P12814: Alpha-actinin-1

General Information

Protein names	- Alpha-actinin-1 - Alpha-actinin cytoskeletal isoform - F-actin cross-linking protein - Non-muscle alpha-actinin-1
Gene names	ACTN1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P12814

11 N-termini

9 C-termini

8 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform 2 of Alpha-actinin-1 - Isoform 3 of Alpha-actinin-1 - Isoform 4 of Alpha-actinin-1

Sequence View

        10         20         30         40         50         60
MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT QIENIEEDFR
        70         80         90        100        110        120
DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI ASKGVKLVSI GAEEIVDGNV
       130        140        150        160        170        180
KMTLGMIWTI ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC
       190        200        210        220        230        240
ALIHRHRPEL IDYGKLRKDD PLTNLNTAFD VAEKYLDIPK MLDAEDIVGT ARPDEKAIMT
       250        260        270        280        290        300
YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR
       310        320        330        340        350        360
VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR PAFMPSEGRM
       370        380        390        400        410        420
VSDINNAWGC LEQVEKGYEE WLLNEIRRLE RLDHLAEKFR QKASIHEAWT DGKEAMLRQK
       430        440        450        460        470        480
DYETATLSEI KALLKKHEAF ESDLAAHQDR VEQIAAIAQE LNELDYYDSP SVNARCQKIC
       490        500        510        520        530        540
DQWDNLGALT QKRREALERT EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT
       550        560        570        580        590        600
IEEIQGLTTA HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI
       610        620        630        640        650        660
NGKWDHVRQL VPRRDQALTE EHARQQHNER LRKQFGAQAN VIGPWIQTKM EEIGRISIEM
       670        680        690        700        710        720
HGTLEDQLSH LRQYEKSIVN YKPKIDQLEG DHQLIQEALI FDNKHTNYTM EHIRVGWEQL
       730        740        750        760        770        780
LTTIARTINE VENQILTRDA KGISQEQMNE FRASFNHFDR DHSGTLGPEE FKACLISLGY
       790        800        810        820        830        840
DIGNDPQGEA EFARIMSIVD PNRLGVVTFQ AFIDFMSRET ADTDTADQVM ASFKILAGDK
       850        860        870        880        890
NYITMDELRR ELPPDQAEYC IARMAPYTGP DSVPGALDYM SFSTALYGES DL         10         20         30         40         50         60
MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT QIENIEEDFR
        70         80         90        100        110        120
DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI ASKGVKLVSI GAEEIVDGNV
       130        140        150        160        170        180
KMTLGMIWTI ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC
       190        200        210        220        230        240
ALIHRHRPEL IDYGKLRKDD PLTNLNTAFD VAEKYLDIPK MLDAEDIVGT ARPDEKAIMT
       250        260        270        280        290        300
YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR
       310        320        330        340        350        360
VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR PAFMPSEGRM
       370        380        390        400        410        420
VSDINNAWGC LEQVEKGYEE WLLNEIRRLE RLDHLAEKFR QKASIHEAWT DGKEAMLRQK
       430        440        450        460        470        480
DYETATLSEI KALLKKHEAF ESDLAAHQDR VEQIAAIAQE LNELDYYDSP SVNARCQKIC
       490        500        510        520        530        540
DQWDNLGALT QKRREALERT EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT
       550        560        570        580        590        600
IEEIQGLTTA HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI
       610        620        630        640        650        660
NGKWDHVRQL VPRRDQALTE EHARQQHNER LRKQFGAQAN VIGPWIQTKM EEIGRISIEM
       670        680        690        700        710        720
HGTLEDQLSH LRQYEKSIVN YKPKIDQLEG DHQLIQEALI FDNKHTNYTM EHIRVGWEQL
       730        740        750        760        770        780
LTTIARTINE VENQILTRDA KGISQEQMNE FRASFNHFDR DHSGTLGPEE FKACLISLGY
       790        800        810        820        830        840
DIGNDPQGEA EFARIMSIVD PNRLGVVTFQ AFIDFMSRET ADTDTADQVM ASFKILAGDK
       850        860        870        880        890
NYITMDELRR ELPPDQAEYC IARMAPYTGP DSVPGALDYM SFSTALYGES DL         10         20         30         40         50         60
MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT QIENIEEDFR
        70         80         90        100        110        120
DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI ASKGVKLVSI GAEEIVDGNV
       130        140        150        160        170        180
KMTLGMIWTI ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC
       190        200        210        220        230        240
ALIHRHRPEL IDYGKLRKDD PLTNLNTAFD VAEKYLDIPK MLDAEDIVGT ARPDEKAIMT
       250        260        270        280        290        300
YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR
       310        320        330        340        350        360
VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR PAFMPSEGRM
       370        380        390        400        410        420
VSDINNAWGC LEQVEKGYEE WLLNEIRRLE RLDHLAEKFR QKASIHEAWT DGKEAMLRQK
       430        440        450        460        470        480
DYETATLSEI KALLKKHEAF ESDLAAHQDR VEQIAAIAQE LNELDYYDSP SVNARCQKIC
       490        500        510        520        530        540
DQWDNLGALT QKRREALERT EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT
       550        560        570        580        590        600
IEEIQGLTTA HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI
       610        620        630        640        650        660
NGKWDHVRQL VPRRDQALTE EHARQQHNER LRKQFGAQAN VIGPWIQTKM EEIGRISIEM
       670        680        690        700        710        720
HGTLEDQLSH LRQYEKSIVN YKPKIDQLEG DHQLIQEALI FDNKHTNYTM EHIRVGWEQL
       730        740        750        760        770        780
LTTIARTINE VENQILTRDA KGISQEQMNE FRASFNHFDR DHSGTLGPEE FKACLISLGY
       790        800        810        820        830        840
DIGNDPQGEA EFARIMSIVD PNRLGVVTFQ AFIDFMSRET ADTDTADQVM ASFKILAGDK
       850        860        870        880        890
NYITMDELRR ELPPDQAEYC IARMAPYTGP DSVPGALDYM SFSTALYGES DL

