Q15046: Lysine--tRNA ligase

General Information

Protein names	- Lysine--tRNA ligase - 2.7.7.- {ECO:0000269\|PubMed:19524539, ECO:0000269\|PubMed:23159739, ECO:0000269\|PubMed:5338216} - 6.1.1.6 {ECO:0000269\|PubMed:18029264, ECO:0000269\|PubMed:23159739, ECO:0000269\|PubMed:9278442} - Lysyl-tRNA synthetase - LysRS
Gene names	KARS
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	Q15046

9 N-termini

3 C-termini

2 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MAAVQAAEVK VDGSEPKLSK NELKRRLKAE KKVAEKEAKQ KELSEKQLSQ ATAAATNHTT
        70         80         90        100        110        120
DNGVGPEEES VDPNQYYKIR SQAIHQLKVN GEDPYPHKFH VDISLTDFIQ KYSHLQPGDH
       130        140        150        160        170        180
LTDITLKVAG RIHAKRASGG KLIFYDLRGE GVKLQVMANS RNYKSEEEFI HINNKLRRGD
       190        200        210        220        230        240
IIGVQGNPGK TKKGELSIIP YEITLLSPCL HMLPHLHFGL KDKETRYRQR YLDLILNDFV
       250        260        270        280        290        300
RQKFIIRSKI ITYIRSFLDE LGFLEIETPM MNIIPGGAVA KPFITYHNEL DMNLYMRIAP
       310        320        330        340        350        360
ELYHKMLVVG GIDRVYEIGR QFRNEGIDLT HNPEFTTCEF YMAYADYHDL MEITEKMVSG
       370        380        390        400        410        420
MVKHITGSYK VTYHPDGPEG QAYDVDFTPP FRRINMVEEL EKALGMKLPE TNLFETEETR
       430        440        450        460        470        480
KILDDICVAK AVECPPPRTT ARLLDKLVGE FLEVTCINPT FICDHPQIMS PLAKWHRSKE
       490        500        510        520        530        540
GLTERFELFV MKKEICNAYT ELNDPMRQRQ LFEEQAKAKA AGDDEAMFID ENFCTALEYG
       550        560        570        580        590
LPPTAGWGMG IDRVAMFLTD SNNIKEVLLF PAMKPEDKKE NVATTDTLES TTVGTSV

Isoforms

- Isoform Mitochondrial of Lysine--tRNA ligase

Sequence View

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

9 N-termini - 3 C-termini - 2 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
Q15046-2-unknown	MAAVQA...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
Q15046-2-Acetylation	AAVQAA...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
Q15046-2-Acetylation	AAVQAA...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
Q15046-2-Acetylation	AAVQAA...	2	acetylation-		other		CNRS/ISV	Large_scale_NTA_Human
Q15046-2-Acetylation	AAVQAA...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
Q15046-13-	GSEPKL...	13			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
Q15046-13-unknown	GSEPKL...	13		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2209
Q15046-63-	GVGPEE...	63			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
Q15046-63-	GVGPEE...	63			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q15046-63-	GVGPEE...	63			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q15046-63-	GVGPEE...	63			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
Q15046-63-	GVGPEE...	63			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
Q15046-63-	GVGPEE...	63			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
Q15046-63-unknown	GVGPEE...	63		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt166998
Q15046-68-unknown	EESVDP...	68		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC12341
Q15046-68-unknown	EESVDP...	68		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt142163
Q15046-498-unknown	AYTELN...	498		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt170704
Q15046-498-	AYTELN...	498			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...EVKVDG	12	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2209
...GVGPEE	67	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC12341
...GVGPEE	67	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt125350
...VGTSV	597	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...VGTSV	597	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt86804

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CASP3_HUMAN	12	KVDG.\|.GSEP	inferred from experiment	unknown	MEROPS		Merops CASP3_HUMAN -> SYK_HUMAN @12
GRAB_HUMAN	67	GPEE.\|.EESV	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)