O60343: TBC1 domain family member 4

General Information

Protein names	- TBC1 domain family member 4 - Akt substrate of 160 kDa - AS160
Gene names	TBC1D4
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	O60343

11 N-termini

3 C-termini

2 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform 2 of TBC1 domain family member 4 - Isoform 3 of TBC1 domain family member 4 - Isoform 4 of TBC1 domain family member 4 - Isoform 5 of TBC1 domain family member 4

Sequence View

        10         20         30         40         50         60
MEPPSCIQDE PFPHPLEPEP GVSAQPGPGK PSDKRFRLWY VGGSCLDHRT TLPMLPWLMA
        70         80         90        100        110        120
EIRRRSQKPE AGGCGAPAAR EVILVLSAPF LRCVPAPGAG ASGGTSPSAT QPNPAVFIFE
       130        140        150        160        170        180
HKAQHISRFI HNSHDLTYFA YLIKAQPDDP ESQMACHVFR ATDPSQVPDV ISSIRQLSKA
       190        200        210        220        230        240
AMKEDAKPSK DNEDAFYNSQ KFEVLYCGKV TVTHKKAPSS LIDDCMEKFS LHEQQRLKIQ
       250        260        270        280        290        300
GEQRGPDPGE DLADLEVVVP GSPGDCLPEE ADGTDTHLGL PAGASQPALT SSRVCFPERI
       310        320        330        340        350        360
LEDSGFDEQQ EFRSRCSSVT GVQRRVHEGS QKSQPRRRHA SAPSHVQPSD SEKNRTMLFQ
       370        380        390        400        410        420
VGRFEINLIS PDTKSVVLEK NFKDISSCSQ GIKHVDHFGF ICRESPEPGL SQYICYVFQC
       430        440        450        460        470        480
ASESLVDEVM LTLKQAFSTA AALQSAKTQI KLCEACPMHS LHKLCERIEG LYPPRAKLVI
       490        500        510        520        530        540
QRHLSSLTDN EQADIFERVQ KMKPVSDQEE NELVILHLRQ LCEAKQKTHV HIGEGPSTIS
       550        560        570        580        590        600
NSTIPENATS SGRFKLDILK NKAKRSLTSS LENIFSRGAN RMRGRLGSVD SFERSNSLAS
       610        620        630        640        650        660
EKDYSPGDSP PGTPPASPPS SAWQTFPEED SDSPQFRRRA HTFSHPPSST KRKLNLQDGR
       670        680        690        700        710        720
AQGVRSPLLR QSSSEQCSNL SSVRRMYKES NSSSSLPSLH TSFSAPSFTA PSFLKSFYQN
       730        740        750        760        770        780
SGRLSPQYEN EIRQDTASES SDGEGRKRTS STCSNESLSV GGTSVTPRRI SWRQRIFLRV
       790        800        810        820        830        840
ASPMNKSPSA MQQQDGLDRN ELLPLSPLSP TMEEEPLVVF LSGEDDPEKI EERKKSKELR
       850        860        870        880        890        900
SLWRKAIHQQ ILLLRMEKEN QKLEASRDEL QSRKVKLDYE EVGACQKEVL ITWDKKLLNC
       910        920        930        940        950        960
RAKIRCDMED IHTLLKEGVP KSRRGEIWQF LALQYRLRHR LPNKQQPPDI SYKELLKQLT
       970        980        990       1000       1010       1020
AQQHAILVDL GRTFPTHPYF SVQLGPGQLS LFNLLKAYSL LDKEVGYCQG ISFVAGVLLL
      1030       1040       1050       1060       1070       1080
HMSEEQAFEM LKFLMYDLGF RKQYRPDMMS LQIQMYQLSR LLHDYHRDLY NHLEENEISP
      1090       1100       1110       1120       1130       1140
SLYAAPWFLT LFASQFSLGF VARVFDIIFL QGTEVIFKVA LSLLSSQETL IMECESFENI
      1150       1160       1170       1180       1190       1200
VEFLKNTLPD MNTSEMEKII TQVFEMDISK QLHAYEVEYH VLQDELQESS YSCEDSETLE
      1210       1220       1230       1240       1250       1260
KLERANSQLK RQNMDLLEKL QVAHTKIQAL ESNLENLLTR ETKMKSLIRT LEQEKMAYQK
      1270       1280       1290
TVEQLRKLLP ADALVNCDLL LRDLNCNPNN KAKIGNKP         10         20         30         40         50         60
MEPPSCIQDE PFPHPLEPEP GVSAQPGPGK PSDKRFRLWY VGGSCLDHRT TLPMLPWLMA
        70         80         90        100        110        120
EIRRRSQKPE AGGCGAPAAR EVILVLSAPF LRCVPAPGAG ASGGTSPSAT QPNPAVFIFE
       130        140        150        160        170        180
HKAQHISRFI HNSHDLTYFA YLIKAQPDDP ESQMACHVFR ATDPSQVPDV ISSIRQLSKA
       190        200        210        220        230        240
AMKEDAKPSK DNEDAFYNSQ KFEVLYCGKV TVTHKKAPSS LIDDCMEKFS LHEQQRLKIQ
       250        260        270        280        290        300
GEQRGPDPGE DLADLEVVVP GSPGDCLPEE ADGTDTHLGL PAGASQPALT SSRVCFPERI
