Q9UHX1: Poly(U)-binding-splicing factor PUF60

General Information

Protein names	- Poly(U)-binding-splicing factor PUF60 - 60 kDa poly(U)-binding-splicing factor - FUSE-binding protein-interacting repressor - FBP-interacting repressor - Ro-binding protein 1 - RoBP1 - Siah-binding protein 1 - Siah-BP1
Gene names	PUF60
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	Q9UHX1

17 N-termini

5 C-termini

4 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform 2 of Poly(U)-binding-splicing factor PUF60 - Isoform 3 of Poly(U)-binding-splicing factor PUF60 - Isoform 4 of Poly(U)-binding-splicing factor PUF60 - Isoform 5 of Poly(U)-binding-splicing factor PUF60 - Isoform 6 of Poly(U)-binding-splicing factor PUF60

Sequence View

        10         20         30         40         50         60
MATATIALQV NGQQGGGSEP AAAAAVVAAG DKWKPPQGTD SIKMENGQST AAKLGLPPLT
        70         80         90        100        110        120
PEQQEALQKA KKYAMEQSIK SVLVKQTIAH QQQQLTNLQM AAVTMGFGDP LSPLQSMAAQ
       130        140        150        160        170        180
RQRALAIMCR VYVGSIYYEL GEDTIRQAFA PFGPIKSIDM SWDSVTMKHK GFAFVEYEVP
       190        200        210        220        230        240
EAAQLALEQM NSVMLGGRNI KVGRPSNIGQ AQPIIDQLAE EARAFNRIYV ASVHQDLSDD
       250        260        270        280        290        300
DIKSVFEAFG KIKSCTLARD PTTGKHKGYG FIEYEKAQSS QDAVSSMNLF DLGGQYLRVG
       310        320        330        340        350        360
KAVTPPMPLL TPATPGGLPP AAAVAAAAAT AKITAQEAVA GAAVLGTLGT PGLVSPALTL
       370        380        390        400        410        420
AQPLGTLPQA VMAAQAPGVI TGVTPARPPI PVTIPSVGVV NPILASPPTL GLLEPKKEKE
       430        440        450        460        470        480
EEELFPESER PEMLSEQEHM SISGSSARHM VMQKLLRKQE STVMVLRNMV DPKDIDDDLE
       490        500        510        520        530        540
GEVTEECGKF GAVNRVIIYQ EKQGEEEDAE IIVKIFVEFS IASETHKAIQ ALNGRWFAGR
       550
KVVAEVYDQE RFDNSDLSA         10         20         30         40         50         60
MATATIALQV NGQQGGGSEP AAAAAVVAAG DKWKPPQGTD SIKMENGQST AAKLGLPPLT
        70         80         90        100        110        120
PEQQEALQKA KKYAMEQSIK SVLVKQTIAH QQQQLTNLQM AAVTMGFGDP LSPLQSMAAQ
       130        140        150        160        170        180
RQRALAIMCR VYVGSIYYEL GEDTIRQAFA PFGPIKSIDM SWDSVTMKHK GFAFVEYEVP
       190        200        210        220        230        240
EAAQLALEQM NSVMLGGRNI KVGRPSNIGQ AQPIIDQLAE EARAFNRIYV ASVHQDLSDD
       250        260        270        280        290        300
DIKSVFEAFG KIKSCTLARD PTTGKHKGYG FIEYEKAQSS QDAVSSMNLF DLGGQYLRVG
       310        320        330        340        350        360
KAVTPPMPLL TPATPGGLPP AAAVAAAAAT AKITAQEAVA GAAVLGTLGT PGLVSPALTL
       370        380        390        400        410        420
AQPLGTLPQA VMAAQAPGVI TGVTPARPPI PVTIPSVGVV NPILASPPTL GLLEPKKEKE
       430        440        450        460        470        480
EEELFPESER PEMLSEQEHM SISGSSARHM VMQKLLRKQE STVMVLRNMV DPKDIDDDLE
       490        500        510        520        530        540
GEVTEECGKF GAVNRVIIYQ EKQGEEEDAE IIVKIFVEFS IASETHKAIQ ALNGRWFAGR
       550
KVVAEVYDQE RFDNSDLSA         10         20         30         40         50         60
MATATIALQV NGQQGGGSEP AAAAAVVAAG DKWKPPQGTD SIKMENGQST AAKLGLPPLT
        70         80         90        100        110        120
PEQQEALQKA KKYAMEQSIK SVLVKQTIAH QQQQLTNLQM AAVTMGFGDP LSPLQSMAAQ
