O43707: Alpha-actinin-4 {ECO:0000305}

General Information

Protein names	- Alpha-actinin-4 {ECO:0000305} - Non-muscle alpha-actinin 4 {ECO:0000303\|PubMed:10656685}
Gene names	ACTN4
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	O43707

29 N-termini

19 C-termini

19 Cleavages

0 Substrates

Sequence

Isoforms

- Isoform ACTN4ISO of Alpha-actinin-4 - Isoform 3 of Alpha-actinin-4

Sequence View

        10         20         30         40         50         60
MVDYHAANQS YQYGPSSAGN GAGGGGSMGD YMAQEDDWDR DLLLDPAWEK QQRKTFTAWC
        70         80         90        100        110        120
NSHLRKAGTQ IENIDEDFRD GLKLMLLLEV ISGERLPKPE RGKMRVHKIN NVNKALDFIA
       130        140        150        160        170        180
SKGVKLVSIG AEEIVDGNAK MTLGMIWTII LRFAIQDISV EETSAKEGLL LWCQRKTAPY
       190        200        210        220        230        240
KNVNVQNFHI SWKDGLAFNA LIHRHRPELI EYDKLRKDDP VTNLNNAFEV AEKYLDIPKM
       250        260        270        280        290        300
LDAEDIVNTA RPDEKAIMTY VSSFYHAFSG AQKAETAANR ICKVLAVNQE NEHLMEDYEK
       310        320        330        340        350        360
LASDLLEWIR RTIPWLEDRV PQKTIQEMQQ KLEDFRDYRR VHKPPKVQEK CQLEINFNTL
       370        380        390        400        410        420
QTKLRLSNRP AFMPSEGKMV SDINNGWQHL EQAEKGYEEW LLNEIRRLER LDHLAEKFRQ
       430        440        450        460        470        480
KASIHEAWTD GKEAMLKHRD YETATLSDIK ALIRKHEAFE SDLAAHQDRV EQIAAIAQEL
       490        500        510        520        530        540
NELDYYDSHN VNTRCQKICD QWDALGSLTH SRREALEKTE KQLEAIDQLH LEYAKRAAPF
       550        560        570        580        590        600
NNWMESAMED LQDMFIVHTI EEIEGLISAH DQFKSTLPDA DREREAILAI HKEAQRIAES
       610        620        630        640        650        660
NHIKLSGSNP YTTVTPQIIN SKWEKVQQLV PKRDHALLEE QSKQQSNEHL RRQFASQANV
       670        680        690        700        710        720
VGPWIQTKME EIGRISIEMN GTLEDQLSHL KQYERSIVDY KPNLDLLEQQ HQLIQEALIF
       730        740        750        760        770        780
DNKHTNYTME HIRVGWEQLL TTIARTINEV ENQILTRDAK GISQEQMQEF RASFNHFDKD
       790        800        810        820        830        840
HGGALGPEEF KACLISLGYD VENDRQGEAE FNRIMSLVDP NHSGLVTFQA FIDFMSRETT
       850        860        870        880        890        900
DTDTADQVIA SFKVLAGDKN FITAEELRRE LPPDQAEYCI ARMAPYQGPD AVPGALDYKS
       910
FSTALYGESD L         10         20         30         40         50         60
MVDYHAANQS YQYGPSSAGN GAGGGGSMGD YMAQEDDWDR DLLLDPAWEK QQRKTFTAWC
        70         80         90        100        110        120
NSHLRKAGTQ IENIDEDFRD GLKLMLLLEV ISGERLPKPE RGKMRVHKIN NVNKALDFIA
       130        140        150        160        170        180
SKGVKLVSIG AEEIVDGNAK MTLGMIWTII LRFAIQDISV EETSAKEGLL LWCQRKTAPY
       190        200        210        220        230        240
KNVNVQNFHI SWKDGLAFNA LIHRHRPELI EYDKLRKDDP VTNLNNAFEV AEKYLDIPKM
       250        260        270        280        290        300
LDAEDIVNTA RPDEKAIMTY VSSFYHAFSG AQKAETAANR ICKVLAVNQE NEHLMEDYEK
       310        320        330        340        350        360
LASDLLEWIR RTIPWLEDRV PQKTIQEMQQ KLEDFRDYRR VHKPPKVQEK CQLEINFNTL
       370        380        390        400        410        420
QTKLRLSNRP AFMPSEGKMV SDINNGWQHL EQAEKGYEEW LLNEIRRLER LDHLAEKFRQ
       430        440        450        460        470        480
KASIHEAWTD GKEAMLKHRD YETATLSDIK ALIRKHEAFE SDLAAHQDRV EQIAAIAQEL
       490        500        510        520        530        540
NELDYYDSHN VNTRCQKICD QWDALGSLTH SRREALEKTE KQLEAIDQLH LEYAKRAAPF
       550        560        570        580        590        600
NNWMESAMED LQDMFIVHTI EEIEGLISAH DQFKSTLPDA DREREAILAI HKEAQRIAES
       610        620        630        640        650        660
NHIKLSGSNP YTTVTPQIIN SKWEKVQQLV PKRDHALLEE QSKQQSNEHL RRQFASQANV
       670        680        690        700        710        720
VGPWIQTKME EIGRISIEMN GTLEDQLSHL KQYERSIVDY KPNLDLLEQQ HQLIQEALIF
       730        740        750        760        770        780
DNKHTNYTME HIRVGWEQLL TTIARTINEV ENQILTRDAK GISQEQMQEF RASFNHFDKD
       790        800        810        820        830        840
HGGALGPEEF KACLISLGYD VENDRQGEAE FNRIMSLVDP NHSGLVTFQA FIDFMSRETT
       850        860        870        880        890        900
DTDTADQVIA SFKVLAGDKN FITAEELRRE LPPDQAEYCI ARMAPYQGPD AVPGALDYKS
       910
FSTALYGESD L

