Q9H3P2: Negative elongation factor A

General Information

Protein names	- Negative elongation factor A - NELF-A - Wolf-Hirschhorn syndrome candidate 2 protein
Gene names	NELFA
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	Q9H3P2

18 N-termini

6 C-termini

1 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MASMRESDTG LWLHNKLGAT DELWAPPSIA SLLTAAVIDN IRLCFHGLSS AVKLKLLLGT
        70         80         90        100        110        120
LHLPRRTVDE MKGALMEIIQ LASLDSDPWV LMVADILKSF PDTGSLNLEL EEQNPNVQDI
       130        140        150        160        170        180
LGELREKVGE CEASAMLPLE CQYLNKNALT TLAGPLTPPV KHFQLKRKPK SATLRAELLQ
       190        200        210        220        230        240
KSTETAQQLK RSAGVPFHAK GRGLLRKMDT TTPLKGIPKQ APFRSPTAPS VFSPTGNRTP
       250        260        270        280        290        300
IPPSRTLLRK ERGVKLLDIS ELDMVGAGRE AKRRRKTLDA EVVEKPAKEE TVVENATPDY
       310        320        330        340        350        360
AAGLVSTQKL GSLNNEPALP STSYLPSTPS VVPASSYIPS SETPPAPSSR EASRPPEEPS
       370        380        390        400        410        420
APSPTLPAQF KQRAPMYNSG LSPATPTPAA PTSPLTPTTP PAVAPTTQTP PVAMVAPQTQ
       430        440        450        460        470        480
APAQQQPKKN LSLTREQMFA AQEMFKTANK VTRPEKALIL GFMAGSRENP CQEQGDVIQI
       490        500        510        520
KLSEHTEDLP KADGQGSTTM LVDTVFEMNY ATGQWTRFKK YKPMTNVS

Isoforms

- Isoform 2 of Negative elongation factor A

Sequence View

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

18 N-termini - 6 C-termini - 1 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
Q9H3P2-1-unknown	MASMRE...	1	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
Q9H3P2-71-unknown	MKGALM...	71	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000410154
Q9H3P2-136-unknown	MLPLEC...	136	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000445757
Q9H3P2-208-	MDTTTP...	208		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
Q9H3P2-229-unknown	PSVFSP...	229		unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
Q9H3P2-264-unknown	MVGAGR...	264	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt82600
Q9H3P2-300-	YAAGLV...	300		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
Q9H3P2-300-	YAAGLV...	300		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
Q9H3P2-300-	YAAGLV...	300		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q9H3P2-300-	YAAGLV...	300		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q9H3P2-300-unknown	YAAGLV...	300	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt166006
Q9H3P2-301-	AAGLVS...	301		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q9H3P2-301-unknown	AAGLVS...	301	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175908
Q9H3P2-321-unknown	STSYLP...	321		unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
Q9H3P2-321-unknown	STSYLP...	321	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt161866
Q9H3P2-337-	YIPSSE...	337		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
Q9H3P2-337-unknown	YIPSSE...	337	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168315
Q9H3P2-414-unknown	MVAPQT...	414	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt172480
Q9H3P2-414-	MVAPQT...	414		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q9H3P2-415-unknown	VAPQTQ...	415	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC14995
Q9H3P2-415-unknown	VAPQTQ...	415	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt144827
Q9H3P2-461-	GFMAGS...	461		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
Q9H3P2-461-unknown	GFMAGS...	461	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt178464

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...AKGRGL	203	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000396996
...RRKTLD	278	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000395761
...RRKTLD	278	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000410154
...SETPPA	345	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000405119
...SETPPA	345	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt114478
...PPVAMV	414	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC14995
...PPVAMV	414	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt128103
...MTNVS	528		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...MTNVS	528	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...MTNVS	528	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt78218
...MTNVS	528		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAM_HUMAN	414	VAMV.\|.VAPQ	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)