P00558: Phosphoglycerate kinase 1

General Information

Protein names	- Phosphoglycerate kinase 1 - 2.7.2.3 - Cell migration-inducing gene 10 protein - Primer recognition protein 2 - PRP 2
Gene names	PGK1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P00558

34 N-termini

20 C-termini

25 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL
        70         80         90        100        110        120
MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE
       130        140        150        160        170        180
NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN
       190        200        210        220        230        240
LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM
       250        260        270        280        290        300
AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ
       310        320        330        340        350        360
ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV
       370        380        390        400        410
KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI

Isoforms

- Isoform 2 of Phosphoglycerate kinase 1

Sequence View

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Protein Neighborhood

Domains & Features

34 N-termini - 20 C-termini - 25 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P00558-2-unknown	MSLSNK...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P00558-2-Acetylation	SLSNKL...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P00558-2-Acetylation	SLSNKL...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P00558-2-Acetylation	SLSNKL...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P00558-2-Acetylation	SLSNKL...	2	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P00558-2-	SLSNKL...	2			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P00558-11-unknown	KLDVKG...	11		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11039
P00558-23-acetylation	VDFNVP...	23	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P00558-29-unknown	MKNNQI...	29		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000470986
P00558-29-unknown	MKNNQI...	29		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000444708
P00558-65-unknown	GRPDGV...	65		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7102
P00558-66-unknown	RPDGVP...	66		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7103
P00558-66-unknown	RPDGVP...	66		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5317
P00558-69-	GVPMPD...	69			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P00558-69-	GVPMPD...	69			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P00558-69-	GVPMPD...	69			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P00558-77-	SLEPVA...	77			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P00558-77-	SLEPVA...	77			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P00558-104-unknown	VEKACA...	104		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11040
P00558-152-acetylation	ASLSKL...	152	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P00558-160-	VYVNDA...	160			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P00558-160-	VYVNDA...	160			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P00558-176-unknown	MVGVNL...	176		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3319
P00558-197-	FAKALE...	197			Subtiligase Based Positive Selection		Wells	apopotic_Jurkat_FasL	23264352
P00558-200-	ALESPE...	200			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P00558-200-	ALESPE...	200			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P00558-200-	ALESPE...	200			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P00558-200-	ALESPE...	200			Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P00558-200-	ALESPE...	200			Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P00558-200-	ALESPE...	200			Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P00558-200-	ALESPE...	200			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P00558-202-unknown	ESPERP...	202		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18346
P00558-203-unknown	SPERPF...	203		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18347
P00558-253-unknown	IGTSLF...	253		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7104
P00558-255-unknown	TSLFDE...	255		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7105
P00558-275-acetylation	KNGVKI...	275	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P00558-277-	GVKITL...	277			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P00558-286-	FVTADK...	286			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P00558-286-	FVTADK...	286			Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P00558-286-unknown	FVTADK...	286		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3320
P00558-289-unknown	ADKFDE...	289		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7106
P00558-289-unknown	ADKFDE...	289		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5318
P00558-290-unknown	DKFDEN...	290		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7107
P00558-311-unknown	WMGLDC...	311		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3321
P00558-313-unknown	GLDCGP...	313		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7108
P00558-313-unknown	GLDCGP...	313		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5319
P00558-313-unknown	GLDCGP...	313		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3322
P00558-316-unknown	CGPESS...	316		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11041
P00558-354-	ALMDEV...	354			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P00558-362-unknown	ATSRGC...	362		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5320
P00558-362-unknown	ATSRGC...	362		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P00558-362-unknown	ATSRGC...	362		inferred from similarity	unknown	UniProtKB		inferred from uniprot (by similarity)
P00558-370-unknown	IIGGGD...	370		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7109
P00558-370-unknown	IIGGGD...	370		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5321
P00558-370-unknown	IIGGGD...	370		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3323
P00558-373-unknown	GGDTAT...	373		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7110
P00558-396-unknown	GGASLE...	396		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5322

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...KLTLDK	10	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11039
...LMSHLG	64	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7102
...MSHLGR	65	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7103
...MSHLGR	65	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5317
...CVGPEV	103	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11040
...RAHSSM	175	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3319
...FAKALE	201	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18346
...AKALES	202	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18347
...LNNMEI	252	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7104
...NMEIGT	254	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7105
...TLPVDF	285	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3320
...VDFVTA	288	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7106
...VDFVTA	288	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5318
...DFVTAD	289	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7107
...GIPAGW	310	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3321
...PAGWMG	312	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7108
...PAGWMG	312	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5319
...PAGWMG	312	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3322
...WMGLDC	315	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11041
...DEVVKA	361	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5320
...RGCITI	369	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7109
...RGCITI	369	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5321
...RGCITI	369	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3323
...ITIIGG	372	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7110
...HVSTGG	395	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5322
...ALSNI	417	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
GRAB_HUMAN	10	TLDK.\|.KLDV	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,
CATS_HUMAN	64	SHLG.\|.GRPD	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	65	HLGR.\|.RPDG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	65	HLGR.\|.RPDG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
GRAB_HUMAN	103	GPEV.\|.VEKA	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,
CATB_HUMAN	175	HSSM.\|.MVGV	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	201	KALE.\|.ESPE	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1B_HUMAN	202	ALES.\|.SPER	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATS_HUMAN	252	NMEI.\|.IGTS	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	254	EIGT.\|.TSLF	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	285	PVDF.\|.FVTA	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	288	FVTA.\|.ADKF	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	288	FVTA.\|.ADKF	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	289	VTAD.\|.DKFD	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	310	PAGW.\|.WMGL	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	312	GWMG.\|.GLDC	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	312	GWMG.\|.GLDC	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	312	GWMG.\|.GLDC	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
GRAB_HUMAN	315	GLDC.\|.CGPE	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,
CATL1_HUMAN	361	VVKA.\|.ATSR	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	369	CITI.\|.IIGG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	369	CITI.\|.IIGG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATB_HUMAN	369	CITI.\|.IIGG	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATS_HUMAN	372	IIGG.\|.GGDT	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CATL1_HUMAN	395	STGG.\|.GGAS	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)