P68134: Actin, alpha skeletal muscle

General Information

Protein names	- Actin, alpha skeletal muscle - Alpha-actin-1 - Actin, alpha skeletal muscle, intermediate form
Gene names	Acta1
Organism	Mus musculus
Protease Family
Protease ID
Chromosome location
UniProt ID	P68134

33 N-termini

15 C-termini

20 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MCDEDETTAL VCDNGSGLVK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA
        70         80         90        100        110        120
QSKRGILTLK YPIEHGIITN WDDMEKIWHH TFYNELRVAP EEHPTLLTEA PLNPKANREK
       130        140        150        160        170        180
MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VTHNVPIYEG YALPHAIMRL
       190        200        210        220        230        240
DLAGRDLTDY LMKILTERGY SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK
       250        260        270        280        290        300
SYELPDGQVI TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV
       310        320        330        340        350        360
MSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS LSTFQQMWIT
       370
KQEYDEAGPS IVHRKCF

Isoforms

Sequence View

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Protein Neighborhood

Domains & Features

33 N-termini - 15 C-termini - 20 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P68134-3-Acetylation	DEDETT...	3	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P68134-3-Acetylation	DEDETT...	3	acetylation-	inferred from similarity	unknown	UniProtKB		inferred from uniprot (by similarity)
P68134-9-unknown	ALVCDN...	9		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC33767
P68134-20-unmodified	KAGFAG...	20			N-TAILS		Overall CM.	Prudova A.: Multiplex N-terminome analysis of MMP-2 and MMP-9...	20305284
P68134-21-unmodified	AGFAGD...	21			unknown			Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from...	20208520
P68134-23-unmodified	FAGDDA...	23			unknown			Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from...	20208520
P68134-42-unmodified	HQGVMV...	42			N-TAILS		Overall CM.	Prudova A.: Multiplex N-terminome analysis of MMP-2 and MMP-9...	20305284
P68134-44-unmodified	GVMVGM...	44			N-TAILS		Overall CM.	Prudova A.: Multiplex N-terminome analysis of MMP-2 and MMP-9...	20305284
P68134-46-unknown	MVGMGQ...	46		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC33768
P68134-47-unknown	VGMGQK...	47		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC30292
P68134-49-unmodified	MGQKDS...	49			unknown			Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from...	20208520
P68134-50-unmodified	GQKDSY...	50			unknown			Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from...	20208520
P68134-54-unmodified	SYVGDE...	54			unknown			Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from...	20208520
P68134-56-unknown	VGDEAQ...	56		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC30293
P68134-106-unmodified	LLTEAP...	106			unknown			Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from...	20208520
P68134-106-unmodified	LLTEAP...	106			N-TAILS		Overall CM.	Prudova A.: Multiplex N-terminome analysis of MMP-2 and MMP-9...	20305284
P68134-107-unmodified	LTEAPL...	107			unknown			Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from...	20208520
P68134-107-unknown	LTEAPL...	107		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC30294
P68134-107-unknown	LTEAPL...	107		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC29057
P68134-107-unknown	LTEAPL...	107		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC33769
P68134-139-unknown	QAVLSL...	139		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC30295
P68134-142-unknown	LSLYAS...	142		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC30296
P68134-188-unmodified	TDYLMK...	188			unknown			Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from...	20208520
P68134-192-unknown	MKILTE...	192		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC30297
P68134-230-unknown	ATAASS...	230		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC29058
P68134-231-unmodified	TAASSS...	231			N-TAILS		Overall CM.	Kleifeldt O. et al.: Isotopic labeling of terminal amines in complex samples identifies...	20208520
P68134-231-unmodified	TAASSS...	231			N-TAILS		Overall CM.	Prudova A.: Multiplex N-terminome analysis of MMP-2 and MMP-9...	20305284
P68134-234-unmodified	SSSSLE...	234			unknown			Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from...	20208520
P68134-234-unknown	SSSSLE...	234		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC33770
P68134-235-unknown	SSSLEK...	235		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC33771
P68134-237-unmodified	SLEKSY...	237			N-TAILS		Overall CM.	Prudova A.: Multiplex N-terminome analysis of MMP-2 and MMP-9...	20305284
P68134-241-unmodified	SYELPD...	241			N-TAILS		Overall CM.	Kleifeldt O. et al.: Isotopic labeling of terminal amines in complex samples identifies...	20208520
P68134-241-unmodified	SYELPD...	241			unknown			Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from...	20208520
P68134-247-unknown	GQVITI...	247		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC28490
P68134-247-unknown	GQVITI...	247		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC32383
P68134-247-unknown	GQVITI...	247		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC28569
P68134-247-unknown	GQVITI...	247		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC28369
P68134-247-unmodified	GQVITI...	247			unknown			Kleifeldt O. et al.: N-terminome analysis of bronchoalveolar lavage fluids collected from...	20208520
P68134-302-unknown	SGGTTM...	302		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC30298
P68134-302-unknown	SGGTTM...	302		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC29059
P68134-318-unmodified	EITALA...	318			N-TAILS		Overall CM.	Kleifeldt O. et al.: Isotopic labeling of terminal amines in complex samples identifies...	20208520
P68134-318-unmodified	EITALA...	318			N-TAILS		Overall CM.	Prudova A.: Multiplex N-terminome analysis of MMP-2 and MMP-9...	20305284
P68134-327-unknown	MKIKII...	327		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC33772
P68134-329-unmodified	IKIIAP...	329			N-TAILS		Overall CM.	Kleifeldt O. et al.: Isotopic labeling of terminal amines in complex samples identifies...	20208520

