P27695: DNA-(apurinic or apyrimidinic site) lyase

General Information

Protein names	- DNA-(apurinic or apyrimidinic site) lyase - 3.1.-.- - 4.2.99.18 - APEX nuclease - APEN - Apurinic-apyrimidinic endonuclease 1 - AP endonuclease 1 - APE-1 - REF-1 - Redox factor-1 - DNA-(apurinic or apyrimidinic site) lyase, mitochondrial
Gene names	APEX1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P27695

10 N-termini

14 C-termini

4 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MPKRGKKGAV AEDGDELRTE PEAKKSKTAA KKNDKEAAGE GPALYEDPPD QKTSPSGKPA
        70         80         90        100        110        120
TLKICSWNVD GLRAWIKKKG LDWVKEEAPD ILCLQETKCS ENKLPAELQE LPGLSHQYWS
       130        140        150        160        170        180
APSDKEGYSG VGLLSRQCPL KVSYGIGDEE HDQEGRVIVA EFDSFVLVTA YVPNAGRGLV
       190        200        210        220        230        240
RLEYRQRWDE AFRKFLKGLA SRKPLVLCGD LNVAHEEIDL RNPKGNKKNA GFTPQERQGF
       250        260        270        280        290        300
GELLQAVPLA DSFRHLYPNT PYAYTFWTYM MNARSKNVGW RLDYFLLSHS LLPALCDSKI
       310
RSKALGSDHC PITLYLAL

Isoforms

Sequence View

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Directness:

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Evidence Codes:

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Protease Assignment Confidence:

Evidence Names:

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Protein Neighborhood

Domains & Features

10 N-termini - 14 C-termini - 4 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P27695-2-unknown	PKRGKK...	2		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P27695-6-unknown	KKGAVA...	6		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10051
P27695-7-	KGAVAE...	7			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P27695-8-unknown	GAVAED...	8		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10052
P27695-9-	AVAEDG...	9			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P27695-9-	AVAEDG...	9			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P27695-14-unknown	GDELRT...	14		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11390
P27695-32-Acetylation	KNDKEA...	32	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P27695-32-	KNDKEA...	32			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P27695-32-unknown	KNDKEA...	32		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10053
P27695-120-	SAPSDK...	120			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P27695-120-	SAPSDK...	120			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...MPKRGK	5	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10051
...KRGKKG	7	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10052
...AVAEDG	13	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11390
...KTAAKK	31	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10053
...SPSGKP	58	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000451491
...KLPAEL	107	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000452304
...VSYGIG	146	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000452137
...AYVPNA	174	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000451170
...AGRGLV	179	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000452418
...RQRWDE	189	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000451060
...DLRNPK	223	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000416414
...NTPYAY	263	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000451327
...WTYMMN	271	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000452460
...LYLAL	318	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAA_HUMAN	5	KRGK.\|.KKGA	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:The substrate specificity profi... (S01.135)	20536382,
GRAA_HUMAN	7	GKKG.\|.GAVA	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:The substrate specificity profi... (S01.135)	20536382,
GRAB_HUMAN	13	AEDG.\|.GDEL	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,
GRAA_HUMAN	31	AAKK.\|.KNDK	inferred from experiment	unknown	MEROPS	Lieberman J	Fan Z et al.:Cleaving the oxidative repair p... (S01.135)	12524539,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)