P60842: Eukaryotic initiation factor 4A-I

General Information

Protein names	- Eukaryotic initiation factor 4A-I - eIF-4A-I - eIF4A-I - 3.6.4.13 - ATP-dependent RNA helicase eIF4A-1
Gene names	EIF4A1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P60842

23 N-termini

4 C-termini

3 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY GFEKPSAIQQ
        70         80         90        100        110        120
RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA TQALVLAPTR ELAQQIQKVV
       130        140        150        160        170        180
MALGDYMGAS CHACIGGTNV RAEVQKLQME APHIIVGTPG RVFDMLNRRY LSPKYIKMFV
       190        200        210        220        230        240
LDEADEMLSR GFKDQIYDIF QKLNSNTQVV LLSATMPSDV LEVTKKFMRD PIRILVKKEE
       250        260        270        280        290        300
LTLEGIRQFY INVEREEWKL DTLCDLYETL TITQAVIFIN TRRKVDWLTE KMHARDFTVS
       310        320        330        340        350        360
AMHGDMDQKE RDVIMREFRS GSSRVLITTD LLARGIDVQQ VSLVINYDLP TNRENYIHRI
       370        380        390        400
GRGGRFGRKG VAINMVTEED KRTLRDIETF YNTSIEEMPL NVADLI

Isoforms

- Isoform 2 of Eukaryotic initiation factor 4A-I

Sequence View

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

23 N-termini - 4 C-termini - 3 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P60842-1-unknown	MSASQD...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt77541
P60842-2-unknown	MSASQD...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P60842-2-Acetylation	SASQDS...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P60842-2-Acetylation	SASQDS...	2	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt111374
P60842-16-	GMEPEG...	16			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P60842-16-	GMEPEG...	16			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P60842-16-unknown	GMEPEG...	16		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175455
P60842-33-	SFDDMN...	33			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P60842-33-	SFDDMN...	33			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P60842-33-	SFDDMN...	33			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P60842-33-unknown	SFDDMN...	33		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt176501
P60842-46-unknown	GIYAYG...	46		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt177631
P60842-46-	GIYAYG...	46			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P60842-49-unknown	AYGFEK...	49		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt172162
P60842-49-	AYGFEK...	49			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P60842-49-	AYGFEK...	49			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P60842-49-	AYGFEK...	49			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P60842-150-unknown	EAPHII...	150		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19233
P60842-150-unknown	EAPHII...	150		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt144713
P60842-178-	MFVLDE...	178			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P60842-178-	MFVLDE...	178			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P60842-178-	MFVLDE...	178			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P60842-178-	MFVLDE...	178			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P60842-178-	MFVLDE...	178			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P60842-178-	MFVLDE...	178			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P60842-178-unknown	MFVLDE...	178		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt167485
P60842-179-unknown	FVLDEA...	179		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt170494
P60842-179-	FVLDEA...	179			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P60842-213-unknown	SATMPS...	213		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt167487
P60842-213-	SATMPS...	213			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P60842-213-	SATMPS...	213			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P60842-213-	SATMPS...	213			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P60842-301-unknown	AMHGDM...	301		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6072
P60842-301-unknown	AMHGDM...	301		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7609
P60842-301-unknown	AMHGDM...	301		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt149904
P60842-301-	AMHGDM...	301			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P60842-302-unknown	MHGDMD...	302		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt170496
P60842-302-	MHGDMD...	302			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P60842-342-	SLVINY...	342			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...QKLQME	149	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19233
...QKLQME	149	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt127981
...DFTVSA	300	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6072
...DFTVSA	300	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7609
...DFTVSA	300	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt133296
...VADLI	406		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...VADLI	406	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...VADLI	406		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
MEP1B_HUMAN	149	LQME.\|.EAPH	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATL1_HUMAN	300	TVSA.\|.AMHG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	300	TVSA.\|.AMHG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)