDSC KSTVVWMLEA LEGLSGQELT DHMGMTASYT SNLAYSFYSH KLYAEACAIS
       490        500        510        520        530        540
EPLCQHLGLV KPGTYPEVPP EKLHRCFRLQ VESLKKLGKQ AQGCKMVILW LAALQPCSPE
       550        560        570        580        590        600
HMAEPVTFWV RVKMDAARAG DKELQLKTLR DSLSGWDPET LALLLREELQ AYKAVRADTG
       610        620        630        640        650        660
QERFNIICDL LELSPEETPA GAWARATHLV ELAQVLCYHD FTQQTNCSAL DAIREALQLL
       670        680        690        700        710        720
DSVRPEAQAR DQLLDDKAQA LLWLYICTLE AKMQEGIERD RRAQAPGNLE EFEVNDLNYE
       730        740        750        760        770        780
DKLQEDRFLY SNIAFNLAAD AAQSKCLDQA LALWKELLTK GQAPAVRCLQ QTAASLQILA
       790        800        810        820        830        840
ALYQLVAKPM QALEVLLLLR IVSERLKDHS KAAGSSCHIT QLLLTLGCPS YAQLHLEEAA
       850        860        870        880        890        900
SSLKHLDQTT DTYLLLSLTC DLLRSQLYWT HQKVTKGVSL LLSVLRDPAL QKSSKAWYLL
       910        920        930        940        950        960
RVQVLQLVAA YLSLPSNNLS HSLWEQLCAQ GWQTPEIALI DSHKLLRSII LLLMGSDILS
       970        980        990       1000       1010       1020
TQKAAVETSF LDYGENLVQK WQVLSEVLSC SEKLVCHLGR LGSVSEAKAF CLEALKLTTK
      1030       1040       1050       1060       1070       1080
LQIPRQCALF LVLKGELELA RNDIDLCQSD LQQVLFLLES CTEFGGVTQH LDSVKKVHLQ
      1090       1100       1110       1120       1130       1140
KGKQQAQVPC PPQLPEEELF LRGPALELVA TVAKEPGPIA PSTNSSPVLK TKPQPIPNFL
      1150       1160       1170       1180       1190       1200
SHSPTCDCSL CASPVLTAVC LRWVLVTAGV RLAMGHQAQG LDLLQVVLKG CPEAAERLTQ
      1210       1220       1230       1240       1250       1260
ALQASLNHKT PPSLVPSLLD EILAQAYTLL ALEGLNQPSN ESLQKVLQSG LKFVAARIPH
      1270       1280       1290       1300       1310       1320
LEPWRASLLL IWALTKLGGL SCCTTQLFAS SWGWQPPLIK SVPGSEPSKT QGQKRSGRGR
      1330       1340       1350       1360       1370       1380
QKLASAPLRL NNTSQKGLEG RGLPCTPKPP DRIRQAGPHV PFTVFEEVCP TESKPEVPQA
      1390       1400       1410       1420       1430       1440
PRVQQRVQTR LKVNFSDDSD LEDPVSAEAW LAEEPKRRGT ASRGRGRARK GLSLKTDAVV
      1450       1460       1470       1480       1490       1500
APGSAPGNPG LNGRSRRAKK VASRHCEERR PQRASDQARP GPEIMRTIPE EELTDNWRKM
      1510       1520       1530       1540       1550       1560
SFEILRGSDG EDSASGGKTP APGPEAASGE WELLRLDSSK KKLPSPCPDK ESDKDLGPRL
      1570       1580       1590       1600       1610       1620
RLPSAPVATG LSTLDSICDS LSVAFRGISH CPPSGLYAHL CRFLALCLGH RDPYATAFLV
      1630       1640       1650       1660       1670       1680
TESVSITCRH QLLTHLHRQL SKAQKHRGSL EIADQLQGLS LQEMPGDVPL ARIQRLFSFR
      1690       1700       1710       1720       1730       1740
ALESGHFPQP EKESFQERLA LIPSGVTVCV LALATLQPGT VGNTLLLTRL EKDSPPVSVQ
      1750       1760       1770       1780       1790       1800
IPTGQNKLHL RSVLNEFDAI QKAQKENSSC TDKREWWTGR LALDHRMEVL IASLEKSVLG
      1810       1820       1830       1840       1850       1860
CWKGLLLPSS EEPGPAQEAS RLQELLQDCG WKYPDRTLLK IMLSGAGALT PQDIQALAYG
      1870       1880       1890       1900       1910       1920
LCPTQPERAQ ELLNEAVGRL QGLTVPSNSH LVLVLDKDLQ KLPWESMPSL QALPVTRLPS
      1930       1940       1950       1960       1970       1980
FRFLLSYSII KEYGASPVLS QGVDPRSTFY VLNPHNNLSS TEEQFRANFS SEAGWRGVVG
      1990       2000       2010       2020       2030       2040
EVPRPEQVQE ALTKHDLYIY AGHGAGARFL DGQAVLRLSC RAVALLFGCS SAALAVRGNL
      2050       2060       2070       2080       2090       2100
EGAGIVLKYI MAGCPLFLGN LWDVTDRDID RYTEALLQGW LGAGPGAPLL YYVNQARQAP
      2110       2120
RLKYLIGAAP IAYGLPVSLR

