P26639: Threonine--tRNA ligase, cytoplasmic

General Information

Protein names	- Threonine--tRNA ligase, cytoplasmic - 6.1.1.3 {ECO:0000269\|PubMed:25824639} - Threonyl-tRNA synthetase - ThrRS
Gene names	TARS
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P26639

15 N-termini

5 C-termini

3 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MFEEKASSPS GKMGGEEKPI GAGEEKQKEG GKKKNKEGSG DGGRAELNPW PEYIYTRLEM
        70         80         90        100        110        120
YNILKAEHDS ILAEKAEKDS KPIKVTLPDG KQVDAESWKT TPYQIACGIS QGLADNTVIA
       130        140        150        160        170        180
KVNNVVWDLD RPLEEDCTLE LLKFEDEEAQ AVYWHSSAHI MGEAMERVYG GCLCYGPPIE
       190        200        210        220        230        240
NGFYYDMYLE EGGVSSNDFS SLEALCKKII KEKQAFERLE VKKETLLAMF KYNKFKCRIL
       250        260        270        280        290        300
NEKVNTPTTT VYRCGPLIDL CRGPHVRHTG KIKALKIHKN SSTYWEGKAD METLQRIYGI
       310        320        330        340        350        360
SFPDPKMLKE WEKFQEEAKN RDHRKIGRDQ ELYFFHELSP GSCFFLPKGA YIYNALIEFI
       370        380        390        400        410        420
RSEYRKRGFQ EVVTPNIFNS RLWMTSGHWQ HYSENMFSFE VEKELFALKP MNCPGHCLMF
       430        440        450        460        470        480
DHRPRSWREL PLRLADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCAME QIEDEIKGCL
       490        500        510        520        530        540
DFLRTVYSVF GFSFKLNLST RPEKFLGDIE VWDQAEKQLE NSLNEFGEKW ELNSGDGAFY
       550        560        570        580        590        600
GPKIDIQIKD AIGRYHQCAT IQLDFQLPIR FNLTYVSHDG DDKKRPVIVH RAILGSVERM
       610        620        630        640        650        660
IAILTENYGG KWPFWLSPRQ VMVVPVGPTC DEYAQKVRQQ FHDAKFMADI DLDPGCTLNK
       670        680        690        700        710        720
KIRNAQLAQY NFILVVGEKE KISGTVNIRT RDNKVHGERT ISETIERLQQ LKEFRSKQAE

EEF

Isoforms

- Isoform 2 of Threonine--tRNA ligase, cytoplasmic

Sequence View

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Protein Neighborhood

Domains & Features

15 N-termini - 5 C-termini - 3 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P26639-1-	MFEEKA...	1			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P26639-1-acetylation	MFEEKA...	1	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P26639-1-acetylation	MFEEKA...	1	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt182576
P26639-1-unknown	MFEEKA...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P26639-1-unknown	MFEEKA...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt91285
P26639-1-unknown	MFEEKA...	1			COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P26639-6-	ASSPSG...	6			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P26639-6-unknown	ASSPSG...	6		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt169619
P26639-13-	MGGEEK...	13			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P26639-13-unknown	MGGEEK...	13		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt169621
P26639-14-unknown	GGEEKP...	14			unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P26639-14-unknown	GGEEKP...	14			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P26639-14-unknown	GGEEKP...	14		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt160337
P26639-14-	GGEEKP...	14			Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P26639-14-	GGEEKP...	14			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P26639-14-	GGEEKP...	14			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P26639-14-	GGEEKP...	14			Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
P26639-14-	GGEEKP...	14			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P26639-14-	GGEEKP...	14			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P26639-14-	GGEEKP...	14			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P26639-14-	GGEEKP...	14			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P26639-14-	GGEEKP...	14			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P26639-90-unknown	GKQVDA...	90		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt177900
P26639-90-	GKQVDA...	90			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P26639-108-unknown	GISQGL...	108		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3740
P26639-111-unknown	QGLADN...	111		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5739
P26639-111-unknown	QGLADN...	111		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt197113
P26639-172-unknown	CLCYGP...	172		inferred from electronic annotation	unknown	TISdb		inferred from TISdb
P26639-172-unknown	CLCYGP...	172		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt197833
P26639-534-unknown	SGDGAF...	534		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7390
P26639-534-unknown	SGDGAF...	534		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt154193

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...YQIACG	107	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3740
...YQIACG	107	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt154449
...ACGISQ	110	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5739
...ACGISQ	110	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt154481
...KWELNS	533	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7390
...KWELNS	533	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt137613
...AEEEF	723		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...AEEEF	723	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...AEEEF	723	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt86903
...AEEEF	723		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...AEEEF	723		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CATB_HUMAN	107	IACG.\|.GISQ	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	110	GISQ.\|.QGLA	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	533	ELNS.\|.SGDG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)