P69905: Hemoglobin subunit alpha

General Information

Protein names	- Hemoglobin subunit alpha - Alpha-globin - Hemoglobin alpha chain
Gene names	HBA2
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P69905

81 N-termini

13 C-termini

13 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MVLSPADKTN VKAAWGKVGA HAGEYGAEAL ERMFLSFPTT KTYFPHFDLS HGSAQVKGHG
        70         80         90        100        110        120
KKVADALTNA VAHVDDMPNA LSALSDLHAH KLRVDPVNFK LLSHCLLVTL AAHLPAEFTP
       130        140
AVHASLDKFL ASVSTVLTSK YR

Isoforms

Sequence View

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

81 N-termini - 13 C-termini - 13 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P69905-1-	MVLSPA...	1			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P69905-1-	MVLSPA...	1			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-1-acetylation	MVLSPA...	1	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-2-unknown	VLSPAD...	2		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P69905-2-unknown	VLSPAD...	2			unknown			Wildes D. et al: Sampling the N-terminal proteome of human blood	20173099
P69905-2-	VLSPAD...	2			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P69905-2-	VLSPAD...	2			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-2-acetylation	VLSPAD...	2	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-3-	LSPADK...	3			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-4-	SPADKT...	4			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-6-acetylation	ADKTNV...	6	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-8-	KTNVKA...	8			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-9-	TNVKAA...	9			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-9-acetylation	TNVKAA...	9	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-10-	NVKAAW...	10			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-11-unknown	VKAAWG...	11		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC16754
P69905-11-	VKAAWG...	11			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-11-acetylation	VKAAWG...	11	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-13-	AAWGKV...	13			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-13-acetylation	AAWGKV...	13	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-14-	AWGKVG...	14			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-15-	WGKVGA...	15			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-16-	GKVGAH...	16			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-16-acetylation	GKVGAH...	16	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-17-	KVGAHA...	17			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-17-acetylation	KVGAHA...	17	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-18-	VGAHAG...	18			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-18-acetylation	VGAHAG...	18	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-19-	GAHAGE...	19			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-19-acetylation	GAHAGE...	19	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-20-	AHAGEY...	20			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P69905-20-	AHAGEY...	20			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P69905-20-	AHAGEY...	20			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-20-acetylation	AHAGEY...	20	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-21-	HAGEYG...	21			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-21-acetylation	HAGEYG...	21	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-22-	AGEYGA...	22			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-22-acetylation	AGEYGA...	22	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-23-	GEYGAE...	23			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-31-unknown	ERMFLS...	31		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC4607
P69905-33-unknown	MFLSFP...	33		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000380899
P69905-33-unknown	MFLSFP...	33		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000380908
P69905-33-unknown	MFLSFP...	33			unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P69905-33-	MFLSFP...	33			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P69905-33-	MFLSFP...	33			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P69905-33-	MFLSFP...	33			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P69905-33-	MFLSFP...	33			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P69905-33-	MFLSFP...	33			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P69905-33-	MFLSFP...	33			Subtiligase Based Positive Selection		Wells	RMPI_untreated	23264352
P69905-33-	MFLSFP...	33			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P69905-34-	FLSFPT...	34			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P69905-35-unknown	LSFPTT...	35		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC4608
P69905-38-unknown	PTTKTY...	38		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC4609
P69905-42-	TYFPHF...	42			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P69905-42-	TYFPHF...	42			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P69905-46-	HFDLSH...	46			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P69905-60-	GKKVAD...	60			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-60-acetylation	GKKVAD...	60	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-61-	KKVADA...	61			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-62-	KVADAL...	62			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-62-acetylation	KVADAL...	62	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-63-	VADALT...	63			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-63-acetylation	VADALT...	63	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-64-	ADALTN...	64			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-65-	DALTNA...	65			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-65-acetylation	DALTNA...	65	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-66-	ALTNAV...	66			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-66-acetylation	ALTNAV...	66	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-68-	TNAVAH...	68			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-68-acetylation	TNAVAH...	68	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-69-	NAVAHV...	69			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-70-unknown	AVAHVD...	70		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC16755
P69905-70-	AVAHVD...	70			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-70-acetylation	AVAHVD...	70	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-73-	HVDDMP...	73			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-74-	VDDMPN...	74			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-80-	ALSALS...	80			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-82-	SALSDL...	82			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-82-acetylation	SALSDL...	82	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-83-	ALSDLH...	83			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-99-unknown	FKLLSH...	99		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC16756
P69905-107-unknown	LVTLAA...	107		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC4610
P69905-110-unknown	LAAHLP...	110		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC4611
P69905-111-unknown	AAHLPA...	111		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC4612
P69905-128-	KFLASV...	128			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-128-acetylation	KFLASV...	128	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-129-acetylation	FLASVS...	129	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-130-unknown	LASVST...	130		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC4613
P69905-130-	LASVST...	130			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-130-acetylation	LASVST...	130	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-131-	ASVSTV...	131			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-131-acetylation	ASVSTV...	131	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-132-	SVSTVL...	132			N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-132-acetylation	SVSTVL...	132	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P69905-135-unknown	TVLTSK...	135		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC4614

