O00273: DNA fragmentation factor subunit alpha

General Information

Protein names	- DNA fragmentation factor subunit alpha - DNA fragmentation factor 45 kDa subunit - DFF-45 - Inhibitor of CAD - ICAD
Gene names	DFFA
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	O00273

9 N-termini

5 C-termini

5 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT
        70         80         90        100        110        120
LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA
       130        140        150        160        170        180
GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD
       190        200        210        220        230        240
QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH
       250        260        270        280        290        300
ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE RELALRLQQT
       310        320        330
QSLHSLRSIS ASKASPPGDL QNPKRARQDP T

Isoforms

- Isoform DFF35 of DNA fragmentation factor subunit alpha

Sequence View

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Protein Neighborhood

Domains & Features

9 N-termini - 5 C-termini - 5 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
O00273-1-unknown	MEVTGD...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt72656
O00273-1-Acetylation	MEVTGD...	1	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
O00273-1-Acetylation	MEVTGD...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
O00273-1-Acetylation	MEVTGD...	1	acetylation-		other		CNRS/ISV	Large_scale_NTA_Human
O00273-1-Acetylation	MEVTGD...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
O00273-1-Acetylation	MEVTGD...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt108247
O00273-7-	AGVPES...	7			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O00273-7-unknown	AGVPES...	7		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10635
O00273-7-unknown	AGVPES...	7		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt171390
O00273-108-unknown	QESFDV...	108		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13691
O00273-108-unknown	QESFDV...	108		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt142829
O00273-118-unknown	SGAGLK...	118		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1385
O00273-118-unknown	SGAGLK...	118		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt143370
O00273-222-unknown	AVDTGI...	222		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt171392
O00273-222-	AVDTGI...	222			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O00273-225-unknown	TGISRE...	225		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1387
O00273-225-unknown	TGISRE...	225		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC12522
O00273-225-unknown	TGISRE...	225		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt147547

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...EVTGDA	6	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10635
...TAWISQ	107	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC13691
...TAWISQ	107	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt126035
...VDETDS	117	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC1385
...VDETDS	117	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt126602
...VDAVDT	224	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC1387
...VDAVDT	224	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC12522
...VDAVDT	224	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt130898
...RQDPT	331	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAB_HUMAN	6	TGDA.\|.AGVP	inferred from experiment	unknown	MEROPS		Merops GRAB_HUMAN -> DFFA_HUMAN @6
GRAB_HUMAN	6	TGDA.\|.AGVP	inferred from experiment	unknown	MEROPS	Gevaert K	Plasman K et al.:Probing the efficiency of prote... (S01.010)	21048194,
GRAM_HUMAN	107	WISQ.\|.QESF	inferred from experiment	unknown	MEROPS	Fan Z	Lu H et al.:Granzyme M directly cleaves inh... (S01.139)	16818775,
CASP3_HUMAN	117	ETDS.\|.SGAG	inferred from experiment	unknown	MEROPS	Wang X	Liu X et al.:DFF, a heterodimeric protein th... (C14.003)	9108473,
CASP3_HUMAN	224	AVDT.\|.TGIS	inferred from experiment	unknown	MEROPS	Wang X	Liu X et al.:DFF, a heterodimeric protein th... (C14.003)	9108473,
GRAB_HUMAN	224	AVDT.\|.TGIS	inferred from experiment	unknown	MEROPS	Sekaly RP	Sharif-Askari E et al.:Direct cleavage of the human DN... (S01.010)	11406587,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)