O08529: Calpain-2 catalytic subunit
| Protein names | - Calpain-2 catalytic subunit - 3.4.22.53 - 80 kDa M-calpain subunit - CALP80 - Calcium-activated neutral proteinase 2 - CANP 2 - Calpain M-type - Calpain-2 large subunit - Millimolar-calpain - M-calpain |
|---|---|
| Gene names | Capn2 |
| Organism | Mus musculus |
| Protease Family | C02.002 |
| Protease ID | C02.002 |
| Chromosome location | |
| UniProt ID | O08529 |
6
N-termini
2
C-termini
1
Cleavages
23
Substrates
Sequence
10 20 30 40 50 60
MAGIAIKLAK DREAAEGLGS HERAIKYLNQ DYETLRNECL EAGALFQDPS FPALPSSLGY
70 80 90 100 110 120
KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG ALGDCWLLAA IASLTLNEEI
130 140 150 160 170 180
LARVVPPDQS FQENYAGIFH FQFWQYGEWV EVVVDDRLPT KDGELLFVHS AEGSEFWSAL
190 200 210 220 230 240
LEKAYAKING CYEALSGGAT TEGFEDFTGG IAEWYELRKP PPNLFKIIQK ALEKGSLLGC
250 260 270 280 290 300
SIDITSAADS EAVTYQKLVK GHAYSVTGAE EVESSGSLQK LIRIRNPWGQ VEWTGKWNDN
310 320 330 340 350 360
CPSWNTVDPE VRANLTERQE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTC DSYKKWKLTK
370 380 390 400 410 420
MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED GERGCTFLVG LIQKHRRRQR
430 440 450 460 470 480
KMGEDMHTIG FGIYEVPEEL TGQTNIHLGK NFFLTTRARE RSDTFINLRE VLNRFKLPPG
490 500 510 520 530 540
EYVLVPSTFE PHKDGDFCIR VFSEKKADYQ AVDDEIEANI EEIDANEEDI DDGFRRLFVQ
550 560 570 580 590 600
LAGEDAEISA FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDEDGSG KLGLKEFYIL
610 620 630 640 650 660
WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKLPC QLHQVIVARF ADDELIIDFD
670 680 690 700
NFVRCLVRLE TLFKIFKQLD PENTGTIQLN LASWLSFSVL
Isoforms
Sequence View
Protein Neighborhood
Domains & Features
6 N-termini - 2 C-termini - 1 Cleavages - 23 Substrates
N-termini
| Name | Sequence | Position | Modification | Evidence type | Method | Source (database) | Source (Lab) | Evidence name | Publications (PMID) |
|---|---|---|---|---|---|---|---|---|---|
| O08529-2-unknown | MAGIAI... | 1 | inferred from similarity | unknown | UniProtKB | inferred from uniprot (by similarity) | |||
| O08529-2-unknown | MAGIAI... | 1 | inferred from electronic annotation | electronic annotation | UniProtKB | inferred from uniprot | |||
| O08529-2-Acetylation | AGIAIK... | 2 | acetylation- | N-TAILS | Overall CM. | Kleifeldt O. et al.: Isotopic labeling of terminal amines in complex samples identifies... | 20208520 | ||
| O08529-2-Acetylation | AGIAIK... | 2 | acetylation- | unknown | Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from... | 20208520 | |||
| O08529-2-Acetylation | AGIAIK... | 2 | acetylation- | inferred from electronic annotation | electronic annotation | UniProtKB | inferred from uniprot | ||
| O08529-2-Acetylation | AGIAIK... | 2 | acetylation- | inferred from similarity | unknown | UniProtKB | inferred from uniprot (by similarity) | ||
| O08529-20-unknown | SHERAI... | 20 | inferred from electronic annotation | electronic annotation | UniProtKB | inferred from uniprot | |||
| O08529-30-unknown | QDYETL... | 30 | inferred from electronic annotation | unknown | TISdb | inferred from TISdb | |||
| O08529-77-unknown | RPTEIC... | 77 | inferred from cleavage | unknown | TopFIND | Inferred from cleavage TC33129 | |||
| O08529-261-unmodified | GHAYSV... | 261 | N-TAILS | Overall CM. | Prudova A.: Multiplex N-terminome analysis of MMP-2 and MMP-9... | 20305284 |
C-termini
| Name | Sequence | Position | Evidence type | Method | Source (database) | Source (Lab) | Evidence name | Publications (PMIDs) |
|---|---|---|---|---|---|---|---|---|
| ...GIEWKR | 76 | inferred from cleavage | unknown | TopFIND | Inferred from cleavage TC33129 | |||
| ...SFSVL | 700 | inferred from electronic annotation | electronic annotation | UniProtKB | inferred from uniprot |
Cleavages
| Protease | Position | Sequence | Evidence type | Method | Source (database) | Source (Lab) | Evidence name | Publications (PMIDs) |
|---|---|---|---|---|---|---|---|---|
