O14727: Apoptotic protease-activating factor 1 {ECO:0000305}

General Information

Protein names	- Apoptotic protease-activating factor 1 {ECO:0000305} - APAF-1
Gene names	APAF1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	O14727

3 N-termini

2 C-termini

2 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform 2 of Apoptotic protease-activating factor 1 - Isoform 3 of Apoptotic protease-activating factor 1 - Isoform 4 of Apoptotic protease-activating factor 1 - Isoform 5 of Apoptotic protease-activating factor 1 - Isoform 6 of Apoptotic protease-activating factor 1

Sequence View

        10         20         30         40         50         60
MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ QRAAMLIKMI
        70         80         90        100        110        120
LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK DSVSGITSYV RTVLCEGGVP
       130        140        150        160        170        180
QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG
       190        200        210        220        230        240
GVHWVSVGKQ DKSGLLMKLQ NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL
       250        260        270        280        290        300
ILDDVWDSWV LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN
       310        320        330        340        350        360
MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF KRIRKSSSYD
       370        380        390        400        410        420
YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL CILWDMETEE VEDILQEFVN
       430        440        450        460        470        480
KSLLFCDRNG KSFRYYLHDL QVDFLTEKNC SQLQDLHKKI ITQFQRYHQP HTLSPDQEDC
       490        500        510        520        530        540
MYWYNFLAYH MASAKMHKEL CALMFSLDWI KAKTELVGPA HLIHEFVEYR HILDEKDCAV
       550        560        570        580        590        600
SENFQEFLSL NGHLLGRQPF PNIVQLGLCE PETSEVYQQA KLQAKQEVDN GMLYLEWINK
       610        620        630        640        650        660
KNITNLSRLV VRPHTDAVYH ACFSEDGQRI ASCGADKTLQ VFKAETGEKL LEIKAHEDEV
       670        680        690        700        710        720
LCCAFSTDDR FIATCSVDKK VKIWNSMTGE LVHTYDEHSE QVNCCHFTNS SHHLLLATGS
       730        740        750        760        770        780
SDCFLKLWDL NQKECRNTMF GHTNSVNHCR FSPDDKLLAS CSADGTLKLW DATSANERKS
       790        800        810        820        830        840
INVKQFFLNL EDPQEDMEVI VKCCSWSADG ARIMVAAKNK IFLFDIHTSG LLGEIHTGHH
       850        860        870        880        890        900
STIQYCDFSP QNHLAVVALS QYCVELWNTD SRSKVADCRG HLSWVHGVMF SPDGSSFLTS
       910        920        930        940        950        960
SDDQTIRLWE TKKVCKNSAV MLKQEVDVVF QENEVMVLAV DHIRRLQLIN GRTGQIDYLT
       970        980        990       1000       1010       1020
EAQVSCCCLS PHLQYIAFGD ENGAIEILEL VNNRIFQSRF QHKKTVWHIQ FTADEKTLIS
      1030       1040       1050       1060       1070       1080
SSDDAEIQVW NWQLDKCIFL RGHQETVKDF RLLKNSRLLS WSFDGTVKVW NIITGNKEKD
      1090       1100       1110       1120       1130       1140
FVCHQGTVLS CDISHDATKF SSTSADKTAK IWSFDLLLPL HELRGHNGCV RCSAFSVDST
      1150       1160       1170       1180       1190       1200
LLATGDDNGE IRIWNVSNGE LLHLCAPLSE EGAATHGGWV TDLCFSPDGK MLISAGGYIK
      1210       1220       1230       1240
WWNVVTGESS QTFYTNGTNL KKIHVSPDFK TYVTVDNLGI LYILQTLE         10         20         30         40         50         60
MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ QRAAMLIKMI
        70         80         90        100        110        120
LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK DSVSGITSYV RTVLCEGGVP
       130        140        150        160        170        180
QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG
       190        200        210        220        230        240
GVHWVSVGKQ DKSGLLMKLQ NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL
       250        260        270        280        290        300
ILDDVWDSWV LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN
       310        320        330        340        350        360
MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF KRIRKSSSYD
       370        380        390        400        410        420
YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL CILWDMETEE VEDILQEFVN
       430        440        450        460        470        480
KSLLFCDRNG KSFRYYLHDL QVDFLTEKNC SQLQDLHKKI ITQFQRYHQP HTLSPDQEDC
       490        500        510        520        530        540
MYWYNFLAYH MASAKMHKEL CALMFSLDWI KAKTELVGPA HLIHEFVEYR HILDEKDCAV
       550        560        570        580        590        600
SENFQEFLSL NGHLLGRQPF PNIVQLGLCE PETSEVYQQA KLQAKQEVDN GMLYLEWINK
       610        620        630        640        650        660