Isoforms

- Isoform 2 of Separin

Sequence View

        10         20         30         40         50         60
MRSFKRVNFG TLLSSQKEAE ELLPALKEFL SNPPAGFPSS RSDAERRQAC DAILRACNQQ
        70         80         90        100        110        120
LTAKLACPRH LGSLLELAEL ACDGYLVSTP QRPPLYLERI LFVLLRNAAA QGSPEATLRL
       130        140        150        160        170        180
AQPLHACLVQ CSREAAPQDY EAVARGSFSL LWKGAEALLE RRAAFAARLK ALSFLVLLED
       190        200        210        220        230        240
ESTPCEVPHF ASPTACRAVA AHQLFDASGH GLNEADADFL DDLLSRHVIR ALVGERGSSS
       250        260        270        280        290        300
GLLSPQRALC LLELTLEHCR RFCWSRHHDK AISAVEKAHS YLRNTNLAPS LQLCQLGVKL
       310        320        330        340        350        360
LQVGEEGPQA VAKLLIKASA VLSKSMEAPS PPLRALYESC QFFLSGLERG TKRRYRLDAI
       370        380        390        400        410        420
LSLFAFLGGY CSLLQQLRDD GVYGGSSKQQ QSFLQMYFQG LHLYTVVVYD FAQGCQIVDL
       430        440        450        460        470        480
ADLTQLVDSC KSTVVWMLEA LEGLSGQELT DHMGMTASYT SNLAYSFYSH KLYAEACAIS
       490        500        510        520        530        540
EPLCQHLGLV KPGTYPEVPP EKLHRCFRLQ VESLKKLGKQ AQGCKMVILW LAALQPCSPE
       550        560        570        580        590        600
HMAEPVTFWV RVKMDAARAG DKELQLKTLR DSLSGWDPET LALLLREELQ AYKAVRADTG
       610        620        630        640        650        660
QERFNIICDL LELSPEETPA GAWARATHLV ELAQVLCYHD FTQQTNCSAL DAIREALQLL
       670        680        690        700        710        720
DSVRPEAQAR DQLLDDKAQA LLWLYICTLE AKMQEGIERD RRAQAPGNLE EFEVNDLNYE
       730        740        750        760        770        780
DKLQEDRFLY SNIAFNLAAD AAQSKCLDQA LALWKELLTK GQAPAVRCLQ QTAASLQILA
       790        800        810        820        830        840
ALYQLVAKPM QALEVLLLLR IVSERLKDHS KAAGSSCHIT QLLLTLGCPS YAQLHLEEAA
       850        860        870        880        890        900
SSLKHLDQTT DTYLLLSLTC DLLRSQLYWT HQKVTKGVSL LLSVLRDPAL QKSSKAWYLL
       910        920        930        940        950        960
RVQVLQLVAA YLSLPSNNLS HSLWEQLCAQ GWQTPEIALI DSHKLLRSII LLLMGSDILS
       970        980        990       1000       1010       1020
TQKAAVETSF LDYGENLVQK WQVLSEVLSC SEKLVCHLGR LGSVSEAKAF CLEALKLTTK
      1030       1040       1050       1060       1070       1080
LQIPRQCALF LVLKGELELA RNDIDLCQSD LQQVLFLLES CTEFGGVTQH LDSVKKVHLQ
      1090       1100       1110       1120       1130       1140
KGKQQAQVPC PPQLPEEELF LRGPALELVA TVAKEPGPIA PSTNSSPVLK TKPQPIPNFL
      1150       1160       1170       1180       1190       1200
SHSPTCDCSL CASPVLTAVC LRWVLVTAGV RLAMGHQAQG LDLLQVVLKG CPEAAERLTQ
      1210       1220       1230       1240       1250       1260
ALQASLNHKT PPSLVPSLLD EILAQAYTLL ALEGLNQPSN ESLQKVLQSG LKFVAARIPH
      1270       1280       1290       1300       1310       1320
LEPWRASLLL IWALTKLGGL SCCTTQLFAS SWGWQPPLIK SVPGSEPSKT QGQKRSGRGR
      1330       1340       1350       1360       1370       1380
QKLASAPLRL NNTSQKGLEG RGLPCTPKPP DRIRQAGPHV PFTVFEEVCP TESKPEVPQA
      1390       1400       1410       1420       1430       1440
PRVQQRVQTR LKVNFSDDSD LEDPVSAEAW LAEEPKRRGT ASRGRGRARK GLSLKTDAVV
      1450       1460       1470       1480       1490       1500
APGSAPGNPG LNGRSRRAKK VASRHCEERR PQRASDQARP GPEIMRTIPE EELTDNWRKM
      1510       1520       1530       1540       1550       1560
SFEILRGSDG EDSASGGKTP APGPEAASGE WELLRLDSSK KKLPSPCPDK ESDKDLGPRL
      1570       1580       1590       1600       1610       1620
RLPSAPVATG LSTLDSICDS LSVAFRGISH CPPSGLYAHL CRFLALCLGH RDPYATAFLV
      1630       1640       1650       1660       1670       1680
TESVSITCRH QLLTHLHRQL SKAQKHRGSL EIADQLQGLS LQEMPGDVPL ARIQRLFSFR
      1690       1700       1710       1720       1730       1740
ALESGHFPQP EKESFQERLA LIPSGVTVCV LALATLQPGT VGNTLLLTRL EKDSPPVSVQ
      1750       1760       1770       1780       1790       1800
IPTGQNKLHL RSVLNEFDAI QKAQKENSSC TDKREWWTGR LALDHRMEVL IASLEKSVLG
      1810       1820       1830       1840       1850       1860
CWKGLLLPSS EEPGPAQEAS RLQELLQDCG WKYPDRTLLK IMLSGAGALT PQDIQALAYG
      1870       1880       1890       1900       1910       1920
LCPTQPERAQ ELLNEAVGRL QGLTVPSNSH LVLVLDKDLQ KLPWESMPSL QALPVTRLPS
      1930       1940       1950       1960       1970       1980
FRFLLSYSII KEYGASPVLS QGVDPRSTFY VLNPHNNLSS TEEQFRANFS SEAGWRGVVG
      1990       2000       2010       2020       2030       2040
EVPRPEQVQE ALTKHDLYIY AGHGAGARFL DGQAVLRLSC RAVALLFGCS SAALAVRGNL
      2050       2060       2070       2080       2090       2100
EGAGIVLKYI MAGCPLFLGN LWDVTDRDID RYTEALLQGW LGAGPGAPLL YYVNQARQAP
      2110       2120
RLKYLIGAAP IAYGLPVSLR

