P10515: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

General Information

Protein names	- Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial - 2.3.1.12 - 70 kDa mitochondrial autoantigen of primary biliary cirrhosis - PBC - Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex - M2 antigen complex 70 kDa subunit - Pyruvate dehydrogenase complex component E2 - PDC-E2 - PDCE2
Gene names	DLAT
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P10515

10 N-termini

7 C-termini

9 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MWRVCARRAQ NVAPWAGLEA RWTALQEVPG TPRVTSRSGP APARRNSVTT GYGGVRALCG
        70         80         90        100        110        120
WTPSSGATPR NRLLLQLLGS PGRRYYSLPP HQKVPLPSLS PTMQAGTIAR WEKKEGDKIN
       130        140        150        160        170        180
EGDLIAEVET DKATVGFESL EECYMAKILV AEGTRDVPIG AIICITVGKP EDIEAFKNYT
       190        200        210        220        230        240
LDSSAAPTPQ AAPAPTPAAT ASPPTPSAQA PGSSYPPHMQ VLLPALSPTM TMGTVQRWEK
       250        260        270        280        290        300
KVGEKLSEGD LLAEIETDKA TIGFEVQEEG YLAKILVPEG TRDVPLGTPL CIIVEKEADI
       310        320        330        340        350        360
SAFADYRPTE VTDLKPQVPP PTPPPVAAVP PTPQPLAPTP SAPCPATPAG PKGRVFVSPL
       370        380        390        400        410        420
AKKLAVEKGI DLTQVKGTGP DGRITKKDID SFVPSKVAPA PAAVVPPTGP GMAPVPTGVF
       430        440        450        460        470        480
TDIPISNIRR VIAQRLMQSK QTIPHYYLSI DVNMGEVLLV RKELNKILEG RSKISVNDFI
       490        500        510        520        530        540
IKASALACLK VPEANSSWMD TVIRQNHVVD VSVAVSTPAG LITPIVFNAH IKGVETIAND
       550        560        570        580        590        600
VVSLATKARE GKLQPHEFQG GTFTISNLGM FGIKNFSAII NPPQACILAI GASEDKLVPA
       610        620        630        640
DNEKGFDVAS MMSVTLSCDH RVVDGAVGAQ WLAEFRKYLE KPITMLL

Isoforms

Sequence View

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Evidence Codes:

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Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

10 N-termini - 7 C-termini - 9 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P10515-42-unknown	PARRNS...	42	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000433432
P10515-85-	YYSLPP...	85		Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P10515-86-	YSLPPH...	86		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P10515-86-	YSLPPH...	86		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P10515-87-	SLPPHQ...	87		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P10515-87-	SLPPHQ...	87		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P10515-87-	SLPPHQ...	87		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P10515-87-	SLPPHQ...	87		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P10515-87-	SLPPHQ...	87		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P10515-87-	SLPPHQ...	87		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P10515-87-unknown	SLPPHQ...	87	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P10515-103-unknown	MQAGTI...	103	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000442427
P10515-132-unknown	KATVGF...	132	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1634
P10515-132-unknown	KATVGF...	132	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11165
P10515-132-unknown	KATVGF...	132	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2485
P10515-132-unknown	KATVGF...	132	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3041
P10515-259-unknown	KATIGF...	259	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1635
P10515-259-unknown	KATIGF...	259	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11166
P10515-259-unknown	KATIGF...	259	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2486
P10515-259-unknown	KATIGF...	259	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3042
P10515-382-unknown	GRITKK...	382	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11167
P10515-589-	AIGASE...	589		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...EVETDK	131	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1634
...EVETDK	131	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11165
...EVETDK	131	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2485
...EVETDK	131	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3041
...EIETDK	258	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1635
...EIETDK	258	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11166
...EIETDK	258	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2486
...EIETDK	258	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3042
...GTGPDG	381	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11167
...SKVAPA	399	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000459115
...DNEKGF	605	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000468802
...ITMLL	647		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...ITMLL	647		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...ITMLL	647	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...ITMLL	647		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...ITMLL	647		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CASP3_HUMAN	131	ETDK.\|.KATV	inferred from experiment	unknown	MEROPS	Gershwin ME	Matsumura S et al.:Contribution to antimitochondri... (C14.003)	11786955,
GRAB_HUMAN	131	ETDK.\|.KATV	inferred from experiment	unknown	MEROPS	Gershwin ME	Matsumura S et al.:Contribution to antimitochondri... (C14.003)	11786955,
CASP6_HUMAN	131	ETDK.\|.KATV	inferred from experiment	unknown	MEROPS	Gershwin ME	Matsumura S et al.:Contribution to antimitochondri... (C14.003)	11786955,
CASP8_HUMAN	131	ETDK.\|.KATV	inferred from experiment	unknown	MEROPS	Gershwin ME	Matsumura S et al.:Contribution to antimitochondri... (C14.003)	11786955,
CASP3_HUMAN	258	ETDK.\|.KATI	inferred from experiment	unknown	MEROPS	Gershwin ME	Matsumura S et al.:Contribution to antimitochondri... (C14.003)	11786955,
GRAB_HUMAN	258	ETDK.\|.KATI	inferred from experiment	unknown	MEROPS	Gershwin ME	Matsumura S et al.:Contribution to antimitochondri... (C14.003)	11786955,
CASP6_HUMAN	258	ETDK.\|.KATI	inferred from experiment	unknown	MEROPS	Gershwin ME	Matsumura S et al.:Contribution to antimitochondri... (C14.003)	11786955,
CASP8_HUMAN	258	ETDK.\|.KATI	inferred from experiment	unknown	MEROPS	Gershwin ME	Matsumura S et al.:Contribution to antimitochondri... (C14.003)	11786955,
GRAB_HUMAN	381	GPDG.\|.GRIT	inferred from experiment	unknown	MEROPS	Gershwin ME	Matsumura S et al.:Contribution to antimitochondri... (C14.003)	11786955,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)