P29966: Myristoylated alanine-rich C-kinase substrate

General Information

Protein names	- Myristoylated alanine-rich C-kinase substrate - MARCKS - Protein kinase C substrate, 80 kDa protein, light chain - 80K-L protein - PKCSL
Gene names	MARCKS
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P29966

15 N-termini

7 C-termini

6 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MGAQFSKTAA KGEAAAERPG EAAVASSPSK ANGQENGHVK VNGDASPAAA ESGAKEELQA
        70         80         90        100        110        120
NGSAPAADKE EPAAAGSGAA SPSAAEKGEP AAAAAPEAGA SPVEKEAPAE GEAAEPGSPT
       130        140        150        160        170        180
AAEGEAASAA SSTSSPKAED GATPSPSNET PKKKKKRFSF KKSFKLSGFS FKKNKKEAGE
       190        200        210        220        230        240
GGEAEAPAAE GGKDEAAGGA AAAAAEAGAA SGEQAAAPGE EAAAGEEGAA GGDPQEAKPQ
       250        260        270        280        290        300
EAAVAPEKPP ASDETKAAEE PSKVEEKKAE EAGASAAACE APSAAGPGAP PEQEAAPAEE
       310        320        330
PAAAAASSAC AAPSQEAQPE CSPEAPPAEA AE

Isoforms

Sequence View

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Evidence Names:

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Protein Neighborhood

Domains & Features

15 N-termini - 7 C-termini - 6 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P29966-2-unknown	GAQFSK...	2	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P29966-2-unknown	GAQFSK...	2	inferred from similarity	unknown	UniProtKB		inferred from uniprot (by similarity)
P29966-16-	AERPGE...	16		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P29966-43-	GDASPA...	43		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P29966-45-	ASPAAA...	45		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P29966-62-	GSAPAA...	62		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P29966-62-	GSAPAA...	62		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P29966-63-	SAPAAD...	63		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P29966-93-	AAAPEA...	93		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P29966-94-	AAPEAG...	94		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P29966-125-unknown	EAASAA...	125	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19016
P29966-166-unknown	LSGFSF...	166	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3756
P29966-169-unknown	FSFKKN...	169	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3757
P29966-242-unknown	AAVAPE...	242	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7405
P29966-243-unknown	AVAPEK...	243	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7406
P29966-243-	AVAPEK...	243		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P29966-244-unknown	VAPEKP...	244	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7407

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...TAAEGE	124	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19016
...KKSFKL	165	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3756
...FKLSGF	168	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3757
...AKPQEA	241	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7405
...KPQEAA	242	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7406
...PQEAAV	243	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7407
...AEAAE	332	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
MEP1B_HUMAN	124	AEGE.\|.EAAS	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATB_HUMAN	165	SFKL.\|.LSGF	inferred from experiment	unknown	MEROPS	Blackshear PJ	Spizz G et al.:Identification and characteriza... (C01.060)	9295331,
CATB_HUMAN	168	LSGF.\|.FSFK	inferred from experiment	unknown	MEROPS	Blackshear PJ	Spizz G et al.:Identification and characteriza... (C01.060)	9295331,
CATS_HUMAN	241	PQEA.\|.AAVA	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	242	QEAA.\|.AVAP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	243	EAAV.\|.VAPE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)