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...EDETTA	8	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC33767
...RHQGVM	45	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC33768
...HQGVMV	46	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC30292
...QKDSYV	55	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC30293
...EHPTLL	106	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC30294
...EHPTLL	106	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC29057
...EHPTLL	106	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC33769
...MYVAIQ	138	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC30295
...AIQAVL	141	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC30296
...LTDYLM	191	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC30297
...FENEMA	229	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC29058
...MATAAS	233	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC33770
...ATAASS	234	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC33771
...YELPDG	246	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC28490
...YELPDG	246	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC32383
...YELPDG	246	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC28569
...YELPDG	246	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC28369
...ANNVMS	301	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC30298
...ANNVMS	301	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC29059
...LAPSTM	326	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC33772
...HRKCF	377	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
MMP2_MOUSE	8	ETTA.\|.ALVC	inferred from experiment	unknown	MEROPS	Overall CM	Prudova A et al.:Multiplex N-terminome analysis ... (M10.004)	20305284,
MMP2_MOUSE	45	QGVM.\|.MVGM	inferred from experiment	unknown	MEROPS	Overall CM	Prudova A et al.:Multiplex N-terminome analysis ... (M10.004)	20305284,
CATE_MOUSE	46	GVMV.\|.VGMG	inferred from experiment	unknown	MEROPS		Merops CATE_MOUSE -> ACTS_MOUSE @46
CATE_MOUSE	55	DSYV.\|.VGDE	inferred from experiment	unknown	MEROPS		Merops CATE_MOUSE -> ACTS_MOUSE @55
CATE_MOUSE	106	PTLL.\|.LTEA	inferred from experiment	unknown	MEROPS		Merops CATE_MOUSE -> ACTS_MOUSE @106
CATD_MOUSE	106	PTLL.\|.LTEA	inferred from experiment	unknown	MEROPS	Zornig M	Melzer IM et al.:The Apaf-1-binding protein Aven... (A01.009)	22388353,
MMP2_MOUSE	106	PTLL.\|.LTEA	inferred from experiment	unknown	MEROPS	Overall CM	Prudova A et al.:Multiplex N-terminome analysis ... (M10.004)	20305284,
CATE_MOUSE	138	VAIQ.\|.QAVL	inferred from experiment	unknown	MEROPS		Merops CATE_MOUSE -> ACTS_MOUSE @138
CATE_MOUSE	141	QAVL.\|.LSLY	inferred from experiment	unknown	MEROPS		Merops CATE_MOUSE -> ACTS_MOUSE @141
CATE_MOUSE	191	DYLM.\|.MKIL	inferred from experiment	unknown	MEROPS		Merops CATE_MOUSE -> ACTS_MOUSE @191
CATD_MOUSE	229	NEMA.\|.ATAA	inferred from experiment	unknown	MEROPS	Zornig M	Melzer IM et al.:The Apaf-1-binding protein Aven... (A01.009)	22388353,
MMP2_MOUSE	233	TAAS.\|.SSSS	inferred from experiment	unknown	MEROPS		Merops MMP2_MOUSE -> ACTS_MOUSE @233
MMP2_MOUSE	234	AASS.\|.SSSL	inferred from experiment	unknown	MEROPS	Overall CM	Prudova A et al.:Multiplex N-terminome analysis ... (M10.004)	20305284,
CASP3_MOUSE	246	LPDG.\|.GQVI	inferred from experiment	unknown	MEROPS	Vandenabeele P	Demon D et al.:Proteome-wide substrate analysi... (C14.003)	19759058,
GRAB_MOUSE	246	LPDG.\|.GQVI	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Analysis of protein processing ... (S01.010)	18836177,
CASP7_MOUSE	246	LPDG.\|.GQVI	inferred from experiment	unknown	MEROPS	Vandenabeele P	Demon D et al.:Proteome-wide substrate analysi... (C14.003)	19759058,
CASP1_MOUSE	246	LPDG.\|.GQVI	inferred from experiment	unknown	MEROPS	Nunez G	Lamkanfi M et al.:Targeted peptidecentric proteom... (C14.001)	18667412,
CATE_MOUSE	301	NVMS.\|.SGGT	inferred from experiment	unknown	MEROPS		Merops CATE_MOUSE -> ACTS_MOUSE @301
CATD_MOUSE	301	NVMS.\|.SGGT	inferred from experiment	unknown	MEROPS	Zornig M	Melzer IM et al.:The Apaf-1-binding protein Aven... (A01.009)	22388353,
MMP2_MOUSE	326	PSTM.\|.MKIK	inferred from experiment	unknown	MEROPS	Overall CM	Prudova A et al.:Multiplex N-terminome analysis ... (M10.004)	20305284,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)