       310        320        330        340        350        360
LEDSGFDEQQ EFRSRCSSVT GVQRRVHEGS QKSQPRRRHA SAPSHVQPSD SEKNRTMLFQ
       370        380        390        400        410        420
VGRFEINLIS PDTKSVVLEK NFKDISSCSQ GIKHVDHFGF ICRESPEPGL SQYICYVFQC
       430        440        450        460        470        480
ASESLVDEVM LTLKQAFSTA AALQSAKTQI KLCEACPMHS LHKLCERIEG LYPPRAKLVI
       490        500        510        520        530        540
QRHLSSLTDN EQADIFERVQ KMKPVSDQEE NELVILHLRQ LCEAKQKTHV HIGEGPSTIS
       550        560        570        580        590        600
NSTIPENATS SGRFKLDILK NKAKRSLTSS LENIFSRGAN RMRGRLGSVD SFERSNSLAS
       610        620        630        640        650        660
EKDYSPGDSP PGTPPASPPS SAWQTFPEED SDSPQFRRRA HTFSHPPSST KRKLNLQDGR
       670        680        690        700        710        720
AQGVRSPLLR QSSSEQCSNL SSVRRMYKES NSSSSLPSLH TSFSAPSFTA PSFLKSFYQN
       730        740        750        760        770        780
SGRLSPQYEN EIRQDTASES SDGEGRKRTS STCSNESLSV GGTSVTPRRI SWRQRIFLRV
       790        800        810        820        830        840
ASPMNKSPSA MQQQDGLDRN ELLPLSPLSP TMEEEPLVVF LSGEDDPEKI EERKKSKELR
       850        860        870        880        890        900
SLWRKAIHQQ ILLLRMEKEN QKLEASRDEL QSRKVKLDYE EVGACQKEVL ITWDKKLLNC
       910        920        930        940        950        960
RAKIRCDMED IHTLLKEGVP KSRRGEIWQF LALQYRLRHR LPNKQQPPDI SYKELLKQLT
       970        980        990       1000       1010       1020
AQQHAILVDL GRTFPTHPYF SVQLGPGQLS LFNLLKAYSL LDKEVGYCQG ISFVAGVLLL
      1030       1040       1050       1060       1070       1080
HMSEEQAFEM LKFLMYDLGF RKQYRPDMMS LQIQMYQLSR LLHDYHRDLY NHLEENEISP
      1090       1100       1110       1120       1130       1140
SLYAAPWFLT LFASQFSLGF VARVFDIIFL QGTEVIFKVA LSLLSSQETL IMECESFENI
      1150       1160       1170       1180       1190       1200
VEFLKNTLPD MNTSEMEKII TQVFEMDISK QLHAYEVEYH VLQDELQESS YSCEDSETLE
      1210       1220       1230       1240       1250       1260
KLERANSQLK RQNMDLLEKL QVAHTKIQAL ESNLENLLTR ETKMKSLIRT LEQEKMAYQK
      1270       1280       1290
TVEQLRKLLP ADALVNCDLL LRDLNCNPNN KAKIGNKP         10         20         30         40         50         60
MEPPSCIQDE PFPHPLEPEP GVSAQPGPGK PSDKRFRLWY VGGSCLDHRT TLPMLPWLMA
        70         80         90        100        110        120
EIRRRSQKPE AGGCGAPAAR EVILVLSAPF LRCVPAPGAG ASGGTSPSAT QPNPAVFIFE
       130        140        150        160        170        180
HKAQHISRFI HNSHDLTYFA YLIKAQPDDP ESQMACHVFR ATDPSQVPDV ISSIRQLSKA
       190        200        210        220        230        240
AMKEDAKPSK DNEDAFYNSQ KFEVLYCGKV TVTHKKAPSS LIDDCMEKFS LHEQQRLKIQ
       250        260        270        280        290        300
GEQRGPDPGE DLADLEVVVP GSPGDCLPEE ADGTDTHLGL PAGASQPALT SSRVCFPERI
       310        320        330        340        350        360
LEDSGFDEQQ EFRSRCSSVT GVQRRVHEGS QKSQPRRRHA SAPSHVQPSD SEKNRTMLFQ
       370        380        390        400        410        420
VGRFEINLIS PDTKSVVLEK NFKDISSCSQ GIKHVDHFGF ICRESPEPGL SQYICYVFQC
       430        440        450        460        470        480
ASESLVDEVM LTLKQAFSTA AALQSAKTQI KLCEACPMHS LHKLCERIEG LYPPRAKLVI
       490        500        510        520        530        540
QRHLSSLTDN EQADIFERVQ KMKPVSDQEE NELVILHLRQ LCEAKQKTHV HIGEGPSTIS
       550        560        570        580        590        600
NSTIPENATS SGRFKLDILK NKAKRSLTSS LENIFSRGAN RMRGRLGSVD SFERSNSLAS
       610        620        630        640        650        660
EKDYSPGDSP PGTPPASPPS SAWQTFPEED SDSPQFRRRA HTFSHPPSST KRKLNLQDGR
       670        680        690        700        710        720
AQGVRSPLLR QSSSEQCSNL SSVRRMYKES NSSSSLPSLH TSFSAPSFTA PSFLKSFYQN
       730        740        750        760        770        780
SGRLSPQYEN EIRQDTASES SDGEGRKRTS STCSNESLSV GGTSVTPRRI SWRQRIFLRV
