P01308: Insulin

General Information

Protein names	- Insulin - Insulin B chain - Insulin A chain
Gene names	INS
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P01308

27 N-termini

28 C-termini

45 Cleavages

0 Substrates

Sequence

10 20 30 40 50 60
MALWMRLLPL LALLALWGPD PAAAFVNQHL CGSHLVEALY LVCGERGFFY TPKTRREAED
70 80 90 100 110
LQVGQVELGG GPGAGSLQPL ALEGSLQKRG IVEQCCTSIC SLYQLENYCN

Isoforms

Sequence View

Filter Information:

(REFRESH)

Directness:

Physiological Relevance:

Evidence Codes:

Methodology:

Perturbation of System:

Biological System:

Protease Assignment Confidence:

Evidence Names:

Database:

Lab:

Protein Neighborhood

Domains & Features

27 N-termini - 28 C-termini - 45 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Evidence name
P01308-28-unknown	QHLCGS...	28	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25113
P01308-30-unknown	LCGSHL...	30	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25114
P01308-30-unknown	LCGSHL...	30	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17014
P01308-31-unknown	CGSHLV...	31	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17015
P01308-33-unknown	SHLVEA...	33	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17016
P01308-34-unknown	HLVEAL...	34	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15606
P01308-35-unknown	LVEALY...	35	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25115
P01308-35-unknown	LVEALY...	35	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17017
P01308-35-unknown	LVEALY...	35	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15607
P01308-36-unknown	VEALYL...	36	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4645
P01308-38-unknown	ALYLVC...	38	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4646
P01308-38-unknown	ALYLVC...	38	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15608
P01308-39-unknown	LYLVCG...	39	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25116
P01308-39-unknown	LYLVCG...	39	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4647
P01308-39-unknown	LYLVCG...	39	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17018
P01308-39-unknown	LYLVCG...	39	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15609
P01308-40-unknown	YLVCGE...	40	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4648
P01308-40-unknown	YLVCGE...	40	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17019
P01308-41-unknown	LVCGER...	41	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25117
P01308-41-unknown	LVCGER...	41	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4649
P01308-42-unknown	VCGERG...	42	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4650
P01308-42-unknown	VCGERG...	42	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17020
P01308-45-unknown	ERGFFY...	45	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25118
P01308-45-unknown	ERGFFY...	45	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17021
P01308-46-unknown	RGFFYT...	46	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4651
P01308-48-unknown	FFYTPK...	48	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25119
P01308-49-unknown	FYTPKT...	49	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4652
P01308-49-unknown	FYTPKT...	49	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17022
P01308-49-unknown	FYTPKT...	49	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15610
P01308-50-unknown	YTPKTR...	50	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4653
P01308-50-unknown	YTPKTR...	50	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17023
P01308-50-unknown	YTPKTR...	50	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15611
P01308-55-unknown	RREAED...	55	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8297
P01308-56-unknown	REAEDL...	56	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8298
P01308-57-unknown	EAEDLQ...	57	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26516
P01308-88-unknown	KRGIVE...	88	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8299
P01308-89-unknown	RGIVEQ...	89	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8300
P01308-90-unknown	GIVEQC...	90	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26517
P01308-90-unknown	GIVEQC...	90	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26558
P01308-90-unknown	GIVEQC...	90	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC226
P01308-102-unknown	LYQLEN...	102	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25120
P01308-103-unknown	YQLENY...	103	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15612
P01308-104-unknown	QLENYC...	104	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15613
P01308-105-unknown	LENYCN...	105	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25121

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...AAFVNQ	27	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25113
...FVNQHL	29	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25114
...FVNQHL	29	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17014
...VNQHLC	30	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17015
...QHLCGS	32	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17016
...HLCGSH	33	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15606
...LCGSHL	34	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25115
...LCGSHL	34	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17017
...LCGSHL	34	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15607
...CGSHLV	35	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4645
...SHLVEA	37	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4646
...SHLVEA	37	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15608
...HLVEAL	38	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25116
...HLVEAL	38	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4647
...HLVEAL	38	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17018
...HLVEAL	38	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15609
...LVEALY	39	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4648
...LVEALY	39	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17019
...VEALYL	40	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25117
...VEALYL	40	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4649
...EALYLV	41	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4650
...EALYLV	41	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17020
...YLVCGE	44	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25118
...YLVCGE	44	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17021
...LVCGER	45	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4651
...CGERGF	47	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25119
...GERGFF	48	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4652
...GERGFF	48	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17022
...GERGFF	48	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15610
...ERGFFY	49	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC4653
...ERGFFY	49	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17023
...ERGFFY	49	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15611
...YTPKTR	54	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8297
...TPKTRR	55	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8298
...PKTRRE	56	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26516
...EGSLQK	87	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8299
...GSLQKR	88	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC8300
...SLQKRG	89	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26517
...SLQKRG	89	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC26558
...SLQKRG	89	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC226
...TSICSL	101	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25120
...SICSLY	102	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15612
...ICSLYQ	103	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC15613
...CSLYQL	104	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25121
...LYQLEN	106	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC25122