Filter Information:

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Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

29 N-termini - 19 C-termini - 19 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
O43707-1-unknown	MVDYHA...	1	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
O43707-1-unknown	MVDYHA...	1	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt66237
O43707-1-unknown	MVDYHA...	1	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt66238
O43707-21-	GAGGGG...	21		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
O43707-21-unknown	GAGGGG...	21	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt176667
O43707-21-unknown	GAGGGG...	21	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt176668
O43707-25-unknown	GGSMGD...	25	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175019
O43707-25-unknown	GGSMGD...	25	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175020
O43707-25-	GGSMGD...	25		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
O43707-25-	GGSMGD...	25		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
O43707-27-	SMGDYM...	27		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
O43707-27-unknown	SMGDYM...	27	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt176670
O43707-27-unknown	SMGDYM...	27	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt176671
O43707-31-unknown	YMAQED...	31	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175022
O43707-31-unknown	YMAQED...	31	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt175023
O43707-31-	YMAQED...	31		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
O43707-31-	YMAQED...	31		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
O43707-38-unknown	WDRDLL...	38	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2396
O43707-38-unknown	WDRDLL...	38	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt138173
O43707-40-unknown	RDLLLD...	40	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2399
O43707-40-unknown	RDLLLD...	40	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt138324
O43707-78-unknown	FRDGLK...	78	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2402
O43707-129-unknown	IGAEEI...	129	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5175
O43707-129-unknown	IGAEEI...	129	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7022
O43707-214-unknown	KLRKDD...	214	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2404
O43707-226-unknown	NAFEVA...	226	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7023
O43707-245-unknown	DIVNTA...	245	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18239
O43707-355-unknown	INFNTL...	355	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7024
O43707-355-unknown	INFNTL...	355	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt144127
O43707-461-unknown	SDLAAH...	461	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5176
O43707-461-unknown	SDLAAH...	461	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt148174
O43707-463-unknown	LAAHQD...	463	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2405
O43707-463-unknown	LAAHQD...	463	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt148243
O43707-465-unknown	AHQDRV...	465	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5178
O43707-465-unknown	AHQDRV...	465	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt148285
O43707-475-unknown	AIAQEL...	475	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt174064
O43707-475-	AIAQEL...	475		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
O43707-485-unknown	YYDSHN...	485	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2407
O43707-485-unknown	YYDSHN...	485	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt149015
O43707-485-unknown	YYDSHN...	485	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt198590
O43707-503-unknown	DALGSL...	503	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18240
O43707-503-unknown	DALGSL...	503	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt143112
O43707-503-unknown	DALGSL...	503	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt149445
O43707-504-unknown	ALGSLT...	504	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2409
O43707-504-unknown	ALGSLT...	504	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt143171
O43707-504-unknown	ALGSLT...	504	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt149469
O43707-779-unknown	KDHGGA...	779	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5180
O43707-779-unknown	KDHGGA...	779	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt151813
O43707-779-unknown	KDHGGA...	779	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt154109
O43707-805-unknown	RQGEAE...	805	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2412
O43707-805-unknown	RQGEAE...	805	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt152250
O43707-805-unknown	RQGEAE...	805	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt154408
O43707-844-	TADQVI...	844		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
O43707-844-unknown	TADQVI...	844	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2415
O43707-844-unknown	TADQVI...	844	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt152830
O43707-844-unknown	TADQVI...	844	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt154965