       790        800        810        820        830        840
ASPMNKSPSA MQQQDGLDRN ELLPLSPLSP TMEEEPLVVF LSGEDDPEKI EERKKSKELR
       850        860        870        880        890        900
SLWRKAIHQQ ILLLRMEKEN QKLEASRDEL QSRKVKLDYE EVGACQKEVL ITWDKKLLNC
       910        920        930        940        950        960
RAKIRCDMED IHTLLKEGVP KSRRGEIWQF LALQYRLRHR LPNKQQPPDI SYKELLKQLT
       970        980        990       1000       1010       1020
AQQHAILVDL GRTFPTHPYF SVQLGPGQLS LFNLLKAYSL LDKEVGYCQG ISFVAGVLLL
      1030       1040       1050       1060       1070       1080
HMSEEQAFEM LKFLMYDLGF RKQYRPDMMS LQIQMYQLSR LLHDYHRDLY NHLEENEISP
      1090       1100       1110       1120       1130       1140
SLYAAPWFLT LFASQFSLGF VARVFDIIFL QGTEVIFKVA LSLLSSQETL IMECESFENI
      1150       1160       1170       1180       1190       1200
VEFLKNTLPD MNTSEMEKII TQVFEMDISK QLHAYEVEYH VLQDELQESS YSCEDSETLE
      1210       1220       1230       1240       1250       1260
KLERANSQLK RQNMDLLEKL QVAHTKIQAL ESNLENLLTR ETKMKSLIRT LEQEKMAYQK
      1270       1280       1290
TVEQLRKLLP ADALVNCDLL LRDLNCNPNN KAKIGNKP         10         20         30         40         50         60
MEPPSCIQDE PFPHPLEPEP GVSAQPGPGK PSDKRFRLWY VGGSCLDHRT TLPMLPWLMA
        70         80         90        100        110        120
EIRRRSQKPE AGGCGAPAAR EVILVLSAPF LRCVPAPGAG ASGGTSPSAT QPNPAVFIFE
       130        140        150        160        170        180
HKAQHISRFI HNSHDLTYFA YLIKAQPDDP ESQMACHVFR ATDPSQVPDV ISSIRQLSKA
       190        200        210        220        230        240
AMKEDAKPSK DNEDAFYNSQ KFEVLYCGKV TVTHKKAPSS LIDDCMEKFS LHEQQRLKIQ
       250        260        270        280        290        300
GEQRGPDPGE DLADLEVVVP GSPGDCLPEE ADGTDTHLGL PAGASQPALT SSRVCFPERI
       310        320        330        340        350        360
LEDSGFDEQQ EFRSRCSSVT GVQRRVHEGS QKSQPRRRHA SAPSHVQPSD SEKNRTMLFQ
       370        380        390        400        410        420
VGRFEINLIS PDTKSVVLEK NFKDISSCSQ GIKHVDHFGF ICRESPEPGL SQYICYVFQC
       430        440        450        460        470        480
ASESLVDEVM LTLKQAFSTA AALQSAKTQI KLCEACPMHS LHKLCERIEG LYPPRAKLVI
       490        500        510        520        530        540
QRHLSSLTDN EQADIFERVQ KMKPVSDQEE NELVILHLRQ LCEAKQKTHV HIGEGPSTIS
       550        560        570        580        590        600
NSTIPENATS SGRFKLDILK NKAKRSLTSS LENIFSRGAN RMRGRLGSVD SFERSNSLAS
       610        620        630        640        650        660
EKDYSPGDSP PGTPPASPPS SAWQTFPEED SDSPQFRRRA HTFSHPPSST KRKLNLQDGR
       670        680        690        700        710        720
AQGVRSPLLR QSSSEQCSNL SSVRRMYKES NSSSSLPSLH TSFSAPSFTA PSFLKSFYQN
       730        740        750        760        770        780
SGRLSPQYEN EIRQDTASES SDGEGRKRTS STCSNESLSV GGTSVTPRRI SWRQRIFLRV
       790        800        810        820        830        840
ASPMNKSPSA MQQQDGLDRN ELLPLSPLSP TMEEEPLVVF LSGEDDPEKI EERKKSKELR
       850        860        870        880        890        900
SLWRKAIHQQ ILLLRMEKEN QKLEASRDEL QSRKVKLDYE EVGACQKEVL ITWDKKLLNC
       910        920        930        940        950        960
RAKIRCDMED IHTLLKEGVP KSRRGEIWQF LALQYRLRHR LPNKQQPPDI SYKELLKQLT
       970        980        990       1000       1010       1020
AQQHAILVDL GRTFPTHPYF SVQLGPGQLS LFNLLKAYSL LDKEVGYCQG ISFVAGVLLL
      1030       1040       1050       1060       1070       1080
HMSEEQAFEM LKFLMYDLGF RKQYRPDMMS LQIQMYQLSR LLHDYHRDLY NHLEENEISP
      1090       1100       1110       1120       1130       1140
SLYAAPWFLT LFASQFSLGF VARVFDIIFL QGTEVIFKVA LSLLSSQETL IMECESFENI
      1150       1160       1170       1180       1190       1200
VEFLKNTLPD MNTSEMEKII TQVFEMDISK QLHAYEVEYH VLQDELQESS YSCEDSETLE
      1210       1220       1230       1240       1250       1260
KLERANSQLK RQNMDLLEKL QVAHTKIQAL ESNLENLLTR ETKMKSLIRT LEQEKMAYQK
      1270       1280       1290
TVEQLRKLLP ADALVNCDLL LRDLNCNPNN KAKIGNKP

