O14974: Protein phosphatase 1 regulatory subunit 12A

General Information

Protein names	- Protein phosphatase 1 regulatory subunit 12A - Myosin phosphatase-targeting subunit 1 - Myosin phosphatase target subunit 1 - Protein phosphatase myosin-binding subunit
Gene names	PPP1R12A
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	O14974

9 N-termini

3 C-termini

1 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform 2 of Protein phosphatase 1 regulatory subunit 12A - Isoform 3 of Protein phosphatase 1 regulatory subunit 12A - Isoform 4 of Protein phosphatase 1 regulatory subunit 12A - Isoform 5 of Protein phosphatase 1 regulatory subunit 12A

Sequence View

        10         20         30         40         50         60
MKMADAKQKR NEQLKRWIGS ETDLEPPVVK RQKTKVKFDD GAVFLAACSS GDTDEVLKLL
        70         80         90        100        110        120
HRGADINYAN VDGLTALHQA CIDDNVDMVK FLVENGANIN QPDNEGWIPL HAAASCGYLD
       130        140        150        160        170        180
IAEFLIGQGA HVGAVNSEGD TPLDIAEEEA MEELLQNEVN RQGVDIEAAR KEEERIMLRD
       190        200        210        220        230        240
ARQWLNSGHI NDVRHAKSGG TALHVAAAKG YTEVLKLLIQ AGYDVNIKDY DGWTPLHAAA
       250        260        270        280        290        300
HWGKEEACRI LVDNLCDMEM VNKVGQTAFD VADEDILGYL EELQKKQNLL HSEKRDKKSP
       310        320        330        340        350        360
LIESTANMDN NQSQKTFKNK ETLIIEPEKN ASRIESLEQE KVDEEEEGKK DESSCSSEED
       370        380        390        400        410        420
EEDDSESEAE TDKTKPLASV TNANTSSTQA APVAVTTPTV SSGQATPTSP IKKFPTTATK
       430        440        450        460        470        480
ISPKEEERKD ESPATWRLGL RKTGSYGALA EITASKEGQK EKDTAGVTRS ASSPRLSSSL
       490        500        510        520        530        540
DNKEKEKDSK GTRLAYVAPT IPRRLASTSD IEEKENRDSS SLRTSSSYTR RKWEDDLKKN
       550        560        570        580        590        600
SSVNEGSTYH KSCSFGRRQD DLISSSVPST TSTPTVTSAA GLQKSLLSST STTTKITTGS
       610        620        630        640        650        660
SSAGTQSSTS NRLWAEDSTE KEKDSVPTAV TIPVAPTVVN AAASTTTLTT TTAGTVSSTT
       670        680        690        700        710        720
EVRERRRSYL TPVRDEESES QRKARSRQAR QSRRSTQGVT LTDLQEAEKT IGRSRSTRTR
       730        740        750        760        770        780
EQENEEKEKE EKEKQDKEKQ EEKKESETSR EDEYKQKYSR TYDETYQRYR PVSTSSSTTP
       790        800        810        820        830        840
SSSLSTMSSS LYASSQLNRP NSLVGITSAY SRGITKENER EGEKREEEKE GEDKSQPKSI
       850        860        870        880        890        900
RERRRPREKR RSTGVSFWTQ DSDENEQEQQ SDTEEGSNKK ETQTDSISRY ETSSTSAGDR
       910        920        930        940        950        960
YDSLLGRSGS YSYLEERKPY SSRLEKDDST DFKKLYEQIL AENEKLKAQL HDTNMELTDL
       970        980        990       1000       1010       1020
KLQLEKATQR QERFADRSLL EMEKRERRAL ERRISEMEEE LKMLPDLKAD NQRLKDENGA
      1030
LIRVISKLSK         10         20         30         40         50         60
MKMADAKQKR NEQLKRWIGS ETDLEPPVVK RQKTKVKFDD GAVFLAACSS GDTDEVLKLL
        70         80         90        100        110        120
HRGADINYAN VDGLTALHQA CIDDNVDMVK FLVENGANIN QPDNEGWIPL HAAASCGYLD
       130        140        150        160        170        180
IAEFLIGQGA HVGAVNSEGD TPLDIAEEEA MEELLQNEVN RQGVDIEAAR KEEERIMLRD
       190        200        210        220        230        240
ARQWLNSGHI NDVRHAKSGG TALHVAAAKG YTEVLKLLIQ AGYDVNIKDY DGWTPLHAAA
       250        260        270        280        290        300
HWGKEEACRI LVDNLCDMEM VNKVGQTAFD VADEDILGYL EELQKKQNLL HSEKRDKKSP
       310        320        330        340        350        360
LIESTANMDN NQSQKTFKNK ETLIIEPEKN ASRIESLEQE KVDEEEEGKK DESSCSSEED
       370        380        390        400        410        420
EEDDSESEAE TDKTKPLASV TNANTSSTQA APVAVTTPTV SSGQATPTSP IKKFPTTATK
       430        440        450        460        470        480
ISPKEEERKD ESPATWRLGL RKTGSYGALA EITASKEGQK EKDTAGVTRS ASSPRLSSSL
       490        500        510        520        530        540
DNKEKEKDSK GTRLAYVAPT IPRRLASTSD IEEKENRDSS SLRTSSSYTR RKWEDDLKKN
       550        560        570        580        590        600
SSVNEGSTYH KSCSFGRRQD DLISSSVPST TSTPTVTSAA GLQKSLLSST STTTKITTGS
       610        620        630        640        650        660
SSAGTQSSTS NRLWAEDSTE KEKDSVPTAV TIPVAPTVVN AAASTTTLTT TTAGTVSSTT
       670        680        690        700        710        720
EVRERRRSYL TPVRDEESES QRKARSRQAR QSRRSTQGVT LTDLQEAEKT IGRSRSTRTR
       730        740        750        760        770        780
EQENEEKEKE EKEKQDKEKQ EEKKESETSR EDEYKQKYSR TYDETYQRYR PVSTSSSTTP
       790        800        810        820        830        840
SSSLSTMSSS LYASSQLNRP NSLVGITSAY SRGITKENER EGEKREEEKE GEDKSQPKSI
       850        860        870        880        890        900
