Q92888: Rho guanine nucleotide exchange factor 1

General Information

Protein names	- Rho guanine nucleotide exchange factor 1 - 115 kDa guanine nucleotide exchange factor - p115-RhoGEF - p115RhoGEF - Sub1.5
Gene names	ARHGEF1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	Q92888

11 N-termini

2 C-termini

1 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform 2 of Rho guanine nucleotide exchange factor 1 - Isoform 3 of Rho guanine nucleotide exchange factor 1 - Isoform 4 of Rho guanine nucleotide exchange factor 1

Sequence View

        10         20         30         40         50         60
MEDFARGAAS PGPSRPGLVP VSIIGAEDED FENELETNSE EQNSQFQSLE QVKRRPAHLM
        70         80         90        100        110        120
ALLQHVALQF EPGPLLCCLH ADMLGSLGPK EAKKAFLDFY HSFLEKTAVL RVPVPPNVAF
       130        140        150        160        170        180
ELDRTRADLI SEDVQRRFVQ EVVQSQQVAV GRQLEDFRSK RLMGMTPWEQ ELAQLEAWVG
       190        200        210        220        230        240
RDRASYEARE RHVAERLLMH LEEMQHTIST DEEKSAAVVN AIGLYMRHLG VRTKSGDKKS
       250        260        270        280        290        300
GRNFFRKKVM GNRRSDEPAK TKKGLSSILD AARWNRGEPQ VPDFRHLKAE VDAEKPGATD
       310        320        330        340        350        360
RKGGVGMPSR DRNIGAPGQD TPGVSLHPLS LDSPDREPGA DAPLELGDSS PQGPMSLESL
       370        380        390        400        410        420
APPESTDEGA ETESPEPGDE GEPGRSGLEL EPEEPPGWRE LVPPDTLHSL PKSQVKRQEV
       430        440        450        460        470        480
ISELLVTEAA HVRMLRVLHD LFFQPMAECL FFPLEELQNI FPSLDELIEV HSLFLDRLMK
       490        500        510        520        530        540
RRQESGYLIE EIGDVLLARF DGAEGSWFQK ISSRFCSRQS FALEQLKAKQ RKDPRFCAFV
       550        560        570        580        590        600
QEAESRPRCR RLQLKDMIPT EMQRLTKYPL LLQSIGQNTE EPTEREKVEL AAECCREILH
       610        620        630        640        650        660
HVNQAVRDME DLLRLKDYQR RLDLSHLRQS SDPMLSEFKN LDITKKKLVH EGPLTWRVTK
       670        680        690        700        710        720
DKAVEVHVLL LDDLLLLLQR QDERLLLKSH SRTLTPTPDG KTMLRPVLRL TSAMTREVAT
       730        740        750        760        770        780
DHKAFYVLFT WDQEAQIYEL VAQTVSERKN WCALITETAG SLKVPAPASR PKPRPSPSST
       790        800        810        820        830        840
REPLLSSSEN GNGGRETSPA DARTERILSD LLPFCRPGPE GQLAATALRK VLSLKQLLFP
       850        860        870        880        890        900
AEEDNGAGPP RDGDGVPGGG PLSPARTQEI QENLLSLEET MKQLEELEEE FCRLRPLLSQ
       910
LGGNSVPQPG CT         10         20         30         40         50         60
MEDFARGAAS PGPSRPGLVP VSIIGAEDED FENELETNSE EQNSQFQSLE QVKRRPAHLM
        70         80         90        100        110        120
ALLQHVALQF EPGPLLCCLH ADMLGSLGPK EAKKAFLDFY HSFLEKTAVL RVPVPPNVAF
       130        140        150        160        170        180
ELDRTRADLI SEDVQRRFVQ EVVQSQQVAV GRQLEDFRSK RLMGMTPWEQ ELAQLEAWVG
       190        200        210        220        230        240
RDRASYEARE RHVAERLLMH LEEMQHTIST DEEKSAAVVN AIGLYMRHLG VRTKSGDKKS
       250        260        270        280        290        300
GRNFFRKKVM GNRRSDEPAK TKKGLSSILD AARWNRGEPQ VPDFRHLKAE VDAEKPGATD
       310        320        330        340        350        360
RKGGVGMPSR DRNIGAPGQD TPGVSLHPLS LDSPDREPGA DAPLELGDSS PQGPMSLESL
       370        380        390        400        410        420
APPESTDEGA ETESPEPGDE GEPGRSGLEL EPEEPPGWRE LVPPDTLHSL PKSQVKRQEV
       430        440        450        460        470        480
ISELLVTEAA HVRMLRVLHD LFFQPMAECL FFPLEELQNI FPSLDELIEV HSLFLDRLMK
       490        500        510        520        530        540
RRQESGYLIE EIGDVLLARF DGAEGSWFQK ISSRFCSRQS FALEQLKAKQ RKDPRFCAFV
       550        560        570        580        590        600
QEAESRPRCR RLQLKDMIPT EMQRLTKYPL LLQSIGQNTE EPTEREKVEL AAECCREILH
       610        620        630        640        650        660
HVNQAVRDME DLLRLKDYQR RLDLSHLRQS SDPMLSEFKN LDITKKKLVH EGPLTWRVTK
       670        680        690        700        710        720
DKAVEVHVLL LDDLLLLLQR QDERLLLKSH SRTLTPTPDG KTMLRPVLRL TSAMTREVAT
       730        740        750        760        770        780
DHKAFYVLFT WDQEAQIYEL VAQTVSERKN WCALITETAG SLKVPAPASR PKPRPSPSST
       790        800        810        820        830        840
REPLLSSSEN GNGGRETSPA DARTERILSD LLPFCRPGPE GQLAATALRK VLSLKQLLFP
       850        860        870        880        890        900
AEEDNGAGPP RDGDGVPGGG PLSPARTQEI QENLLSLEET MKQLEELEEE FCRLRPLLSQ
       910
LGGNSVPQPG CT         10         20         30         40         50         60
MEDFARGAAS PGPSRPGLVP VSIIGAEDED FENELETNSE EQNSQFQSLE QVKRRPAHLM
        70         80         90        100        110        120
ALLQHVALQF EPGPLLCCLH ADMLGSLGPK EAKKAFLDFY HSFLEKTAVL RVPVPPNVAF
       130        140        150        160        170        180
ELDRTRADLI SEDVQRRFVQ EVVQSQQVAV GRQLEDFRSK RLMGMTPWEQ ELAQLEAWVG
       190        200        210        220        230        240
RDRASYEARE RHVAERLLMH LEEMQHTIST DEEKSAAVVN AIGLYMRHLG VRTKSGDKKS
       250        260        270        280        290        300
GRNFFRKKVM GNRRSDEPAK TKKGLSSILD AARWNRGEPQ VPDFRHLKAE VDAEKPGATD
       310        320        330        340        350        360
RKGGVGMPSR DRNIGAPGQD TPGVSLHPLS LDSPDREPGA DAPLELGDSS PQGPMSLESL
       370        380        390        400        410        420
APPESTDEGA ETESPEPGDE GEPGRSGLEL EPEEPPGWRE LVPPDTLHSL PKSQVKRQEV
       430        440        450        460        470        480
ISELLVTEAA HVRMLRVLHD LFFQPMAECL FFPLEELQNI FPSLDELIEV HSLFLDRLMK
       490        500        510        520        530        540
RRQESGYLIE EIGDVLLARF DGAEGSWFQK ISSRFCSRQS FALEQLKAKQ RKDPRFCAFV
       550        560        570        580        590        600
QEAESRPRCR RLQLKDMIPT EMQRLTKYPL LLQSIGQNTE EPTEREKVEL AAECCREILH
       610        620        630        640        650        660
HVNQAVRDME DLLRLKDYQR RLDLSHLRQS SDPMLSEFKN LDITKKKLVH EGPLTWRVTK
       670        680        690        700        710        720
DKAVEVHVLL LDDLLLLLQR QDERLLLKSH SRTLTPTPDG KTMLRPVLRL TSAMTREVAT
       730        740        750        760        770        780
DHKAFYVLFT WDQEAQIYEL VAQTVSERKN WCALITETAG SLKVPAPASR PKPRPSPSST
       790        800        810        820        830        840
REPLLSSSEN GNGGRETSPA DARTERILSD LLPFCRPGPE GQLAATALRK VLSLKQLLFP
       850        860        870        880        890        900
AEEDNGAGPP RDGDGVPGGG PLSPARTQEI QENLLSLEET MKQLEELEEE FCRLRPLLSQ
       910
LGGNSVPQPG CT