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Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

11 N-termini - 3 C-termini - 2 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
O60343-1-Acetylation	MEPPSC...	1	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
O60343-1-Acetylation	MEPPSC...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
O60343-1-Acetylation	MEPPSC...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt103224
O60343-1-Acetylation	MEPPSC...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt103225
O60343-1-unknown	MEPPSC...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt91623
O60343-1-unknown	MEPPSC...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt91624
O60343-273-unknown	GTDTHL...	273			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
O60343-273-unknown	GTDTHL...	273		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt162191
O60343-273-unknown	GTDTHL...	273		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt162192
O60343-273-	GTDTHL...	273			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
O60343-273-	GTDTHL...	273			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
O60343-273-	GTDTHL...	273			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O60343-273-	GTDTHL...	273			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
O60343-276-unknown	THLGLP...	276		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt167352
O60343-276-unknown	THLGLP...	276		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt167353
O60343-276-	THLGLP...	276			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
O60343-276-	THLGLP...	276			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O60343-276-	THLGLP...	276			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
O60343-279-	GLPAGA...	279			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
O60343-279-	GLPAGA...	279			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O60343-279-unknown	GLPAGA...	279			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
O60343-279-unknown	GLPAGA...	279		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt162188
O60343-279-unknown	GLPAGA...	279		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt162189
O60343-304-unknown	SGFDEQ...	304		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10815
O60343-304-unknown	SGFDEQ...	304		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt149972
O60343-304-unknown	SGFDEQ...	304		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt149973
O60343-784-unknown	MNKSPS...	784		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt91625
O60343-784-unknown	MNKSPS...	784		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt91626
O60343-784-unknown	MNKSPS...	784		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt108814
O60343-784-unknown	MNKSPS...	784		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt108813
O60343-822-unknown	SGEDDP...	822		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10818
O60343-822-unknown	SGEDDP...	822		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt138243
O60343-822-unknown	SGEDDP...	822		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt138244
O60343-822-unknown	SGEDDP...	822		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt156304
O60343-822-unknown	SGEDDP...	822		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt156550

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...RILEDS	303	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10815
...RILEDS	303	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt133364
...RILEDS	303	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt133365
...LVVFLS	821	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10818
...LVVFLS	821	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt121274
...LVVFLS	821	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt121275
...LVVFLS	821	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt139776
...LVVFLS	821	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt140034
...IGNKP	1298	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...IGNKP	1298	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt87243
...IGNKP	1298	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt87244
...IGNKP	1298	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt87241
...IGNKP	1298	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt87242

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAB_HUMAN	303	LEDS.\|.SGFD	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	821	VFLS.\|.SGED	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)