P01210: Proenkephalin-A

General Information

Protein names	- Proenkephalin-A - Synenkephalin - Met-enkephalin - Opioid growth factor - OGF - PENK(114-133) - PENK(143-183) - Met-enkephalin-Arg-Gly-Leu - Leu-enkephalin - PENK(237-258) - Met-enkephalin-Arg-Phe
Gene names	PENK
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P01210

21 N-termini

22 C-termini

41 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MARFLTLCTW LLLLGPGLLA TVRAECSQDC ATCSYRLVRP ADINFLACVM ECEGKLPSLK
        70         80         90        100        110        120
IWETCKELLQ LSKPELPQDG TSTLRENSKP EESHLLAKRY GGFMKRYGGF MKKMDELYPM
       130        140        150        160        170        180
EPEEEANGSE ILAKRYGGFM KKDAEEDDSL ANSSDLLKEL LETGDNRERS HHQDGSDNEE
       190        200        210        220        230        240
EVSKRYGGFM RGLKRSPQLE DEAKELQKRY GGFMRRVGRP EWWMDYQKRY GGFLKRFAEA
       250        260
LPSDEEGESY SKEVPEMEKR YGGFMRF

Isoforms

Sequence View

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Protein Neighborhood

Domains & Features

21 N-termini - 22 C-termini - 41 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Evidence name
P01210-4-unknown	FLTLCT...	4	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000430268
P01210-25-unknown	ECSQDC...	25	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
P01210-98-unknown	KRYGGF...	98	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6964
P01210-103-unknown	FMKRYG...	103	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26697
P01210-103-unknown	FMKRYG...	103	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19900
P01210-104-unknown	MKRYGG...	104	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8220
P01210-104-unknown	MKRYGG...	104	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8257
P01210-105-unknown	KRYGGF...	105	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6965
P01210-134-unknown	KRYGGF...	134	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6966
P01210-134-unknown	KRYGGF...	134	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot
P01210-134-unknown	KRYGGF...	134	inferred from similarity	unknown	UniProtKB	inferred from uniprot (by similarity)
P01210-143-unknown	DAEEDD...	143	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26965
P01210-143-unknown	DAEEDD...	143	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27261
P01210-143-unknown	DAEEDD...	143	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26505
P01210-143-unknown	DAEEDD...	143	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26548
P01210-143-unknown	DAEEDD...	143	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26820
P01210-143-unknown	DAEEDD...	143	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9686
P01210-143-unknown	DAEEDD...	143	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27112
P01210-186-unknown	YGGFMR...	186	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26506
P01210-186-unknown	YGGFMR...	186	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26549
P01210-192-unknown	GLKRSP...	192	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3332
P01210-196-unknown	SPQLED...	196	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26966
P01210-196-unknown	SPQLED...	196	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27262
P01210-196-unknown	SPQLED...	196	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26507
P01210-196-unknown	SPQLED...	196	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26550
P01210-196-unknown	SPQLED...	196	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26821
P01210-196-unknown	SPQLED...	196	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9687
P01210-196-unknown	SPQLED...	196	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27113
P01210-208-unknown	KRYGGF...	208	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6967
P01210-214-unknown	MRRVGR...	214	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8258
P01210-227-unknown	QKRYGG...	227	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8259
P01210-230-unknown	YGGFLK...	230	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26508
P01210-230-unknown	YGGFLK...	230	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26551
P01210-233-unknown	FLKRFA...	233	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19901
P01210-234-unknown	LKRFAE...	234	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8260
P01210-235-unknown	KRFAEA...	235	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6968
P01210-236-unknown	RFAEAL...	236	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6969
P01210-237-unknown	FAEALP...	237	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26509
P01210-237-unknown	FAEALP...	237	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26552
P01210-237-unknown	FAEALP...	237	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9688
P01210-261-unknown	YGGFMR...	261	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26510
P01210-261-unknown	YGGFMR...	261	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26553

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...SHLLAK	97	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6964
...KRYGGF	102	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26697
...KRYGGF	102	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19900
...RYGGFM	103	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8220
...RYGGFM	103	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8257
...YGGFMK	104	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6965
...GGFMKR	105	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000428012
...SEILAK	133	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6966
...GFMKKD	142	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26965
...GFMKKD	142	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27261
...GFMKKD	142	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26505
...GFMKKD	142	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26548
...GFMKKD	142	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26820
...GFMKKD	142	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9686
...GFMKKD	142	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27112
...EVSKRY	185	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26506
...EVSKRY	185	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26549
...GGFMRG	191	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3332
...RGLKRS	195	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26966
...RGLKRS	195	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27262
...RGLKRS	195	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26507
...RGLKRS	195	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26550
...RGLKRS	195	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26821
...RGLKRS	195	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9687
...RGLKRS	195	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC27113
...AKELQK	207	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6967
...RYGGFM	213	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8258
...WWMDYQ	226	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8259
...DYQKRY	229	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26508
...DYQKRY	229	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26551
...KRYGGF	232	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC19901
...RYGGFL	233	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8260
...YGGFLK	234	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6968
...GGFLKR	235	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC6969
...GFLKRF	236	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26509
...GFLKRF	236	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26552
...GFLKRF	236	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC9688
...EMEKRY	260	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26510
...EMEKRY	260	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26553
...YGGFMR	265	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3333
...GGFMRF	266	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8221
...GGFMRF	266	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8261

