Q9NSE4: Isoleucine--tRNA ligase, mitochondrial

General Information

Protein names	- Isoleucine--tRNA ligase, mitochondrial - 6.1.1.5 - Isoleucyl-tRNA synthetase - IleRS
Gene names	IARS2
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	Q9NSE4

6 N-termini

4 C-termini

3 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MRWGLRPRGP GAAALATARS LWGTPRLPCS PGWQGATKRL LVRSVSGASN HQPNSNSGRY
        70         80         90        100        110        120
RDTVLLPQTS FPMKLLGRQQ PDTELEIQQK CGFSELYSWQ RERKVKTEFC LHDGPPYANG
       130        140        150        160        170        180
DPHVGHALNK ILKDIANRFH MMNGSKIHFV PGWDCHGLPI EIKVLSELGR EAQNLSAMEI
       190        200        210        220        230        240
RKKARSFAKA AIEKQKSAFI RWGIMADWNN CYYTFDGKYE AKQLRTFYQM YDKGLVYRSY
       250        260        270        280        290        300
KPVFWSPSSR TALAEAELEY NPEHVSRSIY VKFPLLKPSP KLASLIDGSS PVSILVWTTQ
       310        320        330        340        350        360
PWTIPANEAV CYMPESKYAV VKCSKSGDLY VLAADKVASV ASTLETTFET ISTLSGVDLE
       370        380        390        400        410        420
NGTCSHPLIP DKASPLLPAN HVTMAKGTGL VHTAPAHGME DYGVASQHNL PMDCLVDEDG
       430        440        450        460        470        480
VFTDVAGPEL QNKAVLEEGT DVVIKMLQTA KNLLKEEKLV HSYPYDWRTK KPVVIRASKQ
       490        500        510        520        530        540
WFINITDIKT AAKELLKKVK FIPGSALNGM VEMMDRRPYW CISRQRVWGV PIPVFHHKTK
       550        560        570        580        590        600
DEYLINSQTT EHIVKLVEQH GSDIWWTLPP EQLLPKEVLS EVGGPDALEY VPGQDILDIW
       610        620        630        640        650        660
FDSGTSWSYV LPGPDQRADL YLEGKDQLGG WFQSSLLTSV AARKRAPYKT VIVHGFTLGE
       670        680        690        700        710        720
KGEKMSKSLG NVIHPDVVVN GGQDQSKEPP YGADVLRWWV ADSNVFTEVA IGPSVLNAAR
       730        740        750        760        770        780
DDISKLRNTL RFLLGNVADF NPETDSIPVN DMYVIDQYML HLLQDLANKI TELYKQYDFG
       790        800        810        820        830        840
KVVRLLRTFY TRELSNFYFS IIKDRLYCEK ENDPKRRSCQ TALVEILDVI VRSFAPILPH
       850        860        870        880        890        900
LAEEVFQHIP YIKEPKSVFR TGWISTSSIW KKPGLEEAVE SACAMRDSFL GSIPGKNAAE
       910        920        930        940        950        960
YKVITVIEPG LLFEIIEMLQ SEETSSTSQL NELMMASEST LLAQEPREMT ADVIELKGKF
       970        980        990       1000       1010
LINLEGGDIR EESSYKVIVM PTTKEKCPRC WKYTAESSDT LCPRCAEVVS GK

Isoforms

Sequence View

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Protein Neighborhood

Domains & Features

6 N-termini - 4 C-termini - 3 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
Q9NSE4-45-unknown	VSGASN...	45		unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
Q9NSE4-45-unknown	VSGASN...	45		unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
Q9NSE4-45-	VSGASN...	45		Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
Q9NSE4-45-	VSGASN...	45		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
Q9NSE4-45-	VSGASN...	45		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
Q9NSE4-45-	VSGASN...	45		Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
Q9NSE4-45-	VSGASN...	45		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
Q9NSE4-45-	VSGASN...	45		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q9NSE4-45-	VSGASN...	45		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q9NSE4-45-	VSGASN...	45		Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
Q9NSE4-45-	VSGASN...	45		Subtiligase Based Positive Selection		Wells	RMPI_untreated	23264352
Q9NSE4-45-	VSGASN...	45		Subtiligase Based Positive Selection		Wells	THP_untreated	23264352
Q9NSE4-45-	VSGASN...	45		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
Q9NSE4-49-unknown	SNHQPN...	49	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
Q9NSE4-73-unknown	MKLLGR...	73	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000355889
Q9NSE4-260-unknown	YNPEHV...	260	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC8104
Q9NSE4-547-unknown	SQTTEH...	547	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6803
Q9NSE4-547-unknown	SQTTEH...	547	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC8105

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...EAELEY	259	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC8104
...EYLINS	546	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6803
...EYLINS	546	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC8105
...VVSGK	1012		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...VVSGK	1012		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...VVSGK	1012	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...VVSGK	1012		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...VVSGK	1012		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CATS_HUMAN	259	ELEY.\|.YNPE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	546	LINS.\|.SQTT	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	546	LINS.\|.SQTT	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)