RERRRPREKR RSTGVSFWTQ DSDENEQEQQ SDTEEGSNKK ETQTDSISRY ETSSTSAGDR
       910        920        930        940        950        960
YDSLLGRSGS YSYLEERKPY SSRLEKDDST DFKKLYEQIL AENEKLKAQL HDTNMELTDL
       970        980        990       1000       1010       1020
KLQLEKATQR QERFADRSLL EMEKRERRAL ERRISEMEEE LKMLPDLKAD NQRLKDENGA
      1030
LIRVISKLSK         10         20         30         40         50         60
MKMADAKQKR NEQLKRWIGS ETDLEPPVVK RQKTKVKFDD GAVFLAACSS GDTDEVLKLL
        70         80         90        100        110        120
HRGADINYAN VDGLTALHQA CIDDNVDMVK FLVENGANIN QPDNEGWIPL HAAASCGYLD
       130        140        150        160        170        180
IAEFLIGQGA HVGAVNSEGD TPLDIAEEEA MEELLQNEVN RQGVDIEAAR KEEERIMLRD
       190        200        210        220        230        240
ARQWLNSGHI NDVRHAKSGG TALHVAAAKG YTEVLKLLIQ AGYDVNIKDY DGWTPLHAAA
       250        260        270        280        290        300
HWGKEEACRI LVDNLCDMEM VNKVGQTAFD VADEDILGYL EELQKKQNLL HSEKRDKKSP
       310        320        330        340        350        360
LIESTANMDN NQSQKTFKNK ETLIIEPEKN ASRIESLEQE KVDEEEEGKK DESSCSSEED
       370        380        390        400        410        420
EEDDSESEAE TDKTKPLASV TNANTSSTQA APVAVTTPTV SSGQATPTSP IKKFPTTATK
       430        440        450        460        470        480
ISPKEEERKD ESPATWRLGL RKTGSYGALA EITASKEGQK EKDTAGVTRS ASSPRLSSSL
       490        500        510        520        530        540
DNKEKEKDSK GTRLAYVAPT IPRRLASTSD IEEKENRDSS SLRTSSSYTR RKWEDDLKKN
       550        560        570        580        590        600
SSVNEGSTYH KSCSFGRRQD DLISSSVPST TSTPTVTSAA GLQKSLLSST STTTKITTGS
       610        620        630        640        650        660
SSAGTQSSTS NRLWAEDSTE KEKDSVPTAV TIPVAPTVVN AAASTTTLTT TTAGTVSSTT
       670        680        690        700        710        720
EVRERRRSYL TPVRDEESES QRKARSRQAR QSRRSTQGVT LTDLQEAEKT IGRSRSTRTR
       730        740        750        760        770        780
EQENEEKEKE EKEKQDKEKQ EEKKESETSR EDEYKQKYSR TYDETYQRYR PVSTSSSTTP
       790        800        810        820        830        840
SSSLSTMSSS LYASSQLNRP NSLVGITSAY SRGITKENER EGEKREEEKE GEDKSQPKSI
       850        860        870        880        890        900
RERRRPREKR RSTGVSFWTQ DSDENEQEQQ SDTEEGSNKK ETQTDSISRY ETSSTSAGDR
       910        920        930        940        950        960
YDSLLGRSGS YSYLEERKPY SSRLEKDDST DFKKLYEQIL AENEKLKAQL HDTNMELTDL
       970        980        990       1000       1010       1020
KLQLEKATQR QERFADRSLL EMEKRERRAL ERRISEMEEE LKMLPDLKAD NQRLKDENGA
      1030
LIRVISKLSK         10         20         30         40         50         60
MKMADAKQKR NEQLKRWIGS ETDLEPPVVK RQKTKVKFDD GAVFLAACSS GDTDEVLKLL
        70         80         90        100        110        120
HRGADINYAN VDGLTALHQA CIDDNVDMVK FLVENGANIN QPDNEGWIPL HAAASCGYLD
       130        140        150        160        170        180
IAEFLIGQGA HVGAVNSEGD TPLDIAEEEA MEELLQNEVN RQGVDIEAAR KEEERIMLRD
       190        200        210        220        230        240
ARQWLNSGHI NDVRHAKSGG TALHVAAAKG YTEVLKLLIQ AGYDVNIKDY DGWTPLHAAA
       250        260        270        280        290        300
HWGKEEACRI LVDNLCDMEM VNKVGQTAFD VADEDILGYL EELQKKQNLL HSEKRDKKSP
       310        320        330        340        350        360
LIESTANMDN NQSQKTFKNK ETLIIEPEKN ASRIESLEQE KVDEEEEGKK DESSCSSEED
       370        380        390        400        410        420
EEDDSESEAE TDKTKPLASV TNANTSSTQA APVAVTTPTV SSGQATPTSP IKKFPTTATK
       430        440        450        460        470        480
ISPKEEERKD ESPATWRLGL RKTGSYGALA EITASKEGQK EKDTAGVTRS ASSPRLSSSL
       490        500        510        520        530        540
DNKEKEKDSK GTRLAYVAPT IPRRLASTSD IEEKENRDSS SLRTSSSYTR RKWEDDLKKN
       550        560        570        580        590        600
SSVNEGSTYH KSCSFGRRQD DLISSSVPST TSTPTVTSAA GLQKSLLSST STTTKITTGS
       610        620        630        640        650        660
SSAGTQSSTS NRLWAEDSTE KEKDSVPTAV TIPVAPTVVN AAASTTTLTT TTAGTVSSTT
       670        680        690        700        710        720
EVRERRRSYL TPVRDEESES QRKARSRQAR QSRRSTQGVT LTDLQEAEKT IGRSRSTRTR
       730        740        750        760        770        780
EQENEEKEKE EKEKQDKEKQ EEKKESETSR EDEYKQKYSR TYDETYQRYR PVSTSSSTTP
       790        800        810        820        830        840
SSSLSTMSSS LYASSQLNRP NSLVGITSAY SRGITKENER EGEKREEEKE GEDKSQPKSI
       850        860        870        880        890        900
RERRRPREKR RSTGVSFWTQ DSDENEQEQQ SDTEEGSNKK ETQTDSISRY ETSSTSAGDR
       910        920        930        940        950        960
YDSLLGRSGS YSYLEERKPY SSRLEKDDST DFKKLYEQIL AENEKLKAQL HDTNMELTDL
       970        980        990       1000       1010       1020
KLQLEKATQR QERFADRSLL EMEKRERRAL ERRISEMEEE LKMLPDLKAD NQRLKDENGA
      1030
LIRVISKLSK