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...ADKTNV	10	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16754
...GAEALE	30	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4607
...LERMFL	34	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4608
...MFLSFP	37	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4609
...DALTNA	69	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16755
...VDPVNF	98	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC16756
...LSHCLL	106	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4610
...CLLVTL	109	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4611
...LLVTLA	110	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4612
...SLDKFL	129	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4613
...LASVST	134	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4614
...LTSKYR	141	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8316
...LTSKYR	141	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8337
...TSKYR	142	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
LGMN_HUMAN	10	KTNV.\|.VKAA	inferred from experiment	unknown	MEROPS	Kopacek P	Sojka D et al.:IrAE: an asparaginyl endopeptid... (C13.004)	17336985,
CATD_HUMAN	30	EALE.\|.ERMF	inferred from experiment	unknown	MEROPS	McManus DP	Brindley PJ et al.:Proteolysis of human hemoglobin... (A01.009)	11166391,
CATD_HUMAN	34	RMFL.\|.LSFP	inferred from experiment	unknown	MEROPS	McManus DP	Brindley PJ et al.:Proteolysis of human hemoglobin... (A01.009)	11166391,
CATD_HUMAN	37	LSFP.\|.PTTK	inferred from experiment	unknown	MEROPS	McManus DP	Brindley PJ et al.:Proteolysis of human hemoglobin... (A01.009)	11166391,
LGMN_HUMAN	69	LTNA.\|.AVAH	inferred from experiment	unknown	MEROPS	Kopacek P	Sojka D et al.:IrAE: an asparaginyl endopeptid... (C13.004)	17336985,
LGMN_HUMAN	98	PVNF.\|.FKLL	inferred from experiment	unknown	MEROPS	Kopacek P	Sojka D et al.:IrAE: an asparaginyl endopeptid... (C13.004)	17336985,
CATD_HUMAN	106	HCLL.\|.LVTL	inferred from experiment	unknown	MEROPS	McManus DP	Brindley PJ et al.:Proteolysis of human hemoglobin... (A01.009)	11166391,
CATD_HUMAN	109	LVTL.\|.LAAH	inferred from experiment	unknown	MEROPS	McManus DP	Brindley PJ et al.:Proteolysis of human hemoglobin... (A01.009)	11166391,
CATD_HUMAN	110	VTLA.\|.AAHL	inferred from experiment	unknown	MEROPS	McManus DP	Brindley PJ et al.:Proteolysis of human hemoglobin... (A01.009)	11166391,
CATD_HUMAN	129	DKFL.\|.LASV	inferred from experiment	unknown	MEROPS	McManus DP	Brindley PJ et al.:Proteolysis of human hemoglobin... (A01.009)	11166391,
CATD_HUMAN	134	SVST.\|.TVLT	inferred from experiment	unknown	MEROPS	McManus DP	Brindley PJ et al.:Proteolysis of human hemoglobin... (A01.009)	11166391,
CBPM_HUMAN	141	SKYR.\|.R	inferred from experiment	unknown	MEROPS	Erdos EG	Michel B et al.:Removal of Arg141 from the alph... (M14.006)	8635221,
CBPN_HUMAN	141	SKYR.\|.R	inferred from experiment	unknown	MEROPS	Erdos EG	Michel B et al.:Removal of Arg141 from the alph... (M14.006)	8635221,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)