KNITNLSRLV VRPHTDAVYH ACFSEDGQRI ASCGADKTLQ VFKAETGEKL LEIKAHEDEV
       670        680        690        700        710        720
LCCAFSTDDR FIATCSVDKK VKIWNSMTGE LVHTYDEHSE QVNCCHFTNS SHHLLLATGS
       730        740        750        760        770        780
SDCFLKLWDL NQKECRNTMF GHTNSVNHCR FSPDDKLLAS CSADGTLKLW DATSANERKS
       790        800        810        820        830        840
INVKQFFLNL EDPQEDMEVI VKCCSWSADG ARIMVAAKNK IFLFDIHTSG LLGEIHTGHH
       850        860        870        880        890        900
STIQYCDFSP QNHLAVVALS QYCVELWNTD SRSKVADCRG HLSWVHGVMF SPDGSSFLTS
       910        920        930        940        950        960
SDDQTIRLWE TKKVCKNSAV MLKQEVDVVF QENEVMVLAV DHIRRLQLIN GRTGQIDYLT
       970        980        990       1000       1010       1020
EAQVSCCCLS PHLQYIAFGD ENGAIEILEL VNNRIFQSRF QHKKTVWHIQ FTADEKTLIS
      1030       1040       1050       1060       1070       1080
SSDDAEIQVW NWQLDKCIFL RGHQETVKDF RLLKNSRLLS WSFDGTVKVW NIITGNKEKD
      1090       1100       1110       1120       1130       1140
FVCHQGTVLS CDISHDATKF SSTSADKTAK IWSFDLLLPL HELRGHNGCV RCSAFSVDST
      1150       1160       1170       1180       1190       1200
LLATGDDNGE IRIWNVSNGE LLHLCAPLSE EGAATHGGWV TDLCFSPDGK MLISAGGYIK
      1210       1220       1230       1240
WWNVVTGESS QTFYTNGTNL KKIHVSPDFK TYVTVDNLGI LYILQTLE         10         20         30         40         50         60
MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ QRAAMLIKMI
        70         80         90        100        110        120
LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK DSVSGITSYV RTVLCEGGVP
       130        140        150        160        170        180
QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG
       190        200        210        220        230        240
GVHWVSVGKQ DKSGLLMKLQ NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL
       250        260        270        280        290        300
ILDDVWDSWV LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN
       310        320        330        340        350        360
MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF KRIRKSSSYD
       370        380        390        400        410        420
YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL CILWDMETEE VEDILQEFVN
       430        440        450        460        470        480
KSLLFCDRNG KSFRYYLHDL QVDFLTEKNC SQLQDLHKKI ITQFQRYHQP HTLSPDQEDC
       490        500        510        520        530        540
MYWYNFLAYH MASAKMHKEL CALMFSLDWI KAKTELVGPA HLIHEFVEYR HILDEKDCAV
       550        560        570        580        590        600
SENFQEFLSL NGHLLGRQPF PNIVQLGLCE PETSEVYQQA KLQAKQEVDN GMLYLEWINK
       610        620        630        640        650        660
KNITNLSRLV VRPHTDAVYH ACFSEDGQRI ASCGADKTLQ VFKAETGEKL LEIKAHEDEV
       670        680        690        700        710        720
LCCAFSTDDR FIATCSVDKK VKIWNSMTGE LVHTYDEHSE QVNCCHFTNS SHHLLLATGS
       730        740        750        760        770        780
SDCFLKLWDL NQKECRNTMF GHTNSVNHCR FSPDDKLLAS CSADGTLKLW DATSANERKS
       790        800        810        820        830        840
INVKQFFLNL EDPQEDMEVI VKCCSWSADG ARIMVAAKNK IFLFDIHTSG LLGEIHTGHH
       850        860        870        880        890        900
STIQYCDFSP QNHLAVVALS QYCVELWNTD SRSKVADCRG HLSWVHGVMF SPDGSSFLTS
       910        920        930        940        950        960
SDDQTIRLWE TKKVCKNSAV MLKQEVDVVF QENEVMVLAV DHIRRLQLIN GRTGQIDYLT
       970        980        990       1000       1010       1020
EAQVSCCCLS PHLQYIAFGD ENGAIEILEL VNNRIFQSRF QHKKTVWHIQ FTADEKTLIS
      1030       1040       1050       1060       1070       1080
SSDDAEIQVW NWQLDKCIFL RGHQETVKDF RLLKNSRLLS WSFDGTVKVW NIITGNKEKD
      1090       1100       1110       1120       1130       1140
FVCHQGTVLS CDISHDATKF SSTSADKTAK IWSFDLLLPL HELRGHNGCV RCSAFSVDST
      1150       1160       1170       1180       1190       1200
LLATGDDNGE IRIWNVSNGE LLHLCAPLSE EGAATHGGWV TDLCFSPDGK MLISAGGYIK
      1210       1220       1230       1240
WWNVVTGESS QTFYTNGTNL KKIHVSPDFK TYVTVDNLGI LYILQTLE         10         20         30         40         50         60
MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ QRAAMLIKMI
        70         80         90        100        110        120
LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK DSVSGITSYV RTVLCEGGVP
       130        140        150        160        170        180
QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG
       190        200        210        220        230        240
GVHWVSVGKQ DKSGLLMKLQ NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL
       250        260        270        280        290        300
ILDDVWDSWV LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN
       310        320        330        340        350        360
MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF KRIRKSSSYD
       370        380        390        400        410        420
YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL CILWDMETEE VEDILQEFVN
       430        440        450        460        470        480
KSLLFCDRNG KSFRYYLHDL QVDFLTEKNC SQLQDLHKKI ITQFQRYHQP HTLSPDQEDC
       490        500        510        520        530        540
MYWYNFLAYH MASAKMHKEL CALMFSLDWI KAKTELVGPA HLIHEFVEYR HILDEKDCAV
       550        560        570        580        590        600
SENFQEFLSL NGHLLGRQPF PNIVQLGLCE PETSEVYQQA KLQAKQEVDN GMLYLEWINK
       610        620        630        640        650        660
KNITNLSRLV VRPHTDAVYH ACFSEDGQRI ASCGADKTLQ VFKAETGEKL LEIKAHEDEV
       670        680        690        700        710        720
LCCAFSTDDR FIATCSVDKK VKIWNSMTGE LVHTYDEHSE QVNCCHFTNS SHHLLLATGS
       730        740        750        760        770        780
SDCFLKLWDL NQKECRNTMF GHTNSVNHCR FSPDDKLLAS CSADGTLKLW DATSANERKS
       790        800        810        820        830        840
INVKQFFLNL EDPQEDMEVI VKCCSWSADG ARIMVAAKNK IFLFDIHTSG LLGEIHTGHH
       850        860        870        880        890        900
STIQYCDFSP QNHLAVVALS QYCVELWNTD SRSKVADCRG HLSWVHGVMF SPDGSSFLTS
       910        920        930        940        950        960
SDDQTIRLWE TKKVCKNSAV MLKQEVDVVF QENEVMVLAV DHIRRLQLIN GRTGQIDYLT
       970        980        990       1000       1010       1020
EAQVSCCCLS PHLQYIAFGD ENGAIEILEL VNNRIFQSRF QHKKTVWHIQ FTADEKTLIS
      1030       1040       1050       1060       1070       1080
SSDDAEIQVW NWQLDKCIFL RGHQETVKDF RLLKNSRLLS WSFDGTVKVW NIITGNKEKD
      1090       1100       1110       1120       1130       1140
FVCHQGTVLS CDISHDATKF SSTSADKTAK IWSFDLLLPL HELRGHNGCV RCSAFSVDST
      1150       1160       1170       1180       1190       1200
LLATGDDNGE IRIWNVSNGE LLHLCAPLSE EGAATHGGWV TDLCFSPDGK MLISAGGYIK
      1210       1220       1230       1240
WWNVVTGESS QTFYTNGTNL KKIHVSPDFK TYVTVDNLGI LYILQTLE         10         20         30         40         50         60
MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ QRAAMLIKMI
        70         80         90        100        110        120
LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK DSVSGITSYV RTVLCEGGVP
       130        140        150        160        170        180
QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG
       190        200        210        220        230        240
GVHWVSVGKQ DKSGLLMKLQ NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL
       250        260        270        280        290        300
ILDDVWDSWV LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN
       310        320        330        340        350        360
MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF KRIRKSSSYD
       370        380        390        400        410        420
YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL CILWDMETEE VEDILQEFVN
       430        440        450        460        470        480
KSLLFCDRNG KSFRYYLHDL QVDFLTEKNC SQLQDLHKKI ITQFQRYHQP HTLSPDQEDC
       490        500        510        520        530        540
MYWYNFLAYH MASAKMHKEL CALMFSLDWI KAKTELVGPA HLIHEFVEYR HILDEKDCAV
       550        560        570        580        590        600
SENFQEFLSL NGHLLGRQPF PNIVQLGLCE PETSEVYQQA KLQAKQEVDN GMLYLEWINK
       610        620        630        640        650        660
KNITNLSRLV VRPHTDAVYH ACFSEDGQRI ASCGADKTLQ VFKAETGEKL LEIKAHEDEV
       670        680        690        700        710        720
LCCAFSTDDR FIATCSVDKK VKIWNSMTGE LVHTYDEHSE QVNCCHFTNS SHHLLLATGS
       730        740        750        760        770        780
SDCFLKLWDL NQKECRNTMF GHTNSVNHCR FSPDDKLLAS CSADGTLKLW DATSANERKS
       790        800        810        820        830        840
INVKQFFLNL EDPQEDMEVI VKCCSWSADG ARIMVAAKNK IFLFDIHTSG LLGEIHTGHH
       850        860        870        880        890        900
STIQYCDFSP QNHLAVVALS QYCVELWNTD SRSKVADCRG HLSWVHGVMF SPDGSSFLTS
       910        920        930        940        950        960
SDDQTIRLWE TKKVCKNSAV MLKQEVDVVF QENEVMVLAV DHIRRLQLIN GRTGQIDYLT
       970        980        990       1000       1010       1020
EAQVSCCCLS PHLQYIAFGD ENGAIEILEL VNNRIFQSRF QHKKTVWHIQ FTADEKTLIS
      1030       1040       1050       1060       1070       1080
SSDDAEIQVW NWQLDKCIFL RGHQETVKDF RLLKNSRLLS WSFDGTVKVW NIITGNKEKD
      1090       1100       1110       1120       1130       1140
FVCHQGTVLS CDISHDATKF SSTSADKTAK IWSFDLLLPL HELRGHNGCV RCSAFSVDST
      1150       1160       1170       1180       1190       1200
LLATGDDNGE IRIWNVSNGE LLHLCAPLSE EGAATHGGWV TDLCFSPDGK MLISAGGYIK
      1210       1220       1230       1240
WWNVVTGESS QTFYTNGTNL KKIHVSPDFK TYVTVDNLGI LYILQTLE

