P46459: Vesicle-fusing ATPase

General Information

Protein names	- Vesicle-fusing ATPase - 3.6.4.6 - N-ethylmaleimide-sensitive fusion protein - NEM-sensitive fusion protein - Vesicular-fusion protein NSF
Gene names	NSF
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P46459

7 N-termini

7 C-termini

2 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MAGRSMQAAR CPTDELSLTN CAVVNEKDFQ SGQHVIVRTS PNHRYTFTLK THPSVVPGSI
        70         80         90        100        110        120
AFSLPQRKWA GLSIGQEIEV SLYTFDKAKQ CIGTMTIEID FLQKKSIDSN PYDTDKMAAE
       130        140        150        160        170        180
FIQQFNNQAF SVGQQLVFSF NEKLFGLLVK DIEAMDPSIL KGEPATGKRQ KIEVGLVVGN
       190        200        210        220        230        240
SQVAFEKAEN SSLNLIGKAK TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF
       250        260        270        280        290        300
PPEIVEQMGC KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA
       310        320        330        340        350        360
NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV VNQLLSKIDG
       370        380        390        400        410        420
VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE KGRLQILHIH TARMRGHQLL
       430        440        450        460        470        480
SADVDIKELA VETKNFSGAE LEGLVRAAQS TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF
       490        500        510        520        530        540
LASLENDIKP AFGTNQEDYA SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL
       550        560        570        580        590        600
LEGPPHSGKT ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV
       610        620        630        640        650        660
VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD VLQEMEMLNA
       670        680        690        700        710        720
FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG KKVWIGIKKL LMLIEMSLQM
       730        740
DPEYRVRKFL ALLREEGASP LDFD

Isoforms

- Isoform 2 of Vesicle-fusing ATPase

Sequence View

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Evidence Codes:

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Protein Neighborhood

Domains & Features

7 N-termini - 7 C-termini - 2 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P46459-1-unknown	MAGRSM...	1	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P46459-6-unknown	MQAARC...	6	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000460152
P46459-6-unknown	MQAARC...	6	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000458184
P46459-6-unknown	MQAARC...	6	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000458221
P46459-95-unknown	MTIEID...	95	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000225282
P46459-105-	KSIDSN...	105		Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P46459-105-	KSIDSN...	105		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P46459-176-unknown	LVVGNS...	176	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3805
P46459-467-unknown	MEKAES...	467	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11529
P46459-573-unknown	MIGFSE...	573	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000459646

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...YTFTLK	49	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000460152
...GEPATG	166	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000458184
...KIEVGL	175	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3805
...KVEVDM	466	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11529
...WIGIKK	708	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000459646
...REEGAS	738	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000225282
...PLDFD	744	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...PLDFD	744		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...PLDFD	744		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CATB_HUMAN	175	EVGL.\|.LVVG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
GRAB_HUMAN	466	EVDM.\|.MEKA	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)