O14745: Na(+)/H(+) exchange regulatory cofactor NHE-RF1

General Information

Protein names	- Na(+)/H(+) exchange regulatory cofactor NHE-RF1 - NHERF-1 - Ezrin-radixin-moesin-binding phosphoprotein 50 - EBP50 - Regulatory cofactor of Na(+)/H(+) exchanger - Sodium-hydrogen exchanger regulatory factor 1 - Solute carrier family 9 isoform A3 regulatory factor 1
Gene names	SLC9A3R1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	O14745

10 N-termini

4 C-termini

1 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MSADAAAGAP LPRLCCLEKG PNGYGFHLHG EKGKLGQYIR LVEPGSPAEK AGLLAGDRLV
        70         80         90        100        110        120
EVNGENVEKE THQQVVSRIR AALNAVRLLV VDPETDEQLQ KLGVQVREEL LRAQEAPGQA
       130        140        150        160        170        180
EPPAAAEVQG AGNENEPREA DKSHPEQREL RPRLCTMKKG PSGYGFNLHS DKSKPGQFIR
       190        200        210        220        230        240
SVDPDSPAEA SGLRAQDRIV EVNGVCMEGK QHGDVVSAIR AGGDETKLLV VDRETDEFFK
       250        260        270        280        290        300
KCRVIPSQEH LNGPLPVPFT NGEIQKENSR EALAEAALES PRPALVRSAS SDTSEELNSQ
       310        320        330        340        350
DSPPKQDSTA PSSTSSSDPI LDFNISLAMA KERAHQKRSS KRAPQMDWSK KNELFSNL

Isoforms

- Isoform 2 of Na(+)/H(+) exchange regulatory cofactor NHE-RF1

Sequence View

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Protein Neighborhood

Domains & Features

10 N-termini - 4 C-termini - 1 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
O14745-2-unknown	MSADAA...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
O14745-2-Acetylation	SADAAA...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
O14745-2-Acetylation	SADAAA...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
O14745-2-Acetylation	SADAAA...	2	acetylation-		other		CNRS/ISV	Large_scale_NTA_Human
O14745-2-Acetylation	SADAAA...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
O14745-5-	AAAGAP...	5			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O14745-5-	AAAGAP...	5			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
O14745-5-	AAAGAP...	5			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
O14745-121-unknown	EPPAAA...	121		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13711
O14745-124-	AAAEVQ...	124			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
O14745-124-	AAAEVQ...	124			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
O14745-124-	AAAEVQ...	124			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
O14745-157-unknown	MKKGPS...	157		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000464982
O14745-181-unknown	SVDPDS...	181			unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
O14745-181-	SVDPDS...	181			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
O14745-181-	SVDPDS...	181			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
O14745-274-	AEAALE...	274			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O14745-276-	AALESP...	276			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O14745-276-	AALESP...	276			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...APGQAE	120	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13711
...AKERAH	334	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000464321
...LFSNL	358		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...LFSNL	358	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...LFSNL	358		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAM_HUMAN	120	GQAE.\|.EPPA	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)