P06454: Prothymosin alpha

General Information

Protein names	- Prothymosin alpha - Prothymosin alpha, N-terminally processed - Thymosin alpha-1
Gene names	PTMA
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P06454

34 N-termini

22 C-termini

23 Cleavages

0 Substrates

Sequence

10 20 30 40 50 60
MSDAAVDTSS EITTKDLKEK KEVVEEAENG RDAPANGNAE NEENGEQEAD NEVDEEEEEG
70 80 90 100 110
GEEEEEEEEG DGEEEDGDED EEAESATGKR AAEDDEDDDV DTKKQKTDED D

Isoforms

- Isoform 2 of Prothymosin alpha

Sequence View

10 20 30 40 50 60
MSDAAVDTSS EITTKDLKEK KEVVEEAENG RDAPANGNAE NEENGEQEAD NEVDEEEEEG
70 80 90 100 110
GEEEEEEEEG DGEEEDGDED EEAESATGKR AAEDDEDDDV DTKKQKTDED D

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Physiological Relevance:

Evidence Codes:

Methodology:

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Protease Assignment Confidence:

Evidence Names:

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Lab:

Protein Neighborhood

Domains & Features

34 N-termini - 22 C-termini - 23 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P06454-1-Acetylation	MSDAAV...	1	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P06454-1-Acetylation	MSDAAV...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt100346
P06454-1-unknown	MSDAAV...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt86487
P06454-2-Acetylation	SDAAVD...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P06454-2-Acetylation	SDAAVD...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P06454-2-Acetylation	SDAAVD...	2	acetylation-		other		CNRS/ISV	Large_scale_NTA_Human
P06454-2-Acetylation	SDAAVD...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P06454-2-Acetylation	SDAAVD...	2	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt110611
P06454-2-	SDAAVD...	2			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P06454-2-unknown	SDAAVD...	2		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt172183
P06454-4-unknown	AAVDTS...	4		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17150
P06454-4-unknown	AAVDTS...	4			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P06454-4-unknown	AAVDTS...	4		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt161952
P06454-4-	AAVDTS...	4			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P06454-4-	AAVDTS...	4			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P06454-4-	AAVDTS...	4			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P06454-4-	AAVDTS...	4			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P06454-4-	AAVDTS...	4			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P06454-4-	AAVDTS...	4			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P06454-4-	AAVDTS...	4			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P06454-4-	AAVDTS...	4			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P06454-5-unknown	AVDTSS...	5			unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P06454-5-unknown	AVDTSS...	5		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt160082
P06454-5-	AVDTSS...	5			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P06454-5-	AVDTSS...	5			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P06454-5-	AVDTSS...	5			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P06454-8-	TSSEIT...	8			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P06454-8-	TSSEIT...	8			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P06454-8-unknown	TSSEIT...	8		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1575
P06454-8-unknown	TSSEIT...	8		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11055
P06454-8-unknown	TSSEIT...	8			unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P06454-8-unknown	TSSEIT...	8		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt160083
P06454-10-unknown	SEITTK...	10		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11056
P06454-10-unknown	SEITTK...	10		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt194861
P06454-14-unknown	TKDLKE...	14		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11057
P06454-14-unknown	TKDLKE...	14		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt136463
P06454-14-unknown	TKDLKE...	14		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P06454-14-unknown	TKDLKE...	14		inferred from similarity	unknown	UniProtKB		inferred from uniprot (by similarity)
P06454-16-unknown	DLKEKK...	16		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC9936
P06454-16-unknown	DLKEKK...	16		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt136604
P06454-16-unknown	DLKEKK...	16		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P06454-16-unknown	DLKEKK...	16		inferred from similarity	unknown	UniProtKB		inferred from uniprot (by similarity)
P06454-17-unknown	LKEKKE...	17		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11059
P06454-17-unknown	LKEKKE...	17		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt136620
P06454-19-	EKKEVV...	19			Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P06454-19-	EKKEVV...	19			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P06454-19-unknown	EKKEVV...	19		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11061
P06454-19-unknown	EKKEVV...	19		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt136772
P06454-21-unknown	KEVVEE...	21		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11063
P06454-21-	KEVVEE...	21			Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P06454-21-	KEVVEE...	21			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P06454-21-	KEVVEE...	21			Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
P06454-21-	KEVVEE...	21			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P06454-22-unknown	EVVEEA...	22		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11065
P06454-30-unknown	GRDAPA...	30		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC16750
P06454-37-unknown	GNAENE...	37		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC16752
P06454-60-unknown	GGEEEE...	60		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11067
P06454-60-unknown	GGEEEE...	60		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt139805
P06454-63-unknown	EEEEEE...	63		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11069
P06454-63-unknown	EEEEEE...	63		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt140083
P06454-66-unknown	EEEEGD...	66		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11071
P06454-66-unknown	EEEEGD...	66		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt140281
P06454-69-unknown	EGDGEE...	69		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11073
P06454-69-unknown	EGDGEE...	69		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt140491
P06454-72-unknown	GEEEDG...	72		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11075
P06454-72-unknown	GEEEDG...	72		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt140706
P06454-77-unknown	GDEDEE...	77		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11077
P06454-77-unknown	GDEDEE...	77		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt141029
P06454-78-unknown	DEDEEA...	78		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11079
P06454-78-unknown	DEDEEA...	78		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt141101
P06454-91-unknown	AAEDDE...	91		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt164351
P06454-91-	AAEDDE...	91			Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P06454-91-	AAEDDE...	91			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P06454-91-	AAEDDE...	91			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P06454-98-unknown	DDVDTK...	98		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1576
P06454-98-unknown	DDVDTK...	98		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt142237
P06454-99-unknown	DVDTKK...	99		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1578
P06454-99-unknown	DVDTKK...	99		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt142274
P06454-102-unknown	TKKQKT...	102		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1580
P06454-102-unknown	TKKQKT...	102		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2796
P06454-102-unknown	TKKQKT...	102		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt142439