       130        140        150        160        170        180
RQRALAIMCR VYVGSIYYEL GEDTIRQAFA PFGPIKSIDM SWDSVTMKHK GFAFVEYEVP
       190        200        210        220        230        240
EAAQLALEQM NSVMLGGRNI KVGRPSNIGQ AQPIIDQLAE EARAFNRIYV ASVHQDLSDD
       250        260        270        280        290        300
DIKSVFEAFG KIKSCTLARD PTTGKHKGYG FIEYEKAQSS QDAVSSMNLF DLGGQYLRVG
       310        320        330        340        350        360
KAVTPPMPLL TPATPGGLPP AAAVAAAAAT AKITAQEAVA GAAVLGTLGT PGLVSPALTL
       370        380        390        400        410        420
AQPLGTLPQA VMAAQAPGVI TGVTPARPPI PVTIPSVGVV NPILASPPTL GLLEPKKEKE
       430        440        450        460        470        480
EEELFPESER PEMLSEQEHM SISGSSARHM VMQKLLRKQE STVMVLRNMV DPKDIDDDLE
       490        500        510        520        530        540
GEVTEECGKF GAVNRVIIYQ EKQGEEEDAE IIVKIFVEFS IASETHKAIQ ALNGRWFAGR
       550
KVVAEVYDQE RFDNSDLSA         10         20         30         40         50         60
MATATIALQV NGQQGGGSEP AAAAAVVAAG DKWKPPQGTD SIKMENGQST AAKLGLPPLT
        70         80         90        100        110        120
PEQQEALQKA KKYAMEQSIK SVLVKQTIAH QQQQLTNLQM AAVTMGFGDP LSPLQSMAAQ
       130        140        150        160        170        180
RQRALAIMCR VYVGSIYYEL GEDTIRQAFA PFGPIKSIDM SWDSVTMKHK GFAFVEYEVP
       190        200        210        220        230        240
EAAQLALEQM NSVMLGGRNI KVGRPSNIGQ AQPIIDQLAE EARAFNRIYV ASVHQDLSDD
       250        260        270        280        290        300
DIKSVFEAFG KIKSCTLARD PTTGKHKGYG FIEYEKAQSS QDAVSSMNLF DLGGQYLRVG
       310        320        330        340        350        360
KAVTPPMPLL TPATPGGLPP AAAVAAAAAT AKITAQEAVA GAAVLGTLGT PGLVSPALTL
       370        380        390        400        410        420
AQPLGTLPQA VMAAQAPGVI TGVTPARPPI PVTIPSVGVV NPILASPPTL GLLEPKKEKE
       430        440        450        460        470        480
EEELFPESER PEMLSEQEHM SISGSSARHM VMQKLLRKQE STVMVLRNMV DPKDIDDDLE
       490        500        510        520        530        540
GEVTEECGKF GAVNRVIIYQ EKQGEEEDAE IIVKIFVEFS IASETHKAIQ ALNGRWFAGR
       550
KVVAEVYDQE RFDNSDLSA         10         20         30         40         50         60
MATATIALQV NGQQGGGSEP AAAAAVVAAG DKWKPPQGTD SIKMENGQST AAKLGLPPLT
        70         80         90        100        110        120
PEQQEALQKA KKYAMEQSIK SVLVKQTIAH QQQQLTNLQM AAVTMGFGDP LSPLQSMAAQ
       130        140        150        160        170        180
RQRALAIMCR VYVGSIYYEL GEDTIRQAFA PFGPIKSIDM SWDSVTMKHK GFAFVEYEVP
       190        200        210        220        230        240
EAAQLALEQM NSVMLGGRNI KVGRPSNIGQ AQPIIDQLAE EARAFNRIYV ASVHQDLSDD
       250        260        270        280        290        300
DIKSVFEAFG KIKSCTLARD PTTGKHKGYG FIEYEKAQSS QDAVSSMNLF DLGGQYLRVG
       310        320        330        340        350        360
KAVTPPMPLL TPATPGGLPP AAAVAAAAAT AKITAQEAVA GAAVLGTLGT PGLVSPALTL
       370        380        390        400        410        420
AQPLGTLPQA VMAAQAPGVI TGVTPARPPI PVTIPSVGVV NPILASPPTL GLLEPKKEKE
       430        440        450        460        470        480
EEELFPESER PEMLSEQEHM SISGSSARHM VMQKLLRKQE STVMVLRNMV DPKDIDDDLE
       490        500        510        520        530        540
GEVTEECGKF GAVNRVIIYQ EKQGEEEDAE IIVKIFVEFS IASETHKAIQ ALNGRWFAGR
       550
KVVAEVYDQE RFDNSDLSA