| MMP2_MOUSE | 76 | EWKR.|.RPTE | inferred from experiment | unknown | MEROPS | Overall CM | Prudova A et al.:Multiplex N-terminome analysis ... (M10.004) | 20305284, |
Substrates
| Substrate | Position | Sequence | Evidence type | Method | Source (database) | Source (Lab) | Evidence name | Publications (PMIDs) |
|---|---|---|---|---|---|---|---|---|
| VIME_MOUSE | 19 | GSGT.|.TSSR | inferred from experiment | unknown | MEROPS | Weber K | Fischer S et al.:Protein-chemical identification... (C02.002) | 3028449, |
| VIME_MOUSE | 21 | GTSS.|.SRPS | inferred from experiment | unknown | MEROPS | Weber K | Fischer S et al.:Protein-chemical identification... (C02.002) | 3028449, |
| KCC4_MOUSE | 23 | TENL.|.LVPD | inferred from experiment | unknown | MEROPS | Wang KK | McGinnis KM et al.:Calcium/calmodulin-dependent pr... (C02.001) | 9685336, |
| VIME_MOUSE | 33 | VTTS.|.STRT | inferred from experiment | unknown | MEROPS | Weber K | Fischer S et al.:Protein-chemical identification... (C02.002) | 3028449, |
| VIME_MOUSE | 38 | RTYS.|.SLGS | inferred from experiment | unknown | MEROPS | Weber K | Fischer S et al.:Protein-chemical identification... (C02.002) | 3028449, |
| VIME_MOUSE | 41 | SLGS.|.SALR | inferred from experiment | unknown | MEROPS | Weber K | Fischer S et al.:Protein-chemical identification... (C02.002) | 3028449, |
| FAK1-Isoform9_MOUSE | 53 | QVSG.|.GYPG | inferred from isoform by sequence similarity | unknown | TopFIND | inferred from TC28327 | ||
| VIME_MOUSE | 53 | SLYS.|.SSSP | inferred from experiment | unknown | MEROPS | Weber K | Fischer S et al.:Protein-chemical identification... (C02.002) | 3028449, |
| B2CL1_MOUSE | 60 | HLAD.|.DSPA | inferred from experiment | unknown | MEROPS | Yuan J | Nakagawa T et al.:Cross-talk between two cysteine... (C02.002) | 10953012, |
| B2CL1-Isoform4_MOUSE | 60 | HLAD.|.DSPA | inferred from isoform by sequence similarity | unknown | TopFIND | inferred from TC28334 | ||
| B2CL1-Isoform3_MOUSE | 60 | HLAD.|.DSPA | inferred from isoform by sequence similarity | unknown | TopFIND | inferred from TC28334 | ||
| B2CL1-Isoform2_MOUSE | 60 | HLAD.|.DSPA | inferred from isoform by sequence similarity | unknown | TopFIND | inferred from TC28334 | ||
| VIME_MOUSE | 64 | VTRS.|.SSAV | inferred from experiment | unknown | MEROPS | Weber K | Fischer S et al.:Protein-chemical identification... (C02.002) | 3028449, |
| VIME_MOUSE | 71 | RLRS.|.SSVP | inferred from experiment | unknown | MEROPS | Weber K | Fischer S et al.:Protein-chemical identification... (C02.002) | 3028449, |
| CASPC_MOUSE | 132 | MLTA.|.APHG | inferred from experiment | unknown | MEROPS | Yuan J | Nakagawa T et al.:Cross-talk between two cysteine... (C02.002) | 10953012, |
| CASPC_MOUSE | 158 | KIKT.|.TERA | inferred from experiment | unknown | MEROPS | Yuan J | Nakagawa T et al.:Cross-talk between two cysteine... (C02.002) | 10953012, |
| KCC4_MOUSE | 199 | VCGT.|.TPGY | inferred from experiment | unknown | MEROPS | Wang KK | McGinnis KM et al.:Calcium/calmodulin-dependent pr... (C02.001) | 9685336, |
| FAK1-Isoform6_MOUSE | 745 | QVSG.|.GYPG | inferred from isoform by sequence similarity | unknown | TopFIND | inferred from TC28327 | ||
| FAK1-Isoform4_MOUSE | 745 | QVSG.|.GYPG | inferred from isoform by sequence similarity | unknown | TopFIND | inferred from TC28327 | ||
| FAK1-Isoform3_MOUSE | 745 | QVSG.|.GYPG | inferred from isoform by sequence similarity | unknown | TopFIND | inferred from TC28327 | ||
| FAK1-Isoform2_MOUSE | 776 | QVSG.|.GYPG | inferred from isoform by sequence similarity | unknown | TopFIND | inferred from TC28327 | ||
| FAK1_MOUSE | 783 | MSMW.|.WQPS | inferred from experiment | unknown | MEROPS | Huttenlocher A | Chan KT et al.:Regulation of adhesion dynamics... (C02.002) | 20150423, |
| FAK1-Isoform5_MOUSE | 786 | QVSG.|.GYPG | inferred from isoform by sequence similarity | unknown | TopFIND | inferred from TC28327 |