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
MMP9_HUMAN	27	FVNQ.\|.QHLC	inferred from experiment	unknown	MEROPS	Opdenakker G	Descamps FJ et al.:Gelatinase B is diabetogenic in... (M10.004)	12626433,
MMP9_HUMAN	29	NQHL.\|.LCGS	inferred from experiment	unknown	MEROPS	Opdenakker G	Descamps FJ et al.:Gelatinase B is diabetogenic in... (M10.004)	12626433,
MEP1A_HUMAN	29	NQHL.\|.LCGS	inferred from experiment	unknown	MEROPS	Bond JS	Butler PE et al.:Characterization of meprin, a m... (M12.002)	3105525,
MEP1A_HUMAN	30	QHLC.\|.CGSH	inferred from experiment	unknown	MEROPS	Bond JS	Butler PE et al.:Characterization of meprin, a m... (M12.002)	3105525,
MEP1A_HUMAN	32	LCGS.\|.SHLV	inferred from experiment	unknown	MEROPS	Bond JS	Butler PE et al.:Characterization of meprin, a m... (M12.002)	3105525,
IDE_HUMAN	33	CGSH.\|.HLVE	inferred from experiment	unknown	MEROPS	Tang WJ	Guo Q et al.:Molecular basis for the recogni... (M16.002)	19896952,
MMP9_HUMAN	34	GSHL.\|.LVEA	inferred from experiment	unknown	MEROPS	Opdenakker G	Descamps FJ et al.:Gelatinase B is diabetogenic in... (M10.004)	12626433,
MEP1A_HUMAN	34	GSHL.\|.LVEA	inferred from experiment	unknown	MEROPS	Bond JS	Butler PE et al.:Characterization of meprin, a m... (M12.002)	3105525,
IDE_HUMAN	34	GSHL.\|.LVEA	inferred from experiment	unknown	MEROPS	Tang WJ	Guo Q et al.:Molecular basis for the recogni... (M16.002)	19896952,
CATE_HUMAN	35	SHLV.\|.VEAL	inferred from experiment	unknown	MEROPS	Takahashi K	Athauda SB et al.:Proteolytic activity and cleava... (A01.010)	1959628,
CATE_HUMAN	37	LVEA.\|.ALYL	inferred from experiment	unknown	MEROPS	Takahashi K	Athauda SB et al.:Proteolytic activity and cleava... (A01.010)	1959628,
IDE_HUMAN	37	LVEA.\|.ALYL	inferred from experiment	unknown	MEROPS	Tang WJ	Guo Q et al.:Molecular basis for the recogni... (M16.002)	19896952,
MMP9_HUMAN	38	VEAL.\|.LYLV	inferred from experiment	unknown	MEROPS	Opdenakker G	Descamps FJ et al.:Gelatinase B is diabetogenic in... (M10.004)	12626433,
CATE_HUMAN	38	VEAL.\|.LYLV	inferred from experiment	unknown	MEROPS	Takahashi K	Athauda SB et al.:Proteolytic activity and cleava... (A01.010)	1959628,
MEP1A_HUMAN	38	VEAL.\|.LYLV	inferred from experiment	unknown	MEROPS	Bond JS	Butler PE et al.:Characterization of meprin, a m... (M12.002)	3105525,
IDE_HUMAN	38	VEAL.\|.LYLV	inferred from experiment	unknown	MEROPS	Tang WJ	Guo Q et al.:Molecular basis for the recogni... (M16.002)	19896952,
CATE_HUMAN	39	EALY.\|.YLVC	inferred from experiment	unknown	MEROPS	Takahashi K	Athauda SB et al.:Proteolytic activity and cleava... (A01.010)	1959628,
MEP1A_HUMAN	39	EALY.\|.YLVC	inferred from experiment	unknown	MEROPS	Bond JS	Butler PE et al.:Characterization of meprin, a m... (M12.002)	3105525,
MMP9_HUMAN	40	ALYL.\|.LVCG	inferred from experiment	unknown	MEROPS	Opdenakker G	Descamps FJ et al.:Gelatinase B is diabetogenic in... (M10.004)	12626433,
CATE_HUMAN	40	ALYL.\|.LVCG	inferred from experiment	unknown	MEROPS	Takahashi K	Athauda SB et al.:Proteolytic activity and cleava... (A01.010)	1959628,
CATE_HUMAN	41	LYLV.\|.VCGE	inferred from experiment	unknown	MEROPS	Takahashi K	Athauda SB et al.:Proteolytic activity and cleava... (A01.010)	1959628,
MEP1A_HUMAN	41	LYLV.\|.VCGE	inferred from experiment	unknown	MEROPS	Bond JS	Butler PE et al.:Characterization of meprin, a m... (M12.002)	3105525,