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

3 N-termini - 2 C-termini - 2 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
O14727-1-unknown	MDAKAR...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
O14727-1-unknown	MDAKAR...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt67175
O14727-1-unknown	MDAKAR...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt67176
O14727-1-unknown	MDAKAR...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt67177
O14727-1-unknown	MDAKAR...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt67178
O14727-1-unknown	MDAKAR...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt67179
O14727-1-acetylation	MDAKAR...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
O14727-1-acetylation	MDAKAR...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt178614
O14727-1-acetylation	MDAKAR...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt178615
O14727-1-acetylation	MDAKAR...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt178616
O14727-1-acetylation	MDAKAR...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt178617
O14727-1-acetylation	MDAKAR...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt178618
O14727-272-unknown	SVMGPK...	272		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1389
O14727-272-unknown	SVMGPK...	272		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2764
O14727-272-unknown	SVMGPK...	272		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt148839
O14727-272-unknown	SVMGPK...	272		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt148840
O14727-272-unknown	SVMGPK...	272		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt149175
O14727-272-unknown	SVMGPK...	272		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt149176
O14727-272-unknown	SVMGPK...	272		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt149177

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...KSVTDS	271	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC1389
...KSVTDS	271	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2764
...KSVTDS	271	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt132216
...KSVTDS	271	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt132217
...KSVTDS	271	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt132560
...KSVTDS	271	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt132561
...KSVTDS	271	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt132562
...LQTLE	1248	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...LQTLE	1248	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt62796
...LQTLE	1248	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt62794
...LQTLE	1248	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt62795
...LQTLE	1248	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt62793

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CASP3_HUMAN	271	VTDS.\|.SVMG	inferred from experiment	unknown	MEROPS	Cohen GM	Bratton SB et al.:Caspase-3 cleaves Apaf-1 into a... (C14.003)	11550094,
CASP7_HUMAN	271	VTDS.\|.SVMG	inferred from experiment	unknown	MEROPS	Cohen GM	Bratton SB et al.:Caspase-3 cleaves Apaf-1 into a... (C14.003)	11550094,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)