O60841: Eukaryotic translation initiation factor 5B

General Information

Protein names	- Eukaryotic translation initiation factor 5B - eIF-5B - 3.6.5.3 - Translation initiation factor IF-2
Gene names	EIF5B
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	O60841

11 N-termini

8 C-termini

8 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MGKKQKNKSE DSTKDDIDLD ALAAEIEGAG AAKEQEPQKS KGKKKKEKKK QDFDEDDILK
        70         80         90        100        110        120
ELEELSLEAQ GIKADRETVA VKPTENNEEE FTSKDKKKKG QKGKKQSFDD NDSEELEDKD
       130        140        150        160        170        180
SKSKKTAKPK VEMYSGSDDD DDFNKLPKKA KGKAQKSNKK WDGSEEDEDN SKKIKERSRI
       190        200        210        220        230        240
NSSGESGDES DEFLQSRKGQ KKNQKNKPGP NIESGNEDDD ASFKIKTVAQ KKAEKKERER
       250        260        270        280        290        300
KKRDEEKAKL RKLKEKEELE TGKKDQSKQK ESQRKFEEET VKSKVTVDTG VIPASEEKAE
       310        320        330        340        350        360
TPTAAEDDNE GDKKKKDKKK KKGEKEEKEK EKKKGPSKAT VKAMQEALAK LKEEEERQKR
       370        380        390        400        410        420
EEEERIKRLE ELEAKRKEEE RLEQEKRERK KQKEKERKER LKKEGKLLTK SQREARARAE
       430        440        450        460        470        480
ATLKLLQAQG VEVPSKDSLP KKRPIYEDKK RKKIPQQLES KEVSESMELC AAVEVMEQGV
       490        500        510        520        530        540
PEKEETPPPV EPEEEEDTED AGLDDWEAMA SDEETEKVEG NKVHIEVKEN PEEEEEEEEE
       550        560        570        580        590        600
EEEDEESEEE EEEEGESEGS EGDEEDEKVS DEKDSGKTLD KKPSKEMSSD SEYDSDDDRT
       610        620        630        640        650        660
KEERAYDKAK RRIEKRRLEH SKNVNTEKLR APIICVLGHV DTGKTKILDK LRHTHVQDGE
       670        680        690        700        710        720
AGGITQQIGA TNVPLEAINE QTKMIKNFDR ENVRIPGMLI IDTPGHESFS NLRNRGSSLC
       730        740        750        760        770        780
DIAILVVDIM HGLEPQTIES INLLKSKKCP FIVALNKIDR LYDWKKSPDS DVAATLKKQK
       790        800        810        820        830        840
KNTKDEFEER AKAIIVEFAQ QGLNAALFYE NKDPRTFVSL VPTSAHTGDG MGSLIYLLVE
       850        860        870        880        890        900
LTQTMLSKRL AHCEELRAQV MEVKALPGMG TTIDVILING RLKEGDTIIV PGVEGPIVTQ
       910        920        930        940        950        960
IRGLLLPPPM KELRVKNQYE KHKEVEAAQG VKILGKDLEK TLAGLPLLVA YKEDEIPVLK
       970        980        990       1000       1010       1020
DELIHELKQT LNAIKLEEKG VYVQASTLGS LEALLEFLKT SEVPYAGINI GPVHKKDVMK
      1030       1040       1050       1060       1070       1080
ASVMLEHDPQ YAVILAFDVR IERDAQEMAD SLGVRIFSAE IIYHLFDAFT KYRQDYKKQK
      1090       1100       1110       1120       1130       1140
QEEFKHIAVF PCKIKILPQY IFNSRDPIVM GVTVEAGQVK QGTPMCVPSK NFVDIGIVTS
      1150       1160       1170       1180       1190       1200
IEINHKQVDV AKKGQEVCVK IEPIPGESPK MFGRHFEATD ILVSKISRQS IDALKDWFRD
      1210       1220
EMQKSDWQLI VELKKVFEII

Isoforms

Sequence View

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Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

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Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

11 N-termini - 8 C-termini - 8 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
O60841-1-unknown	MGKKQK...	1	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
O60841-21-unknown	ALAAEI...	21		unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
O60841-21-unknown	ALAAEI...	21		unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
O60841-21-	ALAAEI...	21		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
O60841-21-	ALAAEI...	21		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
O60841-21-	ALAAEI...	21		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O60841-21-	ALAAEI...	21		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
O60841-21-	ALAAEI...	21		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
O60841-63-unknown	EELSLE...	63	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18294
O60841-81-unknown	VKPTEN...	81	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5216
O60841-81-unknown	VKPTEN...	81	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7056
O60841-135-	SGSDDD...	135		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
O60841-260-unknown	ETGKKD...	260	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13776
O60841-349-unknown	AKLKEE...	349	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13777
O60841-402-unknown	KKEGKL...	402	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13778
O60841-427-unknown	QAQGVE...	427	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13779
O60841-430-unknown	GVEVPS...	430	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13780

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...ILKELE	62	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18294
...RETVAV	80	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5216
...RETVAV	80	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7056
...EKEELE	259	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC13776
...MQEALA	348	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC13777
...RKERLK	401	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC13778
...TLKLLQ	426	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC13779
...LLQAQG	429	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC13780
...VFEII	1220	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
MEP1B_HUMAN	62	KELE.\|.EELS	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATL1_HUMAN	80	TVAV.\|.VKPT	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	80	TVAV.\|.VKPT	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
GRAM_HUMAN	259	EELE.\|.ETGK	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,
GRAM_HUMAN	348	EALA.\|.AKLK	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,
GRAM_HUMAN	401	ERLK.\|.KKEG	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,
GRAM_HUMAN	426	KLLQ.\|.QAQG	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,
GRAM_HUMAN	429	QAQG.\|.GVEV	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)