O95104: SR-related and CTD-associated factor 4 {ECO:0000303|PubMed:31104839}

General Information

Protein names	- SR-related and CTD-associated factor 4 {ECO:0000303\|PubMed:31104839} - CTD-binding SR-like protein RA4 {ECO:0000305} - Splicing factor, arginine/serine-rich 15 {ECO:0000305}
Gene names	SCAF4
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	O95104

9 N-termini

4 C-termini

3 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform 2 of Splicing factor, arginine/serine-rich 15 - Isoform 3 of Splicing factor, arginine/serine-rich 15 - Isoform 2 of SR-related and CTD-associated factor 4 - Isoform 3 of SR-related and CTD-associated factor 4

Sequence View

        10         20         30         40         50         60
MDAVNAFNQE LFSLMDMKPP ISRAKMILIT KAAIKAIKLY KHVVQIVEKF IKKCKPEYKV
        70         80         90        100        110        120
PGLYVIDSIV RQSRHQFGTD KDVFGPRFSK NITATFQYLY LCPSEDKSKI VRVLNLWQKN
       130        140        150        160        170        180
GVFKIEIIQP LLDMAAGTSN AAPVAENVTN NEGSPPPPVK VSSEPPTQAT PNSVPAVPQL
       190        200        210        220        230        240
PSSDAFAAVA QLFQTTQGQQ LQQILQTFQQ PPKPQSPALD NAVMAQVQAI TAQLKTTPTQ
       250        260        270        280        290        300
PSEQKAAFPP PEQKTAFDKK LLDRFDYDDE PEAVEESKKE DTTAVTTTAP AAAVPPAPTA
       310        320        330        340        350        360
TVPAAAAPAA ASPPPPQAPF GFPGDGMQQP AYTQHQNMDQ FQPRMMGIQQ DPMHHQVPLP
       370        380        390        400        410        420
PNGQMPGFGL LPTPPFPPMA QPVIPPTPPV QQPFQASFQA QNEPLTQKPH QQEMEVEQPC
       430        440        450        460        470        480
IQEVKRHMSD NRKSRSRSAS RSPKRRRSRS GSRSRRSRHR RSRSRSRDRR RHSPRSRSQE
       490        500        510        520        530        540
RRDREKERER RQKGLPQVKP ETASVCSTTL WVGQLDKRTT QQDVASLLEE FGPIESINMI
       550        560        570        580        590        600
PPRGCAYIVM VHRQDAYRAL QKLSRGNYKV NQKSIKIAWA LNKGIKADYK QYWDVELGVT
       610        620        630        640        650        660
YIPWDKVKPE ELESFCEGGM LDSDTLNPDW KGIPKKPENE VAQNGGAETS HTEPVSPIPK
       670        680        690        700        710        720
PLPVPVPPIP VPAPITVPPP QVPPHQPGPP VVGALQPPAF TPPLGIPPPG FGPGVPPPPP
       730        740        750        760        770        780
PPPFLRPGFN PMHLPPGFLP PGPPPPITPP VSIPPPHTPP ISIPNSTIAG INEDTTKDLS
       790        800        810        820        830        840
IGNPIPTVVS GARGNAESGD SVKMYGSAVP PAAPTNLPTP PVTQPVSLLG TQGVAPGPVI
       850        860        870        880        890        900
GLQAPSTGLL GARPGLIPLQ RPPGMPPPHL QRFPLMPPRP MPPHMMHRGP PPGPGGFAMP
       910        920        930        940        950        960
PPHGMKGPFP PHGPFVRPGG MPGLGGPGPG PGGPEDRDGR QQPPQQPQQQ PQPQAPQQPQ
       970        980        990       1000       1010       1020
QQQQQQPPPS QQPPPTQQQP QQFRNDNRQQ FNSGRDQERF GRRSFGNRVE NDRERYGNRN
      1030       1040       1050       1060       1070       1080
DDRDNSNRDR REWGRRSPDR DRHRDLEERN RRSSGHRDRE RDSRDRESRR EKEEARGKEK
      1090       1100       1110       1120       1130       1140
PEVTDRAGGN KTVEPPISQV GNVDTASELE KGVSEAAVLK PSEELPAEAT SSVEPEKDSG