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

17 N-termini - 5 C-termini - 4 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
Q9UHX1-1-unknown	MATATI...	1	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
Q9UHX1-1-unknown	MATATI...	1	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt86643
Q9UHX1-10-unknown	VNGQQG...	10	inferred from electronic annotation	unknown	TISdb		inferred from TISdb
Q9UHX1-10-unknown	VNGQQG...	10	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt194881
Q9UHX1-12-	GQQGGG...	12		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q9UHX1-12-unknown	GQQGGG...	12	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175127
Q9UHX1-41-unknown	SIKMEN...	41	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168406
Q9UHX1-41-unknown	SIKMEN...	41	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168407
Q9UHX1-41-unknown	SIKMEN...	41	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168408
Q9UHX1-41-	SIKMEN...	41		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
Q9UHX1-41-	SIKMEN...	41		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
Q9UHX1-44-unknown	MENGQS...	44	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt86644
Q9UHX1-44-unknown	MENGQS...	44	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt86645
Q9UHX1-44-unknown	MENGQS...	44	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt102532
Q9UHX1-44-unknown	MENGQS...	44	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt102533
Q9UHX1-44-unknown	MENGQS...	44	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt102534
Q9UHX1-210-unknown	QAQPII...	210	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6849
Q9UHX1-210-unknown	QAQPII...	210	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt144747
Q9UHX1-210-unknown	QAQPII...	210	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt145317
Q9UHX1-210-unknown	QAQPII...	210	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt145425
Q9UHX1-210-unknown	QAQPII...	210	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt145944
Q9UHX1-210-unknown	QAQPII...	210	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt146366
Q9UHX1-232-	SVHQDL...	232		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q9UHX1-232-unknown	SVHQDL...	232	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6855
Q9UHX1-232-unknown	SVHQDL...	232	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC8137
Q9UHX1-232-unknown	SVHQDL...	232	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt145608
Q9UHX1-232-unknown	SVHQDL...	232	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt146114
Q9UHX1-232-unknown	SVHQDL...	232	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt146226
Q9UHX1-232-unknown	SVHQDL...	232	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt146654
Q9UHX1-232-unknown	SVHQDL...	232	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt147179
Q9UHX1-399-unknown	VVNPIL...	399	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168412
Q9UHX1-399-unknown	VVNPIL...	399	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168410
Q9UHX1-399-unknown	VVNPIL...	399	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168413
Q9UHX1-399-unknown	VVNPIL...	399	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168411
Q9UHX1-399-unknown	VVNPIL...	399	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt168414
Q9UHX1-399-	VVNPIL...	399		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
Q9UHX1-399-	VVNPIL...	399		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
Q9UHX1-399-	VVNPIL...	399		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q9UHX1-399-	VVNPIL...	399		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
Q9UHX1-405-	ASPPTL...	405		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
Q9UHX1-405-unknown	ASPPTL...	405	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175131
Q9UHX1-405-unknown	ASPPTL...	405	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175129
Q9UHX1-405-unknown	ASPPTL...	405	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175132
Q9UHX1-405-unknown	ASPPTL...	405	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175130
Q9UHX1-405-unknown	ASPPTL...	405	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175133
Q9UHX1-406-unknown	SPPTLG...	406	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18161
Q9UHX1-406-unknown	SPPTLG...	406	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt150878
Q9UHX1-406-unknown	SPPTLG...	406	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt151183
Q9UHX1-406-unknown	SPPTLG...	406	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt151262
Q9UHX1-406-unknown	SPPTLG...	406	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt151565
Q9UHX1-406-unknown	SPPTLG...	406	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt151831
Q9UHX1-435-unknown	SEQEHM...	435		unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
Q9UHX1-435-unknown	SEQEHM...	435	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt160087
Q9UHX1-435-unknown	SEQEHM...	435	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt160085
Q9UHX1-435-unknown	SEQEHM...	435	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt160088
Q9UHX1-435-unknown	SEQEHM...	435	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt160086
Q9UHX1-435-unknown	SEQEHM...	435	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt160089
Q9UHX1-435-	SEQEHM...	435		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...PSNIGQ	209	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6849
...PSNIGQ	209	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt128022
...PSNIGQ	209	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt128608
...PSNIGQ	209	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt128718
...PSNIGQ	209	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt129241
...PSNIGQ	209	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt129670
...RIYVAS	231	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC6855
...RIYVAS	231	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC8137
...RIYVAS	231	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt128906
...RIYVAS	231	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt129414
...RIYVAS	231	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt129530
...RIYVAS	231	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt129963
...RIYVAS	231	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt130519
...NPILAS	405	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18161
...NPILAS	405	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt134280
...NPILAS	405	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt134585
...NPILAS	405	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt134663
...NPILAS	405	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt134970
...NPILAS	405	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt135236
...SDLSA	559		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...SDLSA	559		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...SDLSA	559	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...SDLSA	559	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt82263
...SDLSA	559	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt82265
...SDLSA	559	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt82262
...SDLSA	559	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt82264
...SDLSA	559	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt82261
...SDLSA	559		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...SDLSA	559		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CATL1_HUMAN	209	NIGQ.\|.QAQP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	231	YVAS.\|.SVHQ	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	231	YVAS.\|.SVHQ	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1A_HUMAN	405	ILAS.\|.SPPT	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)