Q13283: Ras GTPase-activating protein-binding protein 1

General Information

Protein names	- Ras GTPase-activating protein-binding protein 1 - G3BP-1 - 3.6.4.12 {ECO:0000269\|PubMed:9889278} - 3.6.4.13 {ECO:0000269\|PubMed:9889278} - ATP-dependent DNA helicase VIII - hDH VIII - GAP SH3 domain-binding protein 1
Gene names	G3BP1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	Q13283

22 N-termini

9 C-termini

7 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MVMEKPSPLL VGREFVRQYY TLLNQAPDML HRFYGKNSSY VHGGLDSNGK PADAVYGQKE
        70         80         90        100        110        120
IHRKVMSQNF TNCHTKIRHV DAHATLNDGV VVQVMGLLSN NNQALRRFMQ TFVLAPEGSV
       130        140        150        160        170        180
ANKFYVHNDI FRYQDEVFGG FVTEPQEESE EEVEEPEERQ QTPEVVPDDS GTFYDQAVVS
       190        200        210        220        230        240
NDMEEHLEEP VAEPEPDPEP EPEQEPVSEI QEEKPEPVLE ETAPEDAQKS SSPAPADIAQ
       250        260        270        280        290        300
TVQEDLRTFS WASVTSKNLP PSGAVPVTGI PPHVVKVPAS QPRPESKPES QIPPQRPQRD
       310        320        330        340        350        360
QRVREQRINI PPQRGPRPIR EAGEQGDIEP RRMVRHPDSH QLFIGNLPHE VDKSELKDFF
       370        380        390        400        410        420
QSYGNVVELR INSGGKLPNF GFVVFDDSEP VQKVLSNRPI MFRGEVRLNV EEKKTRAARE
       430        440        450        460
GDRRDNRLRG PGGPRGGLGG GMRGPPRGGM VQKPGFGVGR GLAPRQ

Isoforms

- Isoform 2 of Ras GTPase-activating protein-binding protein 1

Sequence View

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Protein Neighborhood

Domains & Features

22 N-termini - 9 C-termini - 7 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
Q13283-1-	MVMEKP...	1			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
Q13283-1-	MVMEKP...	1			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
Q13283-1-	MVMEKP...	1			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q13283-1-	MVMEKP...	1			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
Q13283-1-acetylation	MVMEKP...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
Q13283-1-acetylation	MVMEKP...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
Q13283-2-unknown	VMEKPS...	2		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
Q13283-2-unknown	VMEKPS...	2			COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
Q13283-2-unknown	VMEKPS...	2			COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	RMPI_untreated	23264352
Q13283-2-	VMEKPS...	2			Subtiligase Based Positive Selection		Wells	THP_untreated	23264352
Q13283-3-acetylation	MEKPSP...	3	acetylation-		other		CNRS/ISV	Large_scale_NTA_Human
Q13283-24-unknown	NQAPDM...	24		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6323
Q13283-25-unknown	QAPDML...	25		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6324
Q13283-49-	GKPADA...	49			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
Q13283-96-	GLLSNN...	96			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
Q13283-119-	SVANKF...	119			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q13283-124-	FYVHND...	124			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q13283-140-	GFVTEP...	140			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q13283-183-unknown	MEEHLE...	183		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000445035
Q13283-186-	HLEEPV...	186			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q13283-186-	HLEEPV...	186			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
Q13283-192-	AEPEPD...	192			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q13283-192-	AEPEPD...	192			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
Q13283-230-	SSSPAP...	230			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q13283-230-	SSSPAP...	230			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q13283-230-	SSSPAP...	230			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
Q13283-231-	SSPAPA...	231			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q13283-264-unknown	AVPVTG...	264			unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
Q13283-264-unknown	AVPVTG...	264			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
Q13283-264-	AVPVTG...	264			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
Q13283-264-	AVPVTG...	264			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
Q13283-264-	AVPVTG...	264			Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
Q13283-264-	AVPVTG...	264			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
Q13283-264-	AVPVTG...	264			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q13283-264-	AVPVTG...	264			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
Q13283-264-	AVPVTG...	264			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
Q13283-269-unknown	GIPPHV...	269		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7768
Q13283-269-unknown	GIPPHV...	269		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6325
Q13283-371-	INSGGK...	371			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
Q13283-382-unknown	FVVFDD...	382		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7769
Q13283-382-unknown	FVVFDD...	382		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3985
Q13283-382-unknown	FVVFDD...	382		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6326

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...YYTLLN	23	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6323
...YTLLNQ	24	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6324
...NNQALR	105	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000428469
...NQALRR	106	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000430738
...VLAPEG	117	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000429920
...QDEVFG	138	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000429250
...AVPVTG	268	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7768
...AVPVTG	268	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6325
...LPNFGF	381	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7769
...LPNFGF	381	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3985
...LPNFGF	381	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6326
...LAPRQ	466	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CATL1_HUMAN	23	TLLN.\|.NQAP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	24	LLNQ.\|.QAPD	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	268	PVTG.\|.GIPP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	268	PVTG.\|.GIPP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	381	NFGF.\|.FVVF	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	381	NFGF.\|.FVVF	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	381	NFGF.\|.FVVF	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)