Q00403: Transcription initiation factor IIB {ECO:0000303|PubMed:1876184}

General Information

Protein names	- Transcription initiation factor IIB {ECO:0000303\|PubMed:1876184} - 2.3.1.48 {ECO:0000269\|PubMed:12931194} - General transcription factor TFIIB {ECO:0000303\|PubMed:1946368} - S300-II {ECO:0000303\|PubMed:1517211}
Gene names	GTF2B
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	Q00403

6 N-termini

4 C-termini

2 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA
        70         80         90        100        110        120
TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE
       130        140        150        160        170        180
ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI
       190        200        210        220        230        240
CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV
       250        260        270        280        290        300
ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF
       310
PTDFKFDTPV DKLPQL

Isoforms

Sequence View

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Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

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Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

6 N-termini - 4 C-termini - 2 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
Q00403-1-unknown	MASTSR...	1	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
Q00403-2-unknown	ASTSRL...	2	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000415741
Q00403-37-unknown	CGLVVG...	37	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11858
Q00403-54-unknown	TFSNDK...	54	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10171
Q00403-208-	LITTGD...	208		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
Q00403-208-	LITTGD...	208		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
Q00403-208-	LITTGD...	208		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q00403-208-	LITTGD...	208		Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
Q00403-208-unknown	LITTGD...	208		unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...MICPEC	36	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11858
...GSEWRT	53	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10171
...ATHIAR	236	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000402345
...KLPQL	316	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAB_HUMAN	36	CPEC.\|.CGLV	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,
GRAA_HUMAN	53	EWRT.\|.TFSN	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:The substrate specificity profi... (S01.135)	20536382,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)