P34932: Heat shock 70 kDa protein 4

General Information

Protein names	- Heat shock 70 kDa protein 4 - HSP70RY - Heat shock 70-related protein APG-2
Gene names	HSPA4
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P34932

19 N-termini

10 C-termini

10 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACIS FGPKNRSIGA AAKSQVISNA
        70         80         90        100        110        120
KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG IKVTYMEEER NFTTEQVTAM
       130        140        150        160        170        180
LLSKLKETAE SVLKKPVVDC VVSVPCFYTD AERRSVMDAT QIAGLNCLRL MNETTAVALA
       190        200        210        220        230        240
YGIYKQDLPA LEEKPRNVVF VDMGHSAYQV SVCAFNRGKL KVLATAFDTT LGGRKFDEVL
       250        260        270        280        290        300
VNHFCEEFGK KYKLDIKSKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDVDVSGT
       310        320        330        340        350        360
MNRGKFLEMC NDLLARVEPP LRSVLEQTKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK
       370        380        390        400        410        420
ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGSSDCEVF
       430        440        450        460        470        480
SKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV
       490        500        510        520        530        540
KVRVNVHGIF SVSSASLVEV HKSEENEEPM ETDQNAKEEE KMQVDQEEPH VEEQQQQTPA
       550        560        570        580        590        600
ENKAESEEME TSQAGSKDKK MDQPPQAKKA KVKTSTVDLP IENQLLWQID REMLNLYIEN
       610        620        630        640        650        660
EGKMIMQDKL EKERNDAKNA VEEYVYEMRD KLSGEYEKFV SEDDRNSFTL KLEDTENWLY
       670        680        690        700        710        720
EDGEDQPKQV YVDKLAELKN LGQPIKIRFQ ESEERPKLFE ELGKQIQQYM KIISSFKNKE
       730        740        750        760        770        780
DQYDHLDAAD MTKVEKSTNE AMEWMNNKLN LQNKQSLTMD PVVKSKEIEA KIKELTSTCS
       790        800        810        820        830        840
PIISKPKPKV EPPKEEQKNA EQNGPVDGQG DNPGPQAAEQ GTDTAVPSDS DKKLPEMDID

Isoforms

- Isoform 2 of Heat shock 70 kDa protein 4

Sequence View

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Protein Neighborhood

Domains & Features

19 N-termini - 10 C-termini - 10 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P34932-1-unknown	MSVVGI...	1	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P34932-74-	AFSDPF...	74		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P34932-74-	AFSDPF...	74		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P34932-90-unknown	DIVQLP...	90	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19060
P34932-94-unknown	LPTGLT...	94	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5810
P34932-180-	AYGIYK...	180		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P34932-182-	GIYKQD...	182		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P34932-225-unknown	TAFDTT...	225	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7445
P34932-309-unknown	MCNDLL...	309	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7446
P34932-335-unknown	IYAVEI...	335	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19061
P34932-343-unknown	GATRIP...	343	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5811
P34932-363-	STTLNA...	363		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P34932-364-	TTLNAD...	364		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P34932-365-	TLNADE...	365		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P34932-463-	SVQKVT...	463		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P34932-500-unknown	VHKSEE...	500	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5812
P34932-533-unknown	EQQQQT...	533	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7447
P34932-596-unknown	LYIENE...	596	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC19062
P34932-596-unknown	LYIENE...	596	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17603
P34932-620-	AVEEYV...	620		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P34932-620-	AVEEYV...	620		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P34932-620-	AVEEYV...	620		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P34932-620-	AVEEYV...	620		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P34932-728-unknown	AADMTK...	728		unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...SNLAYD	89	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19060
...YDIVQL	93	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5810
...LKVLAT	224	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7445
...GKFLEM	308	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7446
...LKKEDI	334	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19061
...VEIVGG	342	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5811
...ASLVEV	499	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5812
...EPHVEE	532	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7447
...REMLNL	595	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19062
...REMLNL	595	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17603
...EMDID	840	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
MEP1B_HUMAN	89	LAYD.\|.DIVQ	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATL1_HUMAN	93	IVQL.\|.LPTG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	224	VLAT.\|.TAFD	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	308	FLEM.\|.MCND	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	334	KEDI.\|.IYAV	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATL1_HUMAN	342	IVGG.\|.GATR	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	499	LVEV.\|.VHKS	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	532	HVEE.\|.EQQQ	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	595	MLNL.\|.LYIE	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1A_HUMAN	595	MLNL.\|.LYIE	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)