P35269: General transcription factor IIF subunit 1

General Information

Protein names	- General transcription factor IIF subunit 1 - General transcription factor IIF 74 kDa subunit - Transcription initiation factor IIF subunit alpha - TFIIF-alpha - Transcription initiation factor RAP74
Gene names	GTF2F1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P35269

8 N-termini

4 C-termini

1 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MAALGPSSQN VTEYVVRVPK NTTKKYNIMA FNAADKVNFA TWNQARLERD LSNKKIYQEE
        70         80         90        100        110        120
EMPESGAGSE FNRKLREEAR RKKYGIVLKE FRPEDQPWLL RVNGKSGRKF KGIKKGGVTE
       130        140        150        160        170        180
NTSYYIFTQC PDGAFEAFPV HNWYNFTPLA RHRTLTAEEA EEEWERRNKV LNHFSIMQQR
       190        200        210        220        230        240
RLKDQDQDED EEEKEKRGRR KASELRIHDL EDDLEMSSDA SDASGEEGGR VPKAKKKAPL
       250        260        270        280        290        300
AKGGRKKKKK KGSDDEAFED SDDGDFEGQE VDYMSDGSSS SQEEPESKAK APQQEEGPKG
       310        320        330        340        350        360
VDEQSDSSEE SEEEKPPEED KEEEEEKKAP TPQEKKRRKD SSEESDSSEE SDIDSEASSA
       370        380        390        400        410        420
LFMAKKKTPP KRERKPSGGS SRGNSRPGTP SAEGGSTSST LRAAASKLEQ GKRVSEMPAA
       430        440        450        460        470        480
KRLRLDTGPQ SLSGKSTPQP PSGKTTPNSG DVQVTEDAVR RYLTRKPMTT KDLLKKFQTK
       490        500        510
KTGLSSEQTV NVLAQILKRL NPERKMINDK MHFSLKE

Isoforms

Sequence View

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Evidence Names:

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Protein Neighborhood

Domains & Features

8 N-termini - 4 C-termini - 1 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P35269-2-unknown	MAALGP...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P35269-2-Acetylation	AALGPS...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P35269-2-Acetylation	AALGPS...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P35269-2-Acetylation	AALGPS...	2	acetylation-		other		CNRS/ISV	Large_scale_NTA_Human
P35269-2-Acetylation	AALGPS...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P35269-28-unknown	IMAFNA...	28		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000469091
P35269-29-unknown	MAFNAA...	29		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000392107
P35269-187-unknown	QDEDEE...	187		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11457
P35269-273-	YMSDGS...	273			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P35269-273-	YMSDGS...	273			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P35269-273-unknown	YMSDGS...	273			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P35269-425-	LDTGPQ...	425			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...SYYIFT	127	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000471542
...LKDQDQ	186	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11457
...PEEDKE	321	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000471403
...FSLKE	517	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAB_HUMAN	186	DQDQ.\|.QDED	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)