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...AQEDDW	37	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2396
...AQEDDW	37	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt121200
...AQEDDW	37	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt121201
...EDDWDR	39	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2399
...EDDWDR	39	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt121361
...EDDWDR	39	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt121362
...NIDEDF	77	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2402
...NIDEDF	77	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt124339
...VKLVSI	128	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5175
...VKLVSI	128	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7022
...LIEYDK	213	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2404
...VTNLNN	225	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7023
...MLDAED	244	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18239
...KCQLEI	354	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7024
...KCQLEI	354	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt127378
...HEAFES	460	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5176
...HEAFES	460	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt131541
...AFESDL	462	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2405
...AFESDL	462	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt131610
...ESDLAA	464	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5178
...ESDLAA	464	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt131655
...LNELDY	484	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2407
...LNELDY	484	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt132398
...ICDQWD	502	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18240
...ICDQWD	502	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt126333
...ICDQWD	502	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt132830
...CDQWDA	503	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2409
...CDQWDA	503	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt126396
...CDQWDA	503	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt132854
...FNHFDK	778	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5180
...FNHFDK	778	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt135217
...FNHFDK	778	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt137529
...DVENDR	804	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2412
...DVENDR	804	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt135660
...DVENDR	804	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt137829
...TTDTDT	843	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2415
...TTDTDT	843	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt136255
...TTDTDT	843	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt138392
...YGESDL	910	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2418
...YGESDL	910	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt137057
...YGESDL	910	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt139227
...GESDL	911	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...GESDL	911	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt61856
...GESDL	911	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt61855

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CASP6_HUMAN	37	EDDW.\|.WDRD	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CASP6_HUMAN	39	DWDR.\|.RDLL	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CASP6_HUMAN	77	DEDF.\|.FRDG	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CATL1_HUMAN	128	LVSI.\|.IGAE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	128	LVSI.\|.IGAE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CASP6_HUMAN	213	EYDK.\|.KLRK	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CATS_HUMAN	225	NLNN.\|.NAFE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	244	DAED.\|.DIVN	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATS_HUMAN	354	QLEI.\|.INFN	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	460	AFES.\|.SDLA	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CASP6_HUMAN	462	ESDL.\|.LAAH	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CATL1_HUMAN	464	DLAA.\|.AHQD	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CASP6_HUMAN	484	ELDY.\|.YYDS	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
MEP1B_HUMAN	502	DQWD.\|.DALG	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CASP6_HUMAN	503	QWDA.\|.ALGS	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CATL1_HUMAN	778	HFDK.\|.KDHG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CASP6_HUMAN	804	ENDR.\|.RQGE	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CASP6_HUMAN	843	DTDT.\|.TADQ	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
CASP6_HUMAN	910	ESDL.\|.L	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)