MMP9_HUMAN	44	VCGE.\|.ERGF	inferred from experiment	unknown	MEROPS	Opdenakker G	Descamps FJ et al.:Gelatinase B is diabetogenic in... (M10.004)	12626433,
MEP1A_HUMAN	44	VCGE.\|.ERGF	inferred from experiment	unknown	MEROPS	Bond JS	Butler PE et al.:Characterization of meprin, a m... (M12.002)	3105525,
CATE_HUMAN	45	CGER.\|.RGFF	inferred from experiment	unknown	MEROPS	Takahashi K	Athauda SB et al.:Proteolytic activity and cleava... (A01.010)	1959628,
MMP9_HUMAN	47	ERGF.\|.FFYT	inferred from experiment	unknown	MEROPS	Opdenakker G	Descamps FJ et al.:Gelatinase B is diabetogenic in... (M10.004)	12626433,
CATE_HUMAN	48	RGFF.\|.FYTP	inferred from experiment	unknown	MEROPS	Takahashi K	Athauda SB et al.:Proteolytic activity and cleava... (A01.010)	1959628,
MEP1A_HUMAN	48	RGFF.\|.FYTP	inferred from experiment	unknown	MEROPS	Bond JS	Butler PE et al.:Characterization of meprin, a m... (M12.002)	3105525,
IDE_HUMAN	48	RGFF.\|.FYTP	inferred from experiment	unknown	MEROPS	Tang WJ	Guo Q et al.:Molecular basis for the recogni... (M16.002)	19896952,
CATE_HUMAN	49	GFFY.\|.YTPK	inferred from experiment	unknown	MEROPS	Takahashi K	Athauda SB et al.:Proteolytic activity and cleava... (A01.010)	1959628,
MEP1A_HUMAN	49	GFFY.\|.YTPK	inferred from experiment	unknown	MEROPS	Bond JS	Butler PE et al.:Characterization of meprin, a m... (M12.002)	3105525,
IDE_HUMAN	49	GFFY.\|.YTPK	inferred from experiment	unknown	MEROPS	Tang WJ	Guo Q et al.:Molecular basis for the recogni... (M16.002)	19896952,
CBPE_HUMAN	54	PKTR.\|.RREA	inferred from experiment	unknown	MEROPS	Hutton JC	Goodge KA et al.:Translational regulation of pro... (M14.005)	10966857,
CBPE_HUMAN	55	KTRR.\|.REAE	inferred from experiment	unknown	MEROPS	Hutton JC	Goodge KA et al.:Translational regulation of pro... (M14.005)	10966857,
NEC1_HUMAN	56	TRRE.\|.EAED	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
CBPE_HUMAN	87	SLQK.\|.KRGI	inferred from experiment	unknown	MEROPS	Hutton JC	Goodge KA et al.:Translational regulation of pro... (M14.005)	10966857,
CBPE_HUMAN	88	LQKR.\|.RGIV	inferred from experiment	unknown	MEROPS	Hutton JC	Goodge KA et al.:Translational regulation of pro... (M14.005)	10966857,
NEC1_HUMAN	89	QKRG.\|.GIVE	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
NEC2_HUMAN	89	QKRG.\|.GIVE	inferred from experiment	unknown	MEROPS	Chretien M	Seidah NG et al.:Pro-protein convertases of subt... (S08.072)	7845206,
AMPB_HUMAN	89	QKRG.\|.GIVE	inferred from experiment	unknown	MEROPS		Merops AMPB_HUMAN -> INS_HUMAN @89
AMPB_HUMAN	89	QKRG.\|.GIVE	inferred from experiment	unknown	MEROPS	Authier F	Kouach M et al.:Endosomal proteolysis of intern... (M01.014)	19834685,
MMP9_HUMAN	101	ICSL.\|.LYQL	inferred from experiment	unknown	MEROPS	Opdenakker G	Descamps FJ et al.:Gelatinase B is diabetogenic in... (M10.004)	12626433,
IDE_HUMAN	102	CSLY.\|.YQLE	inferred from experiment	unknown	MEROPS	Tang WJ	Guo Q et al.:Molecular basis for the recogni... (M16.002)	19896952,
IDE_HUMAN	103	SLYQ.\|.QLEN	inferred from experiment	unknown	MEROPS	Tang WJ	Guo Q et al.:Molecular basis for the recogni... (M16.002)	19896952,
MMP9_HUMAN	104	LYQL.\|.LENY	inferred from experiment	unknown	MEROPS	Opdenakker G	Descamps FJ et al.:Gelatinase B is diabetogenic in... (M10.004)	12626433,
MMP9_HUMAN	106	QLEN.\|.NYCN	inferred from experiment	unknown	MEROPS	Opdenakker G	Descamps FJ et al.:Gelatinase B is diabetogenic in... (M10.004)	12626433,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)