Q96IV0: Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

General Information

Protein names	- Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase - PNGase - hPNGase - 3.5.1.52 - N-glycanase 1 - Peptide:N-glycanase
Gene names	NGLY1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	Q96IV0

6 N-termini

2 C-termini

0 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform 2 of Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase - Isoform 3 of Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase - Isoform 4 of Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase - Isoform 5 of Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

Sequence View

        10         20         30         40         50         60
MAAAALGSSS GSASPAVAEL CQNTPETFLE ASKLLLTYAD NILRNPNDEK YRSIRIGNTA
        70         80         90        100        110        120
FSTRLLPVRG AVECLFEMGF EEGETHLIFP KKASVEQLQK IRDLIAIERS SRLDGSNKSH
       130        140        150        160        170        180
KVKSSQQPAA STQLPTTPSS NPSGLNQHTR NRQGQSSDPP SASTVAADSA ILEVLQSNIQ
       190        200        210        220        230        240
HVLVYENPAL QEKALACIPV QELKRKSQEK LSRARKLDKG INISDEDFLL LELLHWFKEE
       250        260        270        280        290        300
FFHWVNNVLC SKCGGQTRSR DRSLLPSDDE LKWGAKEVED HYCDACQFSN RFPRYNNPEK
       310        320        330        340        350        360
LLETRCGRCG EWANCFTLCC RAVGFEARYV WDYTDHVWTE VYSPSQQRWL HCDACEDVCD
       370        380        390        400        410        420
KPLLYEIGWG KKLSYVIAFS KDEVVDVTWR YSCKHEEVIA RRTKVKEALL RDTINGLNKQ
       430        440        450        460        470        480
RQLFLSENRR KELLQRIIVE LVEFISPKTP KPGELGGRIS GSVAWRVARG EMGLQRKETL
       490        500        510        520        530        540
FIPCENEKIS KQLHLCYNIV KDRYVRVSNN NQTISGWENG VWKMESIFRK VETDWHMVYL
       550        560        570        580        590        600
ARKEGSSFAY ISWKFECGSV GLKVDSISIR TSSQTFQTGT VEWKLRSDTA QVELTGDNSL
       610        620        630        640        650
HSYADFSGAT EVILEAELSR GDGDVAWQHT QLFRQSLNDH EENCLEIIIK FSDL         10         20         30         40         50         60
MAAAALGSSS GSASPAVAEL CQNTPETFLE ASKLLLTYAD NILRNPNDEK YRSIRIGNTA
        70         80         90        100        110        120
FSTRLLPVRG AVECLFEMGF EEGETHLIFP KKASVEQLQK IRDLIAIERS SRLDGSNKSH
       130        140        150        160        170        180
KVKSSQQPAA STQLPTTPSS NPSGLNQHTR NRQGQSSDPP SASTVAADSA ILEVLQSNIQ
       190        200        210        220        230        240
HVLVYENPAL QEKALACIPV QELKRKSQEK LSRARKLDKG INISDEDFLL LELLHWFKEE
       250        260        270        280        290        300
FFHWVNNVLC SKCGGQTRSR DRSLLPSDDE LKWGAKEVED HYCDACQFSN RFPRYNNPEK
       310        320        330        340        350        360
LLETRCGRCG EWANCFTLCC RAVGFEARYV WDYTDHVWTE VYSPSQQRWL HCDACEDVCD
       370        380        390        400        410        420
KPLLYEIGWG KKLSYVIAFS KDEVVDVTWR YSCKHEEVIA RRTKVKEALL RDTINGLNKQ
       430        440        450        460        470        480
RQLFLSENRR KELLQRIIVE LVEFISPKTP KPGELGGRIS GSVAWRVARG EMGLQRKETL
       490        500        510        520        530        540
FIPCENEKIS KQLHLCYNIV KDRYVRVSNN NQTISGWENG VWKMESIFRK VETDWHMVYL
       550        560        570        580        590        600
ARKEGSSFAY ISWKFECGSV GLKVDSISIR TSSQTFQTGT VEWKLRSDTA QVELTGDNSL
       610        620        630        640        650
HSYADFSGAT EVILEAELSR GDGDVAWQHT QLFRQSLNDH EENCLEIIIK FSDL         10         20         30         40         50         60
MAAAALGSSS GSASPAVAEL CQNTPETFLE ASKLLLTYAD NILRNPNDEK YRSIRIGNTA
        70         80         90        100        110        120
FSTRLLPVRG AVECLFEMGF EEGETHLIFP KKASVEQLQK IRDLIAIERS SRLDGSNKSH
       130        140        150        160        170        180
KVKSSQQPAA STQLPTTPSS NPSGLNQHTR NRQGQSSDPP SASTVAADSA ILEVLQSNIQ
       190        200        210        220        230        240
HVLVYENPAL QEKALACIPV QELKRKSQEK LSRARKLDKG INISDEDFLL LELLHWFKEE
       250        260        270        280        290        300
FFHWVNNVLC SKCGGQTRSR DRSLLPSDDE LKWGAKEVED HYCDACQFSN RFPRYNNPEK
       310        320        330        340        350        360
LLETRCGRCG EWANCFTLCC RAVGFEARYV WDYTDHVWTE VYSPSQQRWL HCDACEDVCD
       370        380        390        400        410        420
KPLLYEIGWG KKLSYVIAFS KDEVVDVTWR YSCKHEEVIA RRTKVKEALL RDTINGLNKQ
       430        440        450        460        470        480
RQLFLSENRR KELLQRIIVE LVEFISPKTP KPGELGGRIS GSVAWRVARG EMGLQRKETL
       490        500        510        520        530        540
FIPCENEKIS KQLHLCYNIV KDRYVRVSNN NQTISGWENG VWKMESIFRK VETDWHMVYL
       550        560        570        580        590        600
ARKEGSSFAY ISWKFECGSV GLKVDSISIR TSSQTFQTGT VEWKLRSDTA QVELTGDNSL
       610        620        630        640        650
HSYADFSGAT EVILEAELSR GDGDVAWQHT QLFRQSLNDH EENCLEIIIK FSDL         10         20         30         40         50         60
MAAAALGSSS GSASPAVAEL CQNTPETFLE ASKLLLTYAD NILRNPNDEK YRSIRIGNTA
        70         80         90        100        110        120
FSTRLLPVRG AVECLFEMGF EEGETHLIFP KKASVEQLQK IRDLIAIERS SRLDGSNKSH
       130        140        150        160        170        180
KVKSSQQPAA STQLPTTPSS NPSGLNQHTR NRQGQSSDPP SASTVAADSA ILEVLQSNIQ
       190        200        210        220        230        240
HVLVYENPAL QEKALACIPV QELKRKSQEK LSRARKLDKG INISDEDFLL LELLHWFKEE
       250        260        270        280        290        300
FFHWVNNVLC SKCGGQTRSR DRSLLPSDDE LKWGAKEVED HYCDACQFSN RFPRYNNPEK
       310        320        330        340        350        360
LLETRCGRCG EWANCFTLCC RAVGFEARYV WDYTDHVWTE VYSPSQQRWL HCDACEDVCD
       370        380        390        400        410        420
KPLLYEIGWG KKLSYVIAFS KDEVVDVTWR YSCKHEEVIA RRTKVKEALL RDTINGLNKQ
       430        440        450        460        470        480
RQLFLSENRR KELLQRIIVE LVEFISPKTP KPGELGGRIS GSVAWRVARG EMGLQRKETL
       490        500        510        520        530        540
FIPCENEKIS KQLHLCYNIV KDRYVRVSNN NQTISGWENG VWKMESIFRK VETDWHMVYL
       550        560        570        580        590        600
ARKEGSSFAY ISWKFECGSV GLKVDSISIR TSSQTFQTGT VEWKLRSDTA QVELTGDNSL
       610        620        630        640        650
HSYADFSGAT EVILEAELSR GDGDVAWQHT QLFRQSLNDH EENCLEIIIK FSDL

