O15446: DNA-directed RNA polymerase I subunit RPA34

General Information

Protein names	- DNA-directed RNA polymerase I subunit RPA34 - A34.5 - Antisense to ERCC-1 protein - ASE-1 - CD3-epsilon-associated protein - CAST - CD3E-associated protein - RNA polymerase I-associated factor PAF49
Gene names	CD3EAP
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	O15446

10 N-termini

10 C-termini

8 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MEEPQAGDAA RFSCPPNFTA KPPASESPRF SLEALTGPDT ELWLIQAPAD FAPECFNGRH
        70         80         90        100        110        120
VPLSGSQIVK GKLAGKRHRY RVLSSCPQAG EATLLAPSTE AGGGLTCASA PQGTLRILEG
       130        140        150        160        170        180
PQQSLSGSPL QPIPASPPPQ IPPGLRPRFC AFGGNPPVTG PRSALAPNLL TSGKKKKEMQ
       190        200        210        220        230        240
VTEAPVTQEA VNGHGALEVD MALGSPEMDV RKKKKKKNQQ LKEPEAAGPV GTEPTVETLE
       250        260        270        280        290        300
PLGVLFPSTT KKRKKPKGKE TFEPEDKTVK QEQINTEPLE DTVLSPTKKR KRQKGTEGME
       310        320        330        340        350        360
PEEGVTVESQ PQVKVEPLEE AIPLPPTKKR KKEKGQMAMM EPGTEAMEPV EPEMKPLESP
       370        380        390        400        410        420
GGTMAPQQPE GAKPQAQAAL AAPKKKTKKE KQQDATVEPE TEVVGPELPD DLEPQAAPTS
       430        440        450        460        470        480
TKKKKKKKER GHTVTEPIQP LEPELPGEGQ PEARATPGST KKRKKQSQES RMPETVPQEE
       490        500        510
MPGPPLNSES GEEAPTGRDK KRKQQQQQPV

Isoforms

- Isoform 2 of DNA-directed RNA polymerase I subunit RPA34

Sequence View

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Protein Neighborhood

Domains & Features

10 N-termini - 10 C-termini - 8 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
O15446-1-Acetylation	MEEPQA...	1	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
O15446-1-Acetylation	MEEPQA...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
O15446-1-Acetylation	MEEPQA...	1	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
O15446-20-unknown	AKPPAS...	20		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5146
O15446-20-unknown	AKPPAS...	20		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt136969
O15446-40-unknown	TELWLI...	40		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10713
O15446-40-unknown	TELWLI...	40		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt138537
O15446-64-unknown	SGSQIV...	64		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13727
O15446-64-unknown	SGSQIV...	64		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt140377
O15446-202-	ALGSPE...	202			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
O15446-202-	ALGSPE...	202			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
O15446-202-	ALGSPE...	202			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_CD95	23264352
O15446-202-	ALGSPE...	202			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
O15446-202-	ALGSPE...	202			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
O15446-202-	ALGSPE...	202			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O15446-202-	ALGSPE...	202			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
O15446-202-	ALGSPE...	202			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
O15446-202-	ALGSPE...	202			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
O15446-202-	ALGSPE...	202			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
O15446-202-unknown	ALGSPE...	202		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10715
O15446-202-unknown	ALGSPE...	202			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
O15446-202-unknown	ALGSPE...	202		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt146685
O15446-232-unknown	TEPTVE...	232		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC16872
O15446-232-unknown	TEPTVE...	232		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt147892
O15446-243-unknown	GVLFPS...	243		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13729
O15446-243-unknown	GVLFPS...	243		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt148272
O15446-401-unknown	TEVVGP...	401		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10717
O15446-401-unknown	TEVVGP...	401		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt152043
O15446-416-unknown	AAPTST...	416		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13731
O15446-416-unknown	AAPTST...	416		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt152307

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...PPNFTA	19	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5146
...LTGPDT	39	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10713
...LTGPDT	39	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt121585
...RHVPLS	63	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13727
...RHVPLS	63	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt123500
...LEVDMA	201	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10715
...LEVDMA	201	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt129995
...AGPVGT	231	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC16872
...AGPVGT	231	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt131252
...TLEPLG	242	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13729
...TLEPLG	242	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt131642
...TVEPET	400	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10717
...TVEPET	400	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt135453
...DLEPQA	415	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC13731
...DLEPQA	415	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt135718
...QQQPV	510	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...QQQPV	510	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TCt83720
...QQQPV	510		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...QQQPV	510		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CATL1_HUMAN	19	NFTA.\|.AKPP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
GRAB_HUMAN	39	GPDT.\|.TELW	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAM_HUMAN	63	VPLS.\|.SGSQ	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,
GRAB_HUMAN	201	VDMA.\|.ALGS	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
MEP1A_HUMAN	231	PVGT.\|.TEPT	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
GRAM_HUMAN	242	EPLG.\|.GVLF	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,
GRAB_HUMAN	400	EPET.\|.TEVV	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,
GRAM_HUMAN	415	EPQA.\|.AAPT	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)