SAAEAPR         10         20         30         40         50         60
MDAVNAFNQE LFSLMDMKPP ISRAKMILIT KAAIKAIKLY KHVVQIVEKF IKKCKPEYKV
        70         80         90        100        110        120
PGLYVIDSIV RQSRHQFGTD KDVFGPRFSK NITATFQYLY LCPSEDKSKI VRVLNLWQKN
       130        140        150        160        170        180
GVFKIEIIQP LLDMAAGTSN AAPVAENVTN NEGSPPPPVK VSSEPPTQAT PNSVPAVPQL
       190        200        210        220        230        240
PSSDAFAAVA QLFQTTQGQQ LQQILQTFQQ PPKPQSPALD NAVMAQVQAI TAQLKTTPTQ
       250        260        270        280        290        300
PSEQKAAFPP PEQKTAFDKK LLDRFDYDDE PEAVEESKKE DTTAVTTTAP AAAVPPAPTA
       310        320        330        340        350        360
TVPAAAAPAA ASPPPPQAPF GFPGDGMQQP AYTQHQNMDQ FQPRMMGIQQ DPMHHQVPLP
       370        380        390        400        410        420
PNGQMPGFGL LPTPPFPPMA QPVIPPTPPV QQPFQASFQA QNEPLTQKPH QQEMEVEQPC
       430        440        450        460        470        480
IQEVKRHMSD NRKSRSRSAS RSPKRRRSRS GSRSRRSRHR RSRSRSRDRR RHSPRSRSQE
       490        500        510        520        530        540
RRDREKERER RQKGLPQVKP ETASVCSTTL WVGQLDKRTT QQDVASLLEE FGPIESINMI
       550        560        570        580        590        600
PPRGCAYIVM VHRQDAYRAL QKLSRGNYKV NQKSIKIAWA LNKGIKADYK QYWDVELGVT
       610        620        630        640        650        660
YIPWDKVKPE ELESFCEGGM LDSDTLNPDW KGIPKKPENE VAQNGGAETS HTEPVSPIPK
       670        680        690        700        710        720
PLPVPVPPIP VPAPITVPPP QVPPHQPGPP VVGALQPPAF TPPLGIPPPG FGPGVPPPPP
       730        740        750        760        770        780
PPPFLRPGFN PMHLPPGFLP PGPPPPITPP VSIPPPHTPP ISIPNSTIAG INEDTTKDLS
       790        800        810        820        830        840
IGNPIPTVVS GARGNAESGD SVKMYGSAVP PAAPTNLPTP PVTQPVSLLG TQGVAPGPVI
       850        860        870        880        890        900
GLQAPSTGLL GARPGLIPLQ RPPGMPPPHL QRFPLMPPRP MPPHMMHRGP PPGPGGFAMP
       910        920        930        940        950        960
PPHGMKGPFP PHGPFVRPGG MPGLGGPGPG PGGPEDRDGR QQPPQQPQQQ PQPQAPQQPQ
       970        980        990       1000       1010       1020
QQQQQQPPPS QQPPPTQQQP QQFRNDNRQQ FNSGRDQERF GRRSFGNRVE NDRERYGNRN
      1030       1040       1050       1060       1070       1080
DDRDNSNRDR REWGRRSPDR DRHRDLEERN RRSSGHRDRE RDSRDRESRR EKEEARGKEK
      1090       1100       1110       1120       1130       1140
PEVTDRAGGN KTVEPPISQV GNVDTASELE KGVSEAAVLK PSEELPAEAT SSVEPEKDSG

