P13995: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

General Information

Protein names	- Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial - NAD-dependent methylenetetrahydrofolate dehydrogenase {ECO:0000303\|PubMed:16100107} - 1.5.1.15 - Methenyltetrahydrofolate cyclohydrolase - 3.5.4.9
Gene names	MTHFD2
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P13995

8 N-termini

3 C-termini

1 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MAATSLMSAL AARLLQPAHS CSLRLRPFHL AAVRNEAVVI SGRKLAQQIK QEVRQEVEEW
        70         80         90        100        110        120
VASGNKRPHL SVILVGENPA SHSYVLNKTR AAAVVGINSE TIMKPASISE EELLNLINKL
       130        140        150        160        170        180
NNDDNVDGLL VQLPLPEHID ERRICNAVSP DKDVDGFHVI NVGRMCLDQY SMLPATPWGV
       190        200        210        220        230        240
WEIIKRTGIP TLGKNVVVAG RSKNVGMPIA MLLHTDGAHE RPGGDATVTI SHRYTPKEQL
       250        260        270        280        290        300
KKHTILADIV ISAAGIPNLI TADMIKEGAA VIDVGINRVH DPVTAKPKLV GDVDFEGVRQ
       310        320        330        340        350
KAGYITPVPG GVGPMTVAML MKNTIIAAKK VLRLEEREVL KSKELGVATN

Isoforms

- Isoform 2 of Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

Sequence View

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Protein Neighborhood

Domains & Features

8 N-termini - 3 C-termini - 1 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P13995-7-unknown	MSALAA...	7	inferred from electronic annotation	unknown	TISdb		inferred from TISdb
P13995-36-unknown	EAVVIS...	36	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P13995-36-unknown	EAVVIS...	36	inferred from similarity	unknown	UniProtKB		inferred from uniprot (by similarity)
P13995-103-unknown	MKPASI...	103	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000264090
P13995-128-	GLLVQL...	128		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P13995-128-	GLLVQL...	128		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13995-128-	GLLVQL...	128		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P13995-128-	GLLVQL...	128		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P13995-128-	GLLVQL...	128		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P13995-147-	AVSPDK...	147		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P13995-165-unknown	MCLDQY...	165	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000377614
P13995-213-	LHTDGA...	213		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P13995-264-unknown	MIKEGA...	264	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11211

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...LITADM	263	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11211
...GVATN	350		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...GVATN	350		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...GVATN	350	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...GVATN	350		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...GVATN	350		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAB_HUMAN	263	TADM.\|.MIKE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)