P33991: DNA replication licensing factor MCM4

General Information

Protein names	- DNA replication licensing factor MCM4 - 3.6.4.12 - CDC21 homolog - P1-CDC21
Gene names	MCM4
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P33991

20 N-termini

10 C-termini

6 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MSSPASTPSR RGSRRGRATP AQTPRSEDAR SSPSQRRRGE DSTSTGELQP MPTSPGVDLQ
        70         80         90        100        110        120
SPAAQDVLFS SPPQMHSSAI PLDFDVSSPL TYGTPSSRVE GTPRSGVRGT PVRQRPDLGS
       130        140        150        160        170        180
AQKGLQVDLQ SDGAAAEDIV ASEQSLGQKL VIWGTDVNVA ACKENFQRFL QRFIDPLAKE
       190        200        210        220        230        240
EENVGIDITE PLYMQRLGEI NVIGEPFLNV NCEHIKSFDK NLYRQLISYP QEVIPTFDMA
       250        260        270        280        290        300
VNEIFFDRYP DSILEHQIQV RPFNALKTKN MRNLNPEDID QLITISGMVI RTSQLIPEMQ
       310        320        330        340        350        360
EAFFQCQVCA HTTRVEMDRG RIAEPSVCGR CHTTHSMALI HNRSLFSDKQ MIKLQESPED
       370        380        390        400        410        420
MPAGQTPHTV ILFAHNDLVD KVQPGDRVNV TGIYRAVPIR VNPRVSNVKS VYKTHIDVIH
       430        440        450        460        470        480
YRKTDAKRLH GLDEEAEQKL FSEKRVELLK ELSRKPDIYE RLASALAPSI YEHEDIKKGI
       490        500        510        520        530        540
LLQLFGGTRK DFSHTGRGKF RAEINILLCG DPGTSKSQLL QYVYNLVPRG QYTSGKGSSA
       550        560        570        580        590        600
VGLTAYVMKD PETRQLVLQT GALVLSDNGI CCIDEFDKMN ESTRSVLHEV MEQQTLSIAK
       610        620        630        640        650        660
AGIICQLNAR TSVLAAANPI ESQWNPKKTT IENIQLPHTL LSRFDLIFLL LDPQDEAYDR
       670        680        690        700        710        720
RLAHHLVALY YQSEEQAEEE LLDMAVLKDY IAYAHSTIMP RLSEEASQAL IEAYVDMRKI
       730        740        750        760        770        780
GSSRGMVSAY PRQLESLIRL AEAHAKVRLS NKVEAIDVEE AKRLHREALK QSATDPRTGI
       790        800        810        820        830        840
VDISILTTGM SATSRKRKEE LAEALKKLIL SKGKTPALKY QQLFEDIRGQ SDIAITKDMF
       850        860
EEALRALADD DFLTVTGKTV RLL

Isoforms

Sequence View

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Evidence Codes:

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Evidence Names:

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Protein Neighborhood

Domains & Features

20 N-termini - 10 C-termini - 6 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P33991-2-unknown	MSSPAS...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P33991-2-Acetylation	SSPAST...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P33991-2-Acetylation	SSPAST...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P33991-2-Acetylation	SSPAST...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P33991-51-unknown	MPTSPG...	51		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000428833
P33991-70-	SSPPQM...	70			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P33991-70-	SSPPQM...	70			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P33991-79-	AIPLDF...	79			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P33991-79-	AIPLDF...	79			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P33991-83-unknown	DFDVSS...	83		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC14086
P33991-86-unknown	VSSPLT...	86		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11447
P33991-93-	GTPSSR...	93			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P33991-124-	GLQVDL...	124			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	RMPI_untreated	23264352
P33991-133-	GAAAED...	133			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P33991-133-unknown	GAAAED...	133		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1217
P33991-133-unknown	GAAAED...	133			unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P33991-133-unknown	GAAAED...	133			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P33991-134-	AAAEDI...	134			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P33991-135-	AAEDIV...	135			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P33991-135-	AAEDIV...	135			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P33991-135-	AAEDIV...	135			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P33991-135-	AAEDIV...	135			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P33991-135-	AAEDIV...	135			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P33991-188-unknown	ITEPLY...	188		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11448
P33991-239-unknown	MAVNEI...	239		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000427875
P33991-273-	NLNPED...	273			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P33991-396-	AVPIRV...	396			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P33991-396-	AVPIRV...	396			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P33991-568-	NGICCI...	568			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P33991-568-	NGICCI...	568			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P33991-568-	NGICCI...	568			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P33991-568-	NGICCI...	568			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P33991-617-unknown	ANPIES...	617		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3772
P33991-846-unknown	ALADDD...	846		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5807

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...SAIPLD	82	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC14086
...PLDFDV	85	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11447
...DLQSDG	132	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC1217
...AEDIVA	140	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000430329
...NVGIDI	187	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11448
...QIQVRP	261	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000428833
...LVLQTG	560	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000427875
...SVLAAA	616	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3772
...EEALRA	845	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5807
...TVRLL	863	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAM_HUMAN	82	IPLD.\|.DFDV	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,
GRAB_HUMAN	85	DFDV.\|.VSSP	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,
CASP1_HUMAN	132	QSDG.\|.GAAA	inferred from experiment	unknown	MEROPS	Wells JA	Agard NJ et al.:Inflammatory stimuli regulate c... (C14.001)	20173201,
GRAB_HUMAN	187	GIDI.\|.ITEP	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
CATB_HUMAN	616	LAAA.\|.ANPI	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	845	ALRA.\|.ALAD	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)