P14174: Macrophage migration inhibitory factor

General Information

Protein names	- Macrophage migration inhibitory factor - MIF - 5.3.2.1 - Glycosylation-inhibiting factor - GIF - L-dopachrome isomerase - L-dopachrome tautomerase - 5.3.3.12 - Phenylpyruvate tautomerase
Gene names	MIF
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P14174

15 N-termini

9 C-termini

12 Cleavages

0 Substrates

Sequence

10 20 30 40 50 60
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC
70 80 90 100 110
SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

Isoforms

Sequence View

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Protein Neighborhood

Domains & Features

15 N-termini - 9 C-termini - 12 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P14174-1-	MPMFIV...	1		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P14174-1-unknown	MPMFIV...	1		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P14174-1-unknown	MPMFIV...	1		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P14174-2-unknown	PMFIVN...	2	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P14174-2-unknown	PMFIVN...	2	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18728
P14174-2-unknown	PMFIVN...	2		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P14174-2-unknown	PMFIVN...	2		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P14174-2-unmodified	PMFIVN...	2		other		CNRS/ISV	Large_scale_NTA_Human
P14174-3-	MFIVNT...	3		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P14174-3-	MFIVNT...	3		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P14174-3-	MFIVNT...	3		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P14174-3-	MFIVNT...	3		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P14174-3-	MFIVNT...	3		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P14174-3-	MFIVNT...	3		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P14174-4-	FIVNTN...	4		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P14174-4-	FIVNTN...	4		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P14174-4-	FIVNTN...	4		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P14174-5-	IVNTNV...	5		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P14174-29-unknown	QATGKP...	29	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7318
P14174-30-unknown	ATGKPP...	30	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3643
P14174-30-unknown	ATGKPP...	30	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7319
P14174-32-unknown	GKPPQY...	32	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7320
P14174-33-unknown	KPPQYI...	33	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7321
P14174-49-unknown	AFGGSS...	49	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3644
P14174-50-unknown	FGGSSE...	50	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3645
P14174-50-unknown	FGGSSE...	50	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7322
P14174-52-unknown	GSSEPC...	52	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5599
P14174-64-unknown	SIGKIG...	64	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7323
P14174-64-unknown	SIGKIG...	64	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5600

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...TQQLAQ	28	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7318
...QQLAQA	29	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3643
...QQLAQA	29	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7319
...LAQATG	31	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7320
...AQATGK	32	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7321
...PDQLMA	48	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3644
...DQLMAF	49	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3645
...DQLMAF	49	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7322
...LMAFGG	51	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5599
...LCSLHS	63	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7323
...LCSLHS	63	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5600
...NSTFA	115	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
MEP1B_HUMAN	1	.\|.PMFI	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATS_HUMAN	28	QLAQ.\|.QATG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	29	LAQA.\|.ATGK	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	29	LAQA.\|.ATGK	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	31	QATG.\|.GKPP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	32	ATGK.\|.KPPQ	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	48	QLMA.\|.AFGG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	49	LMAF.\|.FGGS	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	49	LMAF.\|.FGGS	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	51	AFGG.\|.GSSE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	63	SLHS.\|.SIGK	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	63	SLHS.\|.SIGK	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)