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...DAAVDT	7	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1575
...DAAVDT	7	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11055
...AVDTSS	9	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11056
...SSEITT	13	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11057
...EITTKD	15	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC9936
...ITTKDL	16	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11059
...TKDLKE	18	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11061
...DLKEKK	20	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11063
...LKEKKE	21	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11065
...LKEKKE	21	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt119808
...EEAENG	29	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC16750
...EEAENG	29	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt120533
...DAPANG	36	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC16752
...DAPANG	36	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt153782
...EEEEEG	59	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11067
...EEGGEE	62	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11069
...EEGGEE	62	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt123190
...GEEEEE	65	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11071
...GEEEEE	65	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt123403
...EEEEEE	68	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11073
...EEEEEE	68	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt123618
...EEEGDG	71	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11075
...EEEGDG	71	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt123841
...GEEEDG	76	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11077
...GEEEDG	76	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt124179
...EEEDGD	77	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11079
...EEEDGD	77	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt124255
...EDDEDD	97	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1576
...EDDEDD	97	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt125425
...DDEDDD	98	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1578
...DDEDDD	98	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt125464
...DDDVDT	101	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1580
...DDDVDT	101	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2796
...DDDVDT	101	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt125633
...TDEDD	111		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...TDEDD	111		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...TDEDD	111	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...TDEDD	111	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt82105
...TDEDD	111		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...TDEDD	111		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
MEP1A_HUMAN	3	MSDA.\|.AAVD	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CASP3_HUMAN	7	AVDT.\|.TSSE	inferred from experiment	unknown	MEROPS		Merops CASP3_HUMAN -> PTMA_HUMAN @7
GRAB_HUMAN	7	AVDT.\|.TSSE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	9	DTSS.\|.SEIT	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,
GRAB_HUMAN	13	EITT.\|.TKDL	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAA_HUMAN	15	TTKD.\|.DLKE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:The substrate specificity profi... (S01.135)	20536382,
GRAB_HUMAN	16	TKDL.\|.LKEK	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	18	DLKE.\|.EKKE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	20	KEKK.\|.KEVV	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	21	EKKE.\|.EVVE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
LGMN_HUMAN	29	AENG.\|.GRDA	inferred from experiment	unknown	MEROPS	Freire M	Sarandeses CS et al.:Prothymosin alpha is processed ... (C13.004)	12554742,
LGMN_HUMAN	36	PANG.\|.GNAE	inferred from experiment	unknown	MEROPS	Freire M	Sarandeses CS et al.:Prothymosin alpha is processed ... (C13.004)	12554742,
GRAB_HUMAN	59	EEEG.\|.GGEE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	62	GGEE.\|.EEEE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	65	EEEE.\|.EEEE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	68	EEEE.\|.EGDG	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	71	EGDG.\|.GEEE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	76	EEDG.\|.GDED	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAB_HUMAN	77	EDGD.\|.DEDE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
CASP3_HUMAN	97	DEDD.\|.DDVD	inferred from experiment	unknown	MEROPS	Berger SL	Enkemann SA et al.:Functional discontinuities in p... (C14.003)	10623890,
CASP3_HUMAN	98	EDDD.\|.DVDT	inferred from experiment	unknown	MEROPS	Berger SL	Enkemann SA et al.:Functional discontinuities in p... (C14.003)	10623890,
CASP3_HUMAN	101	DVDT.\|.TKKQ	inferred from experiment	unknown	MEROPS	Berger SL	Enkemann SA et al.:Functional discontinuities in p... (C14.003)	10623890,
CASP7_HUMAN	101	DVDT.\|.TKKQ	inferred from experiment	unknown	MEROPS	Vartapetian AB	Evstafieva AG et al.:Prothymosin alpha fragmentation... (C14.004)	10675528,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)