P18615: Negative elongation factor E

General Information

Protein names	- Negative elongation factor E - NELF-E - RNA-binding protein RD
Gene names	NELFE
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P18615

9 N-termini

8 C-termini

2 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform 2 of Negative elongation factor E - Isoform 3 of Negative elongation factor E

Sequence View

        10         20         30         40         50         60
MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL SEQPVMDTAT
        70         80         90        100        110        120
ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE KGPVPTFQPF QRSISADDDL
       130        140        150        160        170        180
QESSRRPQRK SLYESFVSSS DRLRELGPDG EEAEGPGAGD GPPRSFDWGY EERSGAHSSA
       190        200        210        220        230        240
SPPRSRSRDR SHERNRDRDR DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR
       250        260        270        280        290        300
DREGPFRRSD SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV
       310        320        330        340        350        360
TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV QNSPKGCHRD
       370        380
KRTQIVYSDD VYKENLVDGF         10         20         30         40         50         60
MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL SEQPVMDTAT
        70         80         90        100        110        120
ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE KGPVPTFQPF QRSISADDDL
       130        140        150        160        170        180
QESSRRPQRK SLYESFVSSS DRLRELGPDG EEAEGPGAGD GPPRSFDWGY EERSGAHSSA
       190        200        210        220        230        240
SPPRSRSRDR SHERNRDRDR DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR
       250        260        270        280        290        300
DREGPFRRSD SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV
       310        320        330        340        350        360
TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV QNSPKGCHRD
       370        380
KRTQIVYSDD VYKENLVDGF

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

9 N-termini - 8 C-termini - 2 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P18615-1-	MLVIPP...	1		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P18615-1-	MLVIPP...	1		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P18615-1-	MLVIPP...	1		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P18615-1-	MLVIPP...	1		Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
P18615-1-	MLVIPP...	1		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P18615-1-	MLVIPP...	1		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P18615-1-	MLVIPP...	1		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P18615-1-	MLVIPP...	1		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P18615-1-	MLVIPP...	1		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P18615-1-unknown	MLVIPP...	1	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P18615-1-unknown	MLVIPP...	1		unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P18615-27-unknown	KALLAL...	27	inferred from electronic annotation	unknown	TISdb		inferred from TISdb
P18615-49-	SLSEQP...	49		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P18615-113-unknown	SISADD...	113	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10022
P18615-113-	SISADD...	113		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P18615-131-	SLYESF...	131		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P18615-131-	SLYESF...	131		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P18615-131-unknown	SLYESF...	131		unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P18615-150-unknown	GEEAEG...	150	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11302

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...QPFQRS	112	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10022
...ELGPDG	149	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11302
...GPGAGD	159	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000414014
...GPGAGD	159	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000409139
...GPGAGD	159	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000390689
...GPGAGD	159	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000394340
...GPGAGD	159	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000411487
...GPGAGD	159	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000401342
...DREGPF	245	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000411724
...DREGPF	245	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000396971
...DREGPF	245	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000396604
...DREGPF	245	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000393793
...DREGPF	245	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000395175
...GNTLYV	265	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000414029
...LSMDPP	293	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000397565
...LSMDPP	293	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000410872
...LSMDPP	293	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000407630
...LSMDPP	293	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000409389
...LSMDPP	293	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000409269
...LSMDPP	293	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000393794
...PMLDAA	338	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000393103
...PMLDAA	338	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000407528
...PMLDAA	338	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000401745
...PMLDAA	338	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000397914
...PMLDAA	338	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000403630
...PMLDAA	338	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000415567
...LVDGF	380	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAA_HUMAN	112	FQRS.\|.SISA	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:The substrate specificity profi... (S01.135)	20536382,
GRAB_HUMAN	149	GPDG.\|.GEEA	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)