P38919: Eukaryotic initiation factor 4A-III

General Information

Protein names	- Eukaryotic initiation factor 4A-III - eIF-4A-III - eIF4A-III - 3.6.4.13 {ECO:0000269\|PubMed:16170325, ECO:0000269\|PubMed:17375189, ECO:0000269\|PubMed:22961380} - ATP-dependent RNA helicase DDX48 - ATP-dependent RNA helicase eIF4A-3 - DEAD box protein 48 - Eukaryotic initiation factor 4A-like NUK-34 - Eukaryotic translation initiation factor 4A isoform 3 - Nuclear matrix protein 265 - NMP 265 - hNMP 265 - Eukaryotic initiation factor 4A-III, N-terminally processed
Gene names	EIF4A3
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P38919

10 N-termini

8 C-termini

6 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MATTATMATS GSARKRLLKE EDMTKVEFET SEEVDVTPTF DTMGLREDLL RGIYAYGFEK
        70         80         90        100        110        120
PSAIQQRAIK QIIKGRDVIA QSQSGTGKTA TFSISVLQCL DIQVRETQAL ILAPTRELAV
       130        140        150        160        170        180
QIQKGLLALG DYMNVQCHAC IGGTNVGEDI RKLDYGQHVV AGTPGRVFDM IRRRSLRTRA
       190        200        210        220        230        240
IKMLVLDEAD EMLNKGFKEQ IYDVYRYLPP ATQVVLISAT LPHEILEMTN KFMTDPIRIL
       250        260        270        280        290        300
VKRDELTLEG IKQFFVAVER EEWKFDTLCD LYDTLTITQA VIFCNTKRKV DWLTEKMREA
       310        320        330        340        350        360
NFTVSSMHGD MPQKERESIM KEFRSGASRV LISTDVWARG LDVPQVSLII NYDLPNNREL
       370        380        390        400        410
YIHRIGRSGR YGRKGVAINF VKNDDIRILR DIEQYYSTQI DEMPMNVADL I

Isoforms

Sequence View

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Protein Neighborhood

Domains & Features

10 N-termini - 8 C-termini - 6 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P38919-1-Acetylation	MATTAT...	1	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P38919-2-Acetylation	ATTATM...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P38919-2-Acetylation	ATTATM...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P38919-2-Acetylation	ATTATM...	2	acetylation-		other		CNRS/ISV	Large_scale_NTA_Human
P38919-2-Acetylation	ATTATM...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P38919-2-Acetylation	ATTATM...	2	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P38919-17-	LLKEED...	17			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P38919-30-unknown	TSEEVD...	30		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11480
P38919-58-unknown	FEKPSA...	58		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5828
P38919-59-Pyrrolidone carboxylic acid (Glu)	EKPSAI...	59	pyrrolidone carboxylic acid-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P38919-60-unknown	KPSAIQ...	60		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5829
P38919-141-unknown	IGGTNV...	141		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3786
P38919-183-	MLVLDE...	183			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P38919-183-	MLVLDE...	183			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P38919-306-unknown	SMHGDM...	306		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7453
P38919-306-unknown	SMHGDM...	306		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5830

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
...KVEFET	29	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11480
...IYAYGF	57	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5828
...AYGFEK	59	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5829
...QCHACI	140	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3786
...GGTNVG	146	inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000460439
...NFTVSS	305	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7453
...NFTVSS	305	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5830
...VADLI	411		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...VADLI	411		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,
...VADLI	411	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
...VADLI	411		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345,
...VADLI	411		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345,

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAB_HUMAN	29	EFET.\|.TSEE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,
CATL1_HUMAN	57	AYGF.\|.FEKP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	59	GFEK.\|.KPSA	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	140	HACI.\|.IGGT	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	305	TVSS.\|.SMHG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	305	TVSS.\|.SMHG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)