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CATL2_HUMAN	97	LLAK.\|.KRYG	inferred from experiment	unknown	MEROPS	Hook V	Funkelstein L et al.:Human cathepsin V protease part... (C01.009)	22393040,
NEP_HUMAN	102	YGGF.\|.FMKR	inferred from experiment	unknown	MEROPS	Ouimet T	Rose C et al.:Cell-specific activity of nepri... (M13.008)	11964170,
MMEL1_HUMAN	102	YGGF.\|.FMKR	inferred from experiment	unknown	MEROPS	Ouimet T	Rose C et al.:Cell-specific activity of nepri... (M13.008)	11964170,
CBPA4_HUMAN	103	GGFM.\|.MKRY	inferred from experiment	unknown	MEROPS	Fricker LD	Tanco S et al.:Characterization of the substra... (M14.017)	20385563,
CBPA6_HUMAN	103	GGFM.\|.MKRY	inferred from experiment	unknown	MEROPS	Fricker LD	Lyons PJ et al.:Characterization of carboxypept... (M14.018)	18178555,
CATL2_HUMAN	104	GFMK.\|.KRYG	inferred from experiment	unknown	MEROPS	Hook V	Funkelstein L et al.:Human cathepsin V protease part... (C01.009)	22393040,
CATL2_HUMAN	133	ILAK.\|.KRYG	inferred from experiment	unknown	MEROPS	Hook V	Funkelstein L et al.:Human cathepsin V protease part... (C01.009)	22393040,
PCSK5_HUMAN	142	MKKD.\|.DAEE	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
PCSK7_HUMAN	142	MKKD.\|.DAEE	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
NEC1_HUMAN	142	MKKD.\|.DAEE	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
NEC2_HUMAN	142	MKKD.\|.DAEE	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
PCSK4_HUMAN	142	MKKD.\|.DAEE	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
FURIN_HUMAN	142	MKKD.\|.DAEE	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
PCSK6_HUMAN	142	MKKD.\|.DAEE	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
NEC1_HUMAN	185	SKRY.\|.YGGF	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
NEC2_HUMAN	185	SKRY.\|.YGGF	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
CATB_HUMAN	191	FMRG.\|.GLKR	inferred from experiment	unknown	MEROPS	Tang J	Takahashi T et al.:Porcine spleen cathepsin B is a... (C01.060)	3522589,
PCSK5_HUMAN	195	LKRS.\|.SPQL	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
PCSK7_HUMAN	195	LKRS.\|.SPQL	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
NEC1_HUMAN	195	LKRS.\|.SPQL	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
NEC2_HUMAN	195	LKRS.\|.SPQL	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
PCSK4_HUMAN	195	LKRS.\|.SPQL	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
FURIN_HUMAN	195	LKRS.\|.SPQL	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
PCSK6_HUMAN	195	LKRS.\|.SPQL	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
CATL2_HUMAN	207	ELQK.\|.KRYG	inferred from experiment	unknown	MEROPS	Hook V	Funkelstein L et al.:Human cathepsin V protease part... (C01.009)	22393040,
CBPA6_HUMAN	213	GGFM.\|.MRRV	inferred from experiment	unknown	MEROPS	Fricker LD	Lyons PJ et al.:Substrate specificity of human ... (M14.018)	20855895,
CBPA6_HUMAN	226	MDYQ.\|.QKRY	inferred from experiment	unknown	MEROPS	Fricker LD	Lyons PJ et al.:Substrate specificity of human ... (M14.018)	20855895,
NEC1_HUMAN	229	QKRY.\|.YGGF	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
NEC2_HUMAN	229	QKRY.\|.YGGF	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
MMEL1_HUMAN	232	YGGF.\|.FLKR	inferred from experiment	unknown	MEROPS	Ouimet T	Rose C et al.:Cell-specific activity of nepri... (M13.008)	11964170,
CBPA6_HUMAN	233	GGFL.\|.LKRF	inferred from experiment	unknown	MEROPS	Fricker LD	Lyons PJ et al.:Substrate specificity of human ... (M14.018)	20855895,
CATL2_HUMAN	234	GFLK.\|.KRFA	inferred from experiment	unknown	MEROPS	Hook V	Funkelstein L et al.:Human cathepsin V protease part... (C01.009)	22393040,
CATL2_HUMAN	235	FLKR.\|.RFAE	inferred from experiment	unknown	MEROPS	Hook V	Funkelstein L et al.:Human cathepsin V protease part... (C01.009)	22393040,
NEC1_HUMAN	236	LKRF.\|.FAEA	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
NEC2_HUMAN	236	LKRF.\|.FAEA	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
FURIN_HUMAN	236	LKRF.\|.FAEA	inferred from experiment	unknown	MEROPS	Strongin AY	Remacle AG et al.:Substrate cleavage analysis of ... (S08.071)	18505722,
NEC1_HUMAN	260	EKRY.\|.YGGF	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
NEC2_HUMAN	260	EKRY.\|.YGGF	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
CATB_HUMAN	265	GFMR.\|.RF	inferred from experiment	unknown	MEROPS	Tang J	Takahashi T et al.:Porcine spleen cathepsin B is a... (C01.060)	3522589,
CBPA4_HUMAN	266	FMRF.\|.F	inferred from experiment	unknown	MEROPS	Fricker LD	Tanco S et al.:Characterization of the substra... (M14.017)	20385563,
CBPA6_HUMAN	266	FMRF.\|.F	inferred from experiment	unknown	MEROPS	Fricker LD	Lyons PJ et al.:Substrate specificity of human ... (M14.018)	20855895,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)