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

11 N-termini - 2 C-termini - 1 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
Q92888-1-acetylation	MEDFAR...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
Q92888-1-acetylation	MEDFAR...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
Q92888-1-acetylation	MEDFAR...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt162932
Q92888-1-acetylation	MEDFAR...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt162931
Q92888-1-unknown	MEDFAR...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
Q92888-1-unknown	MEDFAR...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt67374
Q92888-1-unknown	MEDFAR...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt98421
Q92888-263-unknown	KGLSSI...	263			unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
Q92888-263-unknown	KGLSSI...	263		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt159420
Q92888-263-unknown	KGLSSI...	263		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt159419
Q92888-263-	KGLSSI...	263			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
Q92888-286-unknown	HLKAEV...	286		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10460
Q92888-286-unknown	HLKAEV...	286		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt148563
Q92888-286-unknown	HLKAEV...	286		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt149905
Q92888-293-unknown	AEKPGA...	293			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
Q92888-293-unknown	AEKPGA...	293		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt161282
Q92888-293-unknown	AEKPGA...	293		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt161281
Q92888-293-	AEKPGA...	293			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
Q92888-359-	SLAPPE...	359			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q92888-359-	SLAPPE...	359			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
Q92888-359-unknown	SLAPPE...	359		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175888
Q92888-359-unknown	SLAPPE...	359		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175887
Q92888-539-unknown	FVQEAE...	539		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt171924
Q92888-539-unknown	FVQEAE...	539		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt171923
Q92888-539-	FVQEAE...	539			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...VPDFRH	285	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10460
...VPDFRH	285	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt131937
...VPDFRH	285	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt133297
...QPGCT	912	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...QPGCT	912	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt62992
...QPGCT	912	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt62993

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAA_HUMAN	285	DFRH.\|.HLKA	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:The substrate specificity profi... (S01.135)	20536382,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)