SAAEAPR         10         20         30         40         50         60
MDAVNAFNQE LFSLMDMKPP ISRAKMILIT KAAIKAIKLY KHVVQIVEKF IKKCKPEYKV
        70         80         90        100        110        120
PGLYVIDSIV RQSRHQFGTD KDVFGPRFSK NITATFQYLY LCPSEDKSKI VRVLNLWQKN
       130        140        150        160        170        180
GVFKIEIIQP LLDMAAGTSN AAPVAENVTN NEGSPPPPVK VSSEPPTQAT PNSVPAVPQL
       190        200        210        220        230        240
PSSDAFAAVA QLFQTTQGQQ LQQILQTFQQ PPKPQSPALD NAVMAQVQAI TAQLKTTPTQ
       250        260        270        280        290        300
PSEQKAAFPP PEQKTAFDKK LLDRFDYDDE PEAVEESKKE DTTAVTTTAP AAAVPPAPTA
       310        320        330        340        350        360
TVPAAAAPAA ASPPPPQAPF GFPGDGMQQP AYTQHQNMDQ FQPRMMGIQQ DPMHHQVPLP
       370        380        390        400        410        420
PNGQMPGFGL LPTPPFPPMA QPVIPPTPPV QQPFQASFQA QNEPLTQKPH QQEMEVEQPC
       430        440        450        460        470        480
IQEVKRHMSD NRKSRSRSAS RSPKRRRSRS GSRSRRSRHR RSRSRSRDRR RHSPRSRSQE
       490        500        510        520        530        540
RRDREKERER RQKGLPQVKP ETASVCSTTL WVGQLDKRTT QQDVASLLEE FGPIESINMI
       550        560        570        580        590        600
PPRGCAYIVM VHRQDAYRAL QKLSRGNYKV NQKSIKIAWA LNKGIKADYK QYWDVELGVT
       610        620        630        640        650        660
YIPWDKVKPE ELESFCEGGM LDSDTLNPDW KGIPKKPENE VAQNGGAETS HTEPVSPIPK
       670        680        690        700        710        720
PLPVPVPPIP VPAPITVPPP QVPPHQPGPP VVGALQPPAF TPPLGIPPPG FGPGVPPPPP
       730        740        750        760        770        780
PPPFLRPGFN PMHLPPGFLP PGPPPPITPP VSIPPPHTPP ISIPNSTIAG INEDTTKDLS
       790        800        810        820        830        840
IGNPIPTVVS GARGNAESGD SVKMYGSAVP PAAPTNLPTP PVTQPVSLLG TQGVAPGPVI
       850        860        870        880        890        900
GLQAPSTGLL GARPGLIPLQ RPPGMPPPHL QRFPLMPPRP MPPHMMHRGP PPGPGGFAMP
       910        920        930        940        950        960
PPHGMKGPFP PHGPFVRPGG MPGLGGPGPG PGGPEDRDGR QQPPQQPQQQ PQPQAPQQPQ
       970        980        990       1000       1010       1020
QQQQQQPPPS QQPPPTQQQP QQFRNDNRQQ FNSGRDQERF GRRSFGNRVE NDRERYGNRN
      1030       1040       1050       1060       1070       1080
DDRDNSNRDR REWGRRSPDR DRHRDLEERN RRSSGHRDRE RDSRDRESRR EKEEARGKEK
      1090       1100       1110       1120       1130       1140
PEVTDRAGGN KTVEPPISQV GNVDTASELE KGVSEAAVLK PSEELPAEAT SSVEPEKDSG

