P54578: Ubiquitin carboxyl-terminal hydrolase 14

General Information

Protein names	- Ubiquitin carboxyl-terminal hydrolase 14 - 3.4.19.12 - Deubiquitinating enzyme 14 - Ubiquitin thioesterase 14 - Ubiquitin-specific-processing protease 14
Gene names	USP14
Organism	Homo sapiens
Protease Family
Protease ID	C19.015
Chromosome location
UniProt ID	P54578

12 N-termini

4 C-termini

2 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform 2 of Ubiquitin carboxyl-terminal hydrolase 14 - Isoform 3 of Ubiquitin carboxyl-terminal hydrolase 14

Sequence View

        10         20         30         40         50         60
MPLYSVTVKW GKEKFEGVEL NTDEPPMVFK AQLFALTGVQ PARQKVMVKG GTLKDDDWGN
        70         80         90        100        110        120
IKIKNGMTLL MMGSADALPE EPSAKTVFVE DMTEEQLASA MELPCGLTNL GNTCYMNATV
       130        140        150        160        170        180
QCIRSVPELK DALKRYAGAL RASGEMASAQ YITAALRDLF DSMDKTSSSI PPIILLQFLH
       190        200        210        220        230        240
MAFPQFAEKG EQGQYLQQDA NECWIQMMRV LQQKLEAIED DSVKETDSSS ASAATPSKKK
       250        260        270        280        290        300
SLIDQFFGVE FETTMKCTES EEEEVTKGKE NQLQLSCFIN QEVKYLFTGL KLRLQEEITK
       310        320        330        340        350        360
QSPTLQRNAL YIKSSKISRL PAYLTIQMVR FFYKEKESVN AKVLKDVKFP LMLDMYELCT
       370        380        390        400        410        420
PELQEKMVSF RSKFKDLEDK KVNQQPNTSD KKSSPQKEVK YEPFSFADDI GSNNCGYYDL
       430        440        450        460        470        480
QAVLTHQGRS SSSGHYVSWV KRKQDEWIKF DDDKVSIVTP EDILRLSGGG DWHIAYVLLY
       490
GPRRVEIMEE ESEQ         10         20         30         40         50         60
MPLYSVTVKW GKEKFEGVEL NTDEPPMVFK AQLFALTGVQ PARQKVMVKG GTLKDDDWGN
        70         80         90        100        110        120
IKIKNGMTLL MMGSADALPE EPSAKTVFVE DMTEEQLASA MELPCGLTNL GNTCYMNATV
       130        140        150        160        170        180
QCIRSVPELK DALKRYAGAL RASGEMASAQ YITAALRDLF DSMDKTSSSI PPIILLQFLH
       190        200        210        220        230        240
MAFPQFAEKG EQGQYLQQDA NECWIQMMRV LQQKLEAIED DSVKETDSSS ASAATPSKKK
       250        260        270        280        290        300
SLIDQFFGVE FETTMKCTES EEEEVTKGKE NQLQLSCFIN QEVKYLFTGL KLRLQEEITK
       310        320        330        340        350        360
QSPTLQRNAL YIKSSKISRL PAYLTIQMVR FFYKEKESVN AKVLKDVKFP LMLDMYELCT
       370        380        390        400        410        420
PELQEKMVSF RSKFKDLEDK KVNQQPNTSD KKSSPQKEVK YEPFSFADDI GSNNCGYYDL
       430        440        450        460        470        480
QAVLTHQGRS SSSGHYVSWV KRKQDEWIKF DDDKVSIVTP EDILRLSGGG DWHIAYVLLY
       490
GPRRVEIMEE ESEQ

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

12 N-termini - 4 C-termini - 2 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P54578-1-unknown	MPLYSV...	1	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P54578-1-unknown	MPLYSV...	1	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt93636
P54578-2-unmodified	PLYSVT...	2		other		CNRS/ISV	Large_scale_NTA_Human
P54578-2-unknown	PLYSVT...	2	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt163297
P54578-77-	ALPEEP...	77		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P54578-113-unknown	TCYMNA...	113	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt182777
P54578-113-	TCYMNA...	113		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P54578-142-unknown	ASGEMA...	142	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt182779
P54578-142-	ASGEMA...	142		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P54578-222-	SVKETD...	222		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P54578-222-	SVKETD...	222		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P54578-222-unknown	SVKETD...	222	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11686
P54578-222-unknown	SVKETD...	222	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt146153
P54578-228-	SSSASA...	228		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P54578-228-	SSSASA...	228		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P54578-228-	SSSASA...	228		Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P54578-228-unknown	SSSASA...	228	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11688
P54578-228-unknown	SSSASA...	228	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt146365

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...AIEDDS	221	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11686
...AIEDDS	221	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt129455
...VKETDS	227	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11688
...VKETDS	227	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt129669
...EESEQ	494	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...EESEQ	494	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt89254
...EESEQ	494		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...EESEQ	494		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...EESEQ	494		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAB_HUMAN	221	EDDS.\|.SVKE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	227	ETDS.\|.SSSA	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)