P11387: DNA topoisomerase 1

General Information

Protein names	- DNA topoisomerase 1 - 5.6.2.2 {ECO:0000255\|PROSITE-ProRule:PRU10130, ECO:0000269\|PubMed:14594810, ECO:0000269\|PubMed:16033260, ECO:0000269\|PubMed:2833744} - DNA topoisomerase I
Gene names	TOP1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P11387

9 N-termini

7 C-termini

8 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK EKDREKSKHS NSEHKDSEKK
        70         80         90        100        110        120
HKEKEKTKHK DGSSEKHKDK HKDRDKEKRK EEKVRASGDA KIKKEKENGF SSPPQIKDEP
       130        140        150        160        170        180
EDDGYFVPPK EDIKPLKRPR DEDDADYKPK KIKTEDTKKE KKRKLEEEED GKLKKPKNKD
       190        200        210        220        230        240
KDKKVPEPDN KKKKPKKEEE QKWKWWEEER YPEGIKWKFL EHKGPVFAPP YEPLPENVKF
       250        260        270        280        290        300
YYDGKVMKLS PKAEEVATFF AKMLDHEYTT KEIFRKNFFK DWRKEMTNEE KNIITNLSKC
       310        320        330        340        350        360
DFTQMSQYFK AQTEARKQMS KEEKLKIKEE NEKLLKEYGF CIMDNHKERI ANFKIEPPGL
       370        380        390        400        410        420
FRGRGNHPKM GMLKRRIMPE DIIINCSKDA KVPSPPPGHK WKEVRHDNKV TWLVSWTENI
       430        440        450        460        470        480
QGSIKYIMLN PSSRIKGEKD WQKYETARRL KKCVDKIRNQ YREDWKSKEM KVRQRAVALY
       490        500        510        520        530        540
FIDKLALRAG NEKEEGETAD TVGCCSLRVE HINLHPELDG QEYVVEFDFL GKDSIRYYNK
       550        560        570        580        590        600
VPVEKRVFKN LQLFMENKQP EDDLFDRLNT GILNKHLQDL MEGLTAKVFR TYNASITLQQ
       610        620        630        640        650        660
QLKELTAPDE NIPAKILSYN RANRAVAILC NHQRAPPKTF EKSMMNLQTK IDAKKEQLAD
       670        680        690        700        710        720
ARRDLKSAKA DAKVMKDAKT KKVVESKKKA VQRLEEQLMK LEVQATDREE NKQIALGTSK
       730        740        750        760
LNYLDPRITV AWCKKWGVPI EKIYNKTQRE KFAWAIDMAD EDYEF

Isoforms

Sequence View

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Protein Neighborhood

Domains & Features

9 N-termini - 7 C-termini - 8 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P11387-2-unknown	MSGDHL...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P11387-2-Acetylation	SGDHLH...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P11387-10-unknown	SQIEAD...	10		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3218
P11387-16-unknown	FRLNDS...	16		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11187
P11387-109-	GFSSPP...	109			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P11387-124-unknown	GYFVPP...	124		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1647
P11387-124-unknown	GYFVPP...	124		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2496
P11387-147-unknown	YKPKKI...	147		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1648
P11387-171-unknown	GKLKKP...	171		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1649
P11387-171-unknown	GKLKKP...	171		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2497
P11387-529-unknown	FLGKDS...	529		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11188

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...HLHNDS	9	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3218
...QIEADF	15	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11187
...EPEDDG	123	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC1647
...EPEDDG	123	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2496
...EDDADY	146	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC1648
...EEEEDG	170	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC1649
...EEEEDG	170	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2497
...VVEFDF	528	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11188
...EDYEF	765	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CASPE_HUMAN	9	HNDS.\|.SQIE	inferred from experiment	unknown	MEROPS		Merops CASPE_HUMAN -> TOP1_HUMAN @9
GRAB_HUMAN	15	EADF.\|.FRLN	inferred from experiment	unknown	MEROPS	Rosen A	Casciola-Rosen L et al.:Cleavage by granzyme B is stron... (S01.010)	10499920,
CASP3_HUMAN	123	EDDG.\|.GYFV	inferred from experiment	unknown	MEROPS	Liu R	Ju W et al.:Proteome-wide identification of... (C14.003)	17728405,
CASP6_HUMAN	123	EDDG.\|.GYFV	inferred from experiment	unknown	MEROPS	Earnshaw WC	Samejima K et al.:Caspase-mediated cleavage of DN... (C14.003)	9933635,
CASP3_HUMAN	146	DADY.\|.YKPK	inferred from experiment	unknown	MEROPS	Earnshaw WC	Samejima K et al.:Caspase-mediated cleavage of DN... (C14.003)	9933635,
CASP3_HUMAN	170	EEDG.\|.GKLK	inferred from experiment	unknown	MEROPS	Earnshaw WC	Samejima K et al.:Caspase-mediated cleavage of DN... (C14.003)	9933635,
CASP6_HUMAN	170	EEDG.\|.GKLK	inferred from experiment	unknown	MEROPS	Earnshaw WC	Samejima K et al.:Caspase-mediated cleavage of DN... (C14.003)	9933635,
GRAB_HUMAN	528	EFDF.\|.FLGK	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)