SAAEAPR         10         20         30         40         50         60
MDAVNAFNQE LFSLMDMKPP ISRAKMILIT KAAIKAIKLY KHVVQIVEKF IKKCKPEYKV
        70         80         90        100        110        120
PGLYVIDSIV RQSRHQFGTD KDVFGPRFSK NITATFQYLY LCPSEDKSKI VRVLNLWQKN
       130        140        150        160        170        180
GVFKIEIIQP LLDMAAGTSN AAPVAENVTN NEGSPPPPVK VSSEPPTQAT PNSVPAVPQL
       190        200        210        220        230        240
PSSDAFAAVA QLFQTTQGQQ LQQILQTFQQ PPKPQSPALD NAVMAQVQAI TAQLKTTPTQ
       250        260        270        280        290        300
PSEQKAAFPP PEQKTAFDKK LLDRFDYDDE PEAVEESKKE DTTAVTTTAP AAAVPPAPTA
       310        320        330        340        350        360
TVPAAAAPAA ASPPPPQAPF GFPGDGMQQP AYTQHQNMDQ FQPRMMGIQQ DPMHHQVPLP
       370        380        390        400        410        420
PNGQMPGFGL LPTPPFPPMA QPVIPPTPPV QQPFQASFQA QNEPLTQKPH QQEMEVEQPC
       430        440        450        460        470        480
IQEVKRHMSD NRKSRSRSAS RSPKRRRSRS GSRSRRSRHR RSRSRSRDRR RHSPRSRSQE
       490        500        510        520        530        540
RRDREKERER RQKGLPQVKP ETASVCSTTL WVGQLDKRTT QQDVASLLEE FGPIESINMI
       550        560        570        580        590        600
PPRGCAYIVM VHRQDAYRAL QKLSRGNYKV NQKSIKIAWA LNKGIKADYK QYWDVELGVT
       610        620        630        640        650        660
YIPWDKVKPE ELESFCEGGM LDSDTLNPDW KGIPKKPENE VAQNGGAETS HTEPVSPIPK
       670        680        690        700        710        720
PLPVPVPPIP VPAPITVPPP QVPPHQPGPP VVGALQPPAF TPPLGIPPPG FGPGVPPPPP
       730        740        750        760        770        780
PPPFLRPGFN PMHLPPGFLP PGPPPPITPP VSIPPPHTPP ISIPNSTIAG INEDTTKDLS
       790        800        810        820        830        840
IGNPIPTVVS GARGNAESGD SVKMYGSAVP PAAPTNLPTP PVTQPVSLLG TQGVAPGPVI
       850        860        870        880        890        900
GLQAPSTGLL GARPGLIPLQ RPPGMPPPHL QRFPLMPPRP MPPHMMHRGP PPGPGGFAMP
       910        920        930        940        950        960
PPHGMKGPFP PHGPFVRPGG MPGLGGPGPG PGGPEDRDGR QQPPQQPQQQ PQPQAPQQPQ
       970        980        990       1000       1010       1020
QQQQQQPPPS QQPPPTQQQP QQFRNDNRQQ FNSGRDQERF GRRSFGNRVE NDRERYGNRN
      1030       1040       1050       1060       1070       1080
DDRDNSNRDR REWGRRSPDR DRHRDLEERN RRSSGHRDRE RDSRDRESRR EKEEARGKEK
      1090       1100       1110       1120       1130       1140
PEVTDRAGGN KTVEPPISQV GNVDTASELE KGVSEAAVLK PSEELPAEAT SSVEPEKDSG

SAAEAPR

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

9 N-termini - 4 C-termini - 3 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
O95104-1-unknown	MDAVNA...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
O95104-1-unknown	MDAVNA...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt89404
O95104-1-unknown	MDAVNA...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt89405
O95104-1-acetylation	MDAVNA...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
O95104-1-acetylation	MDAVNA...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt163003
O95104-1-acetylation	MDAVNA...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt163004
O95104-354-unknown	HHQVPL...	354		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13864
O95104-354-unknown	HHQVPL...	354		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt150697
O95104-354-unknown	HHQVPL...	354		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt151027
O95104-406-unknown	TQKPHQ...	406		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13867
O95104-406-unknown	TQKPHQ...	406		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt151850
O95104-406-unknown	TQKPHQ...	406		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt152081
O95104-775-unknown	TTKDLS...	775		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11001
O95104-775-unknown	TTKDLS...	775		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt156313
O95104-780-unknown	SIGNPI...	780		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt177116
O95104-780-	SIGNPI...	780			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
O95104-1092-unknown	TVEPPI...	1092		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175779
O95104-1092-unknown	TVEPPI...	1092		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175778
O95104-1092-	TVEPPI...	1092			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...QQDPMH	353	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC13864
...QQDPMH	353	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt134095
...QQDPMH	353	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt134430
...QNEPLT	405	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC13867
...QNEPLT	405	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt135256
...QNEPLT	405	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt135490
...GINEDT	774	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11001
...GINEDT	774	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt139785
...AEAPR	1147	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...AEAPR	1147	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt85022
...AEAPR	1147	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt85023

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAM_HUMAN	353	DPMH.\|.HHQV	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,
GRAM_HUMAN	405	EPLT.\|.TQKP	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,
GRAB_HUMAN	774	NEDT.\|.TTKD	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)