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

6 N-termini - 2 C-termini - 0 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Evidence name
Q96IV0-2-unknown	MAAAAL...	1		inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
Q96IV0-1-unknown	MAAAAL...	1		inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt82818
Q96IV0-1-unknown	MAAAAL...	1		inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt82819
Q96IV0-2-Acetylation	AAAALG...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
Q96IV0-2-Acetylation	AAAALG...	2	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt110147
Q96IV0-2-Acetylation	AAAALG...	2	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt110148
Q96IV0-4-unknown	AALGSS...	4		inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000307980
Q96IV0-4-unknown	AALGSS...	4		inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt194616
Q96IV0-4-unknown	AALGSS...	4		inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt194617
Q96IV0-78-unknown	MGFEEG...	78		inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000412946
Q96IV0-78-unknown	MGFEEG...	78		inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000395878
Q96IV0-78-unknown	MGFEEG...	78		inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt196616
Q96IV0-78-unknown	MGFEEG...	78		inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt196617
Q96IV0-78-unknown	MGFEEG...	78		inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt196618
Q96IV0-524-unknown	MESIFR...	524		inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt82820
Q96IV0-524-unknown	MESIFR...	524		inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt103379
Q96IV0-524-unknown	MESIFR...	524		inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TNt103380

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...NPALQE	191	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000412946
...NPALQE	191	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt155530
...NPALQE	191	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt155531
...NPALQE	191	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt155532
...KFSDL	654	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...KFSDL	654	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt78438
...KFSDL	654	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt78439
...KFSDL	654	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt78436

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)