Name	Sequence	Position	Evidence type	Method	Source (database)	Evidence name
Q14674-1-unknown	MRSFKR...	1	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
Q14674-326-unknown	MEAPSP...	326	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt74191
Q14674-1487-unknown	TIPEEE...	1487	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9584
Q14674-1487-unknown	TIPEEE...	1487	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt157520
Q14674-1507-unknown	GSDGED...	1507	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9586
Q14674-1507-unknown	GSDGED...	1507	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt157630
Q14674-1536-unknown	LDSSKK...	1536	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9588
Q14674-1536-unknown	LDSSKK...	1536	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt157715

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...PEIMRT	1486	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9584
...PEIMRT	1486	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt141019
...FEILRG	1506	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9586
...FEILRG	1506	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt141129
...WELLRL	1535	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9588
...WELLRL	1535	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt141214
...PVSLR	2120	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...PVSLR	2120	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt69809

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
ESPL1_HUMAN	1486	IMRT.\|.TIPE	inferred from experiment	unknown	MEROPS	Pellman D	Chestukhin A et al.:Processing, localization, and r... (C50.002)	12672959,
ESPL1_HUMAN	1506	ILRG.\|.GSDG	inferred from experiment	unknown	MEROPS	Pellman D	Chestukhin A et al.:Processing, localization, and r... (C50.002)	12672959,
ESPL1_HUMAN	1535	LLRL.\|.LDSS	inferred from experiment	unknown	MEROPS	Pellman D	Chestukhin A et al.:Processing, localization, and r... (C50.002)	12672959,

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
ESPL1-Isoform2_HUMAN	1161	IMRT.\|.TIPE	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC9584
ESPL1-Isoform2_HUMAN	1181	ILRG.\|.GSDG	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC9586
ESPL1-Isoform2_HUMAN	1210	LLRL.\|.LDSS	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TC9588
ESPL1_HUMAN	1486	IMRT.\|.TIPE	inferred from experiment	unknown	MEROPS	Pellman D	Chestukhin A et al.:Processing, localization, and r... (C50.002)	12672959,
ESPL1_HUMAN	1506	ILRG.\|.GSDG	inferred from experiment	unknown	MEROPS	Pellman D	Chestukhin A et al.:Processing, localization, and r... (C50.002)	12672959,
ESPL1_HUMAN	1535	LLRL.\|.LDSS	inferred from experiment	unknown	MEROPS	Pellman D	Chestukhin A et al.:Processing, localization, and r... (C50.002)	12672959,

TopFIND

Q14674: Separin

General Information

5

N-termini

4

C-termini

3

Cleavages

6

Substrates

Sequence

Isoforms

Filter Information:

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Evidence Codes:

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Protein Neighborhood

Domains & Features

5 N-termini - 4 C-termini - 3 Cleavages - 6 Substrates

N-termini

C-termini

Cleavages

Substrates