P30101: Protein disulfide-isomerase A3

General Information

Protein names	- Protein disulfide-isomerase A3 - 5.3.4.1 - 58 kDa glucose-regulated protein - 58 kDa microsomal protein - p58 - Disulfide isomerase ER-60 - Endoplasmic reticulum resident protein 57 - ER protein 57 - ERp57 - Endoplasmic reticulum resident protein 60 - ER protein 60 - ERp60
Gene names	PDIA3
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P30101

14 N-termini

7 C-termini

6 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MRLRRLALFP GVALLLAAAR LAAASDVLEL TDDNFESRIS DTGSAGLMLV EFFAPWCGHC
        70         80         90        100        110        120
KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY PTLKIFRDGE EAGAYDGPRT
       130        140        150        160        170        180
ADGIVSHLKK QAGPASVPLR TEEEFKKFIS DKDASIVGFF DDSFSEAHSE FLKAASNLRD
       190        200        210        220        230        240
NYRFAHTNVE SLVNEYDDNG EGIILFRPSH LTNKFEDKTV AYTEQKMTSG KIKKFIQENI
       250        260        270        280        290        300
FGICPHMTED NKDLIQGKDL LIAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA
       310        320        330        340        350        360
VASRKTFSHE LSDFGLESTA GEIPVVAIRT AKGEKFVMQE EFSRDGKALE RFLQDYFDGN
       370        380        390        400        410        420
LKRYLKSEPI PESNDGPVKV VVAENFDEIV NNENKDVLIE FYAPWCGHCK NLEPKYKELG
       430        440        450        460        470        480
EKLSKDPNIV IAKMDATAND VPSPYEVRGF PTIYFSPANK KLNPKKYEGG RELSDFISYL
       490        500
QREATNPPVI QEEKPKKKKK AQEDL

Isoforms

Sequence View

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Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

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Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

14 N-termini - 7 C-termini - 6 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P30101-22-	AAASDV...	22		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P30101-24-	ASDVLE...	24		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P30101-24-	ASDVLE...	24		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P30101-24-	ASDVLE...	24		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P30101-24-	ASDVLE...	24		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P30101-24-	ASDVLE...	24		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P30101-25-	SDVLEL...	25		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P30101-25-	SDVLEL...	25		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P30101-25-	SDVLEL...	25		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P30101-25-	SDVLEL...	25		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P30101-25-	SDVLEL...	25		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P30101-25-	SDVLEL...	25		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P30101-25-	SDVLEL...	25		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P30101-25-unknown	SDVLEL...	25	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P30101-163-unknown	SFSEAH...	163	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1215
P30101-166-unknown	EAHSEF...	166	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5772
P30101-166-unknown	EAHSEF...	166	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7413
P30101-208-	PSHLTN...	208		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P30101-272-unknown	NAKGSN...	272		unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P30101-272-	NAKGSN...	272		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P30101-272-	NAKGSN...	272		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P30101-318-unknown	STAGEI...	318	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7414
P30101-355-unknown	DYFDGN...	355	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19025
P30101-384-unknown	ENFDEI...	384	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7415
P30101-499-	KKAQED...	499		Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P30101-500-	KAQEDL...	500		Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P30101-500-	KAQEDL...	500		Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P30101-500-	KAQEDL...	500		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P30101-500-	KAQEDL...	500		Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...GFFDDS	162	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1215
...DDSFSE	165	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5772
...DDSFSE	165	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7413
...DFGLES	317	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7414
...ERFLQD	354	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19025
...KVVVAE	383	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7415
...AQEDL	505		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...AQEDL	505		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...AQEDL	505	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...AQEDL	505		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...AQEDL	505		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CASP1_HUMAN	162	FDDS.\|.SFSE	inferred from experiment	unknown	MEROPS	Wells JA	Agard NJ et al.:Inflammatory stimuli regulate c... (C14.001)	20173201,
CATL1_HUMAN	165	SFSE.\|.EAHS	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	165	SFSE.\|.EAHS	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	317	GLES.\|.STAG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	354	FLQD.\|.DYFD	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATS_HUMAN	383	VVAE.\|.ENFD	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)