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

11 N-termini - 9 C-termini - 8 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P12814-1-Acetylation	MDHYDS...	1	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P12814-1-Acetylation	MDHYDS...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P12814-1-Acetylation	MDHYDS...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt106002
P12814-1-Acetylation	MDHYDS...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt106003
P12814-1-Acetylation	MDHYDS...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt106004
P12814-1-unknown	MDHYDS...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt66233
P12814-1-unknown	MDHYDS...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt66234
P12814-1-unknown	MDHYDS...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt66235
P12814-6-	SQQTND...	6			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P12814-6-	SQQTND...	6			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P12814-6-	SQQTND...	6			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P12814-6-	SQQTND...	6			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P12814-6-unknown	SQQTND...	6		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168625
P12814-6-unknown	SQQTND...	6		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168626
P12814-6-unknown	SQQTND...	6		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168627
P12814-19-unknown	WDRDLL...	19		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2498
P12814-19-unknown	WDRDLL...	19		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt136797
P12814-19-unknown	WDRDLL...	19		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt136798
P12814-19-unknown	WDRDLL...	19		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt136799
P12814-21-unknown	RDLLLD...	21		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2502
P12814-21-unknown	RDLLLD...	21		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt136942
P12814-21-unknown	RDLLLD...	21		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt136943
P12814-21-unknown	RDLLLD...	21		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt136944
P12814-59-unknown	FRDGLK...	59		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2506
P12814-59-unknown	FRDGLK...	59		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt139844
P12814-59-unknown	FRDGLK...	59		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt139845
P12814-59-unknown	FRDGLK...	59		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt139846
P12814-444-unknown	LAAHQD...	444		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2510
P12814-444-unknown	LAAHQD...	444		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt152705
P12814-444-unknown	LAAHQD...	444		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt152706
P12814-444-unknown	LAAHQD...	444		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt152707
P12814-466-unknown	YYDSPS...	466		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2514
P12814-466-unknown	YYDSPS...	466		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt153025
P12814-466-unknown	YYDSPS...	466		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt153026
P12814-466-unknown	YYDSPS...	466		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt153027
P12814-485-unknown	NLGALT...	485		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2518
P12814-485-unknown	NLGALT...	485		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt153255
P12814-485-unknown	NLGALT...	485		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt153256
P12814-485-unknown	NLGALT...	485		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt153257
P12814-692-unknown	HQLIQE...	692		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2522
P12814-692-unknown	HQLIQE...	692		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt155785
P12814-692-unknown	HQLIQE...	692		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt155786
P12814-692-unknown	HQLIQE...	692		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt155787

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...QPEEDW	18	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2498
...EEDWDR	20	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2502
...NIEEDF	58	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2506
...NIEEDF	58	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt122943
...NIEEDF	58	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt122944
...NIEEDF	58	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt122945
...AFESDL	443	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2510
...AFESDL	443	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt136125
...AFESDL	443	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt136126
...AFESDL	443	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt136127
...LNELDY	465	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2514
...LNELDY	465	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt136451
...LNELDY	465	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt136452
...LNELDY	465	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt136453
...CDQWDN	484	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2518
...CDQWDN	484	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt136679
...CDQWDN	484	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt136680
...CDQWDN	484	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt136681
...QLEGDH	691	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2522
...QLEGDH	691	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt139235
...QLEGDH	691	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt139236
...QLEGDH	691	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt139237
...YGESDL	891	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2526
...YGESDL	891	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt140301
...YGESDL	891	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt140439
...YGESDL	891	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt140500
...GESDL	892	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...GESDL	892	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt61851
...GESDL	892	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt61852
...GESDL	892	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt61853

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CASP6_HUMAN	18	EEDW.\|.WDRD	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CASP6_HUMAN	20	DWDR.\|.RDLL	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CASP6_HUMAN	58	EEDF.\|.FRDG	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CASP6_HUMAN	443	ESDL.\|.LAAH	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CASP6_HUMAN	465	ELDY.\|.YYDS	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CASP6_HUMAN	484	QWDN.\|.NLGA	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CASP6_HUMAN	691	EGDH.\|.HQLI	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CASP6_HUMAN	891	ESDL.\|.L	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)