P27694: Replication protein A 70 kDa DNA-binding subunit

General Information

Protein names	- Replication protein A 70 kDa DNA-binding subunit - RP-A p70 - Replication factor A protein 1 - RF-A protein 1 - Single-stranded DNA-binding protein - Replication protein A 70 kDa DNA-binding subunit, N-terminally processed
Gene names	RPA1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P27694

15 N-termini

5 C-termini

2 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MVGQLSEGAI AAIMQKGDTN IKPILQVINI RPITTGNSPP RYRLLMSDGL NTLSSFMLAT
        70         80         90        100        110        120
QLNPLVEEEQ LSSNCVCQIH RFIVNTLKDG RRVVILMELE VLKSAEAVGV KIGNPVPYNE
       130        140        150        160        170        180
GLGQPQVAPP APAASPAASS RPQPQNGSSG MGSTVSKAYG ASKTFGKAAG PSLSHTSGGT
       190        200        210        220        230        240
QSKVVPIASL TPYQSKWTIC ARVTNKSQIR TWSNSRGEGK LFSLELVDES GEIRATAFNE
       250        260        270        280        290        300
QVDKFFPLIE VNKVYYFSKG TLKIANKQFT AVKNDYEMTF NNETSVMPCE DDHHLPTVQF
       310        320        330        340        350        360
DFTGIDDLEN KSKDSLVDII GICKSYEDAT KITVRSNNRE VAKRNIYLMD TSGKVVTATL
       370        380        390        400        410        420
WGEDADKFDG SRQPVLAIKG ARVSDFGGRS LSVLSSSTII ANPDIPEAYK LRGWFDAEGQ
       430        440        450        460        470        480
ALDGVSISDL KSGGVGGSNT NWKTLYEVKS ENLGQGDKPD YFSSVATVVY LRKENCMYQA
       490        500        510        520        530        540
CPTQDCNKKV IDQQNGLYRC EKCDTEFPNF KYRMILSVNI ADFQENQWVT CFQESAEAIL
       550        560        570        580        590        600
GQNAAYLGEL KDKNEQAFEE VFQNANFRSF IFRVRVKVET YNDESRIKAT VMDVKPVDYR
       610
EYGRRLVMSI RRSALM

Isoforms

Sequence View

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Evidence Names:

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Protein Neighborhood

Domains & Features

15 N-termini - 5 C-termini - 2 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P27694-1-Acetylation	MVGQLS...	1	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P27694-1-Acetylation	MVGQLS...	1	acetylation-		other		CNRS/ISV	Large_scale_NTA_Human
P27694-1-unknown	MVGQLS...	1			unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P27694-2-Acetylation	MVGQLS...	1	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P27694-1-	MVGQLS...	1			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P27694-1-	MVGQLS...	1			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P27694-1-	MVGQLS...	1			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P27694-1-	MVGQLS...	1			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P27694-2-unknown	VGQLSE...	2		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P27694-2-	VGQLSE...	2			Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P27694-2-	VGQLSE...	2			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P27694-7-unknown	EGAIAA...	7		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18993
P27694-14-unknown	MQKGDT...	14		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000461733
P27694-14-unknown	MQKGDT...	14		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000459788
P27694-82-	FIVNTL...	82			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P27694-164-	TFGKAA...	164			Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P27694-164-	TFGKAA...	164			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P27694-166-	GKAAGP...	166			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P27694-168-	AAGPSL...	168			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P27694-168-	AAGPSL...	168			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P27694-168-	AAGPSL...	168			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P27694-188-unknown	ASLTPY...	188			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P27694-245-unknown	FFPLIE...	245		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000461466
P27694-542-unknown	QNAAYL...	542		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7396

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...VGQLSE	6	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18993
...AASPAA	137	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000461733
...SKTFGK	166	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000459788
...EAILGQ	541	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7396
...RSALM	616	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
MEP1B_HUMAN	6	QLSE.\|.EGAI	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATS_HUMAN	541	ILGQ.\|.QNAA	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)