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

9 N-termini - 3 C-termini - 1 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
O14974-1-unknown	MKMADA...	1	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
O14974-1-unknown	MKMADA...	1	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt82102
O14974-1-unknown	MKMADA...	1	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt82103
O14974-1-unknown	MKMADA...	1	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt82104
O14974-1-unknown	MKMADA...	1		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
O14974-1-unknown	MKMADA...	1		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
O14974-88-unknown	MVKFLV...	88	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt82105
O14974-88-unknown	MVKFLV...	88	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt106217
O14974-88-unknown	MVKFLV...	88	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt106218
O14974-88-unknown	MVKFLV...	88	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt106219
O14974-572-	STPTVT...	572		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
O14974-572-	STPTVT...	572		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
O14974-572-	STPTVT...	572		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
O14974-572-unknown	STPTVT...	572	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt167728
O14974-572-unknown	STPTVT...	572	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt167729
O14974-572-unknown	STPTVT...	572	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt167730
O14974-585-	SLLSST...	585		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
O14974-585-unknown	SLLSST...	585		unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
O14974-585-unknown	SLLSST...	585	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt159940
O14974-585-unknown	SLLSST...	585	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt159941
O14974-585-unknown	SLLSST...	585	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt159942
O14974-588-unknown	SSTSTT...	588	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13714
O14974-588-unknown	SSTSTT...	588	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt153422
O14974-588-unknown	SSTSTT...	588	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt154435
O14974-886-	SISRYE...	886		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
O14974-886-	SISRYE...	886		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O14974-886-	SISRYE...	886		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
O14974-886-	SISRYE...	886		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
O14974-886-unknown	SISRYE...	886	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt167723
O14974-886-unknown	SISRYE...	886	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt167724
O14974-886-unknown	SISRYE...	886	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt167725
O14974-886-unknown	SISRYE...	886	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt167726

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...QKSLLS	587	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13714
...QKSLLS	587	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt136845
...QKSLLS	587	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt137855
...QKSLLS	587	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt137856
...SKLSK	1030	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...SKLSK	1030	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt77723
...SKLSK	1030	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt77722
...SKLSK	1030	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt77721
...SKLSK	1030	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt77720
...SKLSK	1030		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...SKLSK	1030		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...SKLSK	1030		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...SKLSK	1030		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAM_HUMAN	587	SLLS.\|.SSTS	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)