P48506: Glutamate--cysteine ligase catalytic subunit

General Information

Protein names	- Glutamate--cysteine ligase catalytic subunit - 6.3.2.2 - GCS heavy chain - Gamma-ECS - Gamma-glutamylcysteine synthetase
Gene names	GCLC
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P48506

13 N-termini

7 C-termini

2 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MGLLSQGSPL SWEETKRHAD HVRRHGILQF LHIYHAVKDR HKDVLKWGDE VEYMLVSFDH
        70         80         90        100        110        120
ENKKVRLVLS GEKVLETLQE KGERTNPNHP TLWRPEYGSY MIEGTPGQPY GGTMSEFNTV
       130        140        150        160        170        180
EANMRKRRKE ATSILEENQA LCTITSFPRL GCPGFTLPEV KPNPVEGGAS KSLFFPDEAI
       190        200        210        220        230        240
NKHPRFSTLT RNIRHRRGEK VVINVPIFKD KNTPSPFIET FTEDDEASRA SKPDHIYMDA
       250        260        270        280        290        300
MGFGMGNCCL QVTFQACSIS EARYLYDQLA TICPIVMALS AASPFYRGYV SDIDCRWGVI
       310        320        330        340        350        360
SASVDDRTRE ERGLEPLKNN NYRISKSRYD SIDSYLSKCG EKYNDIDLTI DKEIYEQLLQ
       370        380        390        400        410        420
EGIDHLLAQH VAHLFIRDPL TLFEEKIHLD DANESDHFEN IQSTNWQTMR FKPPPPNSDI
       430        440        450        460        470        480
GWRVEFRPME VQLTDFENSA YVVFVVLLTR VILSYKLDFL IPLSKVDENM KVAQKRDAVL
       490        500        510        520        530        540
QGMFYFRKDI CKGGNAVVDG CGKAQNSTEL AAEEYTLMSI DTIINGKEGV FPGLIPILNS
       550        560        570        580        590        600
YLENMEVDVD TRCSILNYLK LIKKRASGEL MTVARWMREF IANHPDYKQD SVITDEMNYS
       610        620        630
LILKCNQIAN ELCECPELLG SAFRKVKYSG SKTDSSN

Isoforms

Sequence View

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Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

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Protease Assignment Confidence:

Evidence Names:

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Lab:

Protein Neighborhood

Domains & Features

13 N-termini - 7 C-termini - 2 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P48506-1-Acetylation	MGLLSQ...	1	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	THP_untreated	23264352
P48506-2-	GLLSQG...	2			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P48506-2-	GLLSQG...	2			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P48506-2-unknown	GLLSQG...	2			unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P48506-2-unknown	GLLSQG...	2			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P48506-2-unknown	GLLSQG...	2			COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P48506-2-acetylation	GLLSQG...	2	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P48506-3-acetylation	LLSQGS...	3	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P48506-5-	SQGSPL...	5			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P48506-54-unknown	MLVSFD...	54		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000423615
P48506-105-	TPGQPY...	105			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P48506-115-	SEFNTV...	115			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P48506-157-unknown	LPEVKP...	157		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5893
P48506-213-	TPSPFI...	213			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P48506-220-	TFTEDD...	220			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P48506-500-unknown	GCGKAQ...	500		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1858

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...CPGFTL	156	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5893
...INVPIF	207	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000423615
...ETFTED	223	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000424211
...CCLQVT	252	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000421908
...NAVVDG	499	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1858
...TDSSN	637	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...TDSSN	637		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...TDSSN	637		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...TDSSN	637		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...TDSSN	637		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CATL1_HUMAN	156	GFTL.\|.LPEV	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CASP3_HUMAN	499	VVDG.\|.GCGK	inferred from experiment	unknown	MEROPS	Kavanagh TJ	Franklin CC et al.:Caspase-3-Dependent Cleavage of... (C14.003)	12000740,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)