P26368: Splicing factor U2AF 65 kDa subunit

General Information

Protein names	- Splicing factor U2AF 65 kDa subunit - U2 auxiliary factor 65 kDa subunit - hU2AF(65) - hU2AF65 - U2 snRNP auxiliary factor large subunit
Gene names	U2AF2
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P26368

17 N-termini

10 C-termini

12 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MSDFDEFERQ LNENKQERDK ENRHRKRSHS RSRSRDRKRR SRSRDRRNRD QRSASRDRRR
        70         80         90        100        110        120
RSKPLTRGAK EEHGGLIRSP RHEKKKKVRK YWDVPPPGFE HITPMQYKAM QAAGQIPATA
       130        140        150        160        170        180
LLPTMTPDGL AVTPTPVPVV GSQMTRQARR LYVGNIPFGI TEEAMMDFFN AQMRLGGLTQ
       190        200        210        220        230        240
APGNPVLAVQ INQDKNFAFL EFRSVDETTQ AMAFDGIIFQ GQSLKIRRPH DYQPLPGMSE
       250        260        270        280        290        300
NPSVYVPGVV STVVPDSAHK LFIGGLPNYL NDDQVKELLT SFGPLKAFNL VKDSATGLSK
       310        320        330        340        350        360
GYAFCEYVDI NVTDQAIAGL NGMQLGDKKL LVQRASVGAK NATLVSPPST INQTPVTLQV
       370        380        390        400        410        420
PGLMSSQVQM GGHPTEVLCL MNMVLPEELL DDEEYEEIVE DVRDECSKYG LVKSIEIPRP
       430        440        450        460        470
VDGVEVPGCG KIFVEFTSVF DCQKAMQGLT GRKFANRVVV TKYCDPDSYH RRDFW

Isoforms

- Isoform 2 of Splicing factor U2AF 65 kDa subunit

Sequence View

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Protein Neighborhood

Domains & Features

17 N-termini - 10 C-termini - 12 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P26368-2-unknown	MSDFDE...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P26368-1-unknown	MSDFDE...	1		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt93516
P26368-2-Acetylation	SDFDEF...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P26368-2-Acetylation	SDFDEF...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P26368-2-Acetylation	SDFDEF...	2	acetylation-		other		CNRS/ISV	Large_scale_NTA_Human
P26368-2-Acetylation	SDFDEF...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P26368-2-Acetylation	SDFDEF...	2	acetylation-	inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt110275
P26368-10-unknown	QLNENK...	10		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10041
P26368-10-unknown	QLNENK...	10		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt194862
P26368-68-	GAKEEH...	68			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P26368-68-unknown	GAKEEH...	68		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10042
P26368-68-unknown	GAKEEH...	68		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt140489
P26368-100-unknown	EHITPM...	100		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18978
P26368-100-unknown	EHITPM...	100		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt142388
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_CD95	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	RMPI_untreated	23264352
P26368-129-	GLAVTP...	129			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P26368-129-unknown	GLAVTP...	129		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2377
P26368-129-unknown	GLAVTP...	129		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11365
P26368-129-unknown	GLAVTP...	129		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1205
P26368-129-unknown	GLAVTP...	129			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P26368-129-unknown	GLAVTP...	129		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt143846
P26368-131-unknown	AVTPTP...	131		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11367
P26368-131-unknown	AVTPTP...	131		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC14047
P26368-131-unknown	AVTPTP...	131			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P26368-131-unknown	AVTPTP...	131		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt143934
P26368-131-	AVTPTP...	131			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P26368-131-	AVTPTP...	131			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P26368-131-	AVTPTP...	131			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P26368-131-	AVTPTP...	131			Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
P26368-131-	AVTPTP...	131			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P26368-131-	AVTPTP...	131			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P26368-131-	AVTPTP...	131			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P26368-131-	AVTPTP...	131			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P26368-131-	AVTPTP...	131			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P26368-132-	VTPTPV...	132			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P26368-132-unknown	VTPTPV...	132		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt165416
P26368-133-unknown	TPTPVP...	133		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11369
P26368-133-unknown	TPTPVP...	133		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt144003
P26368-265-unknown	GLPNYL...	265		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5726
P26368-265-unknown	GLPNYL...	265		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt148978
P26368-417-unknown	IPRPVD...	417		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5728
P26368-417-unknown	IPRPVD...	417		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt152202
P26368-468-unknown	SYHRRD...	468		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11371
P26368-468-unknown	SYHRRD...	468		inferred from isoform by sequence similarity	unknown	TopFIND		inferred from TNt152965

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...DEFERQ	9	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10041
...KPLTRG	67	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10042
...KPLTRG	67	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt123616
...PPPGFE	99	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18978
...PPPGFE	99	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt125580
...TMTPDG	128	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2377
...TMTPDG	128	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11365
...TMTPDG	128	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC1205
...TMTPDG	128	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt127090
...TPDGLA	130	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11367
...TPDGLA	130	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC14047
...TPDGLA	130	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt127177
...DGLAVT	132	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11369
...DGLAVT	132	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt127247
...KLFIGG	264	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5726
...KLFIGG	264	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt132359
...VKSIEI	416	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5728
...VKSIEI	416	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt135612
...YCDPDS	467	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11371
...YCDPDS	467	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt136391
...RRDFW	475	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
...RRDFW	475	inferred from isoform by sequence similarity	unknown	TopFIND	inferred from TCt89134

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAA_HUMAN	9	FERQ.\|.QLNE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:The substrate specificity profi... (S01.135)	20536382,
GRAA_HUMAN	67	LTRG.\|.GAKE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:The substrate specificity profi... (S01.135)	20536382,
MEP1B_HUMAN	99	PGFE.\|.EHIT	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CASP4_HUMAN	128	TPDG.\|.GLAV	inferred from experiment	unknown	MEROPS	Wells JA	Agard NJ et al.:Inflammatory stimuli regulate c... (C14.001)	20173201,
GRAB_HUMAN	128	TPDG.\|.GLAV	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
CASP1_HUMAN	128	TPDG.\|.GLAV	inferred from experiment	unknown	MEROPS	Wells JA	Agard NJ et al.:Inflammatory stimuli regulate c... (C14.001)	20173201,
GRAB_HUMAN	130	DGLA.\|.AVTP	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
GRAM_HUMAN	130	DGLA.\|.AVTP	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,
GRAB_HUMAN	132	LAVT.\|.TPTP	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
CATL1_HUMAN	264	FIGG.\|.GLPN	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	416	SIEI.\|.IPRP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
GRAB_HUMAN	467	DPDS.\|.SYHR	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)