P23528: Cofilin-1

General Information

Protein names	- Cofilin-1 - 18 kDa phosphoprotein - p18 - Cofilin, non-muscle isoform
Gene names	CFL1
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P23528

20 N-termini

17 C-termini

21 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV
        70         80         90        100        110        120
GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS
       130        140        150        160
KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL

Isoforms

Sequence View

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Protease Assignment Confidence:

Evidence Names:

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Protein Neighborhood

Domains & Features

20 N-termini - 17 C-termini - 21 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Modification	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P23528-2-unknown	MASGVA...	1		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P23528-2-unknown	MASGVA...	1			unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P23528-2-Acetylation	ASGVAV...	2	acetylation-	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P23528-2-Acetylation	ASGVAV...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: Complementary positional proteomics for screening substrates...	20526345
P23528-2-Acetylation	ASGVAV...	2	acetylation-		other		CNRS/ISV	Large_scale_NTA_Human
P23528-2-Acetylation	ASGVAV...	2	acetylation-		COFRADIC		Gevaert K.	Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates...	20526345
P23528-2-Acetylation	ASGVAV...	2	acetylation-		N-TAILS		Overall Laboratory	Lange_Huesgen_et_al-RBC
P23528-5-unknown	VAVSDG...	5		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3723
P23528-9-unknown	DGVIKV...	9		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17516
P23528-9-unknown	DGVIKV...	9		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18940
P23528-9-unknown	DGVIKV...	9		inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P23528-9-unknown	DGVIKV...	9		inferred from similarity	unknown	UniProtKB		inferred from uniprot (by similarity)
P23528-10-unknown	GVIKVF...	10		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18941
P23528-18-unknown	DMKVRK...	17		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000432155
P23528-18-unknown	DMKVRK...	17		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000433910
P23528-18-unknown	DMKVRK...	17		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000432226
P23528-18-unknown	DMKVRK...	17		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000433131
P23528-18-unknown	DMKVRK...	17		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000436899
P23528-18-unknown	DMKVRK...	17		inferred from electronic annotation	unknown	Ensembl		inferred from ensembl protein ENSP00000433308
P23528-18-unknown	DMKVRK...	17		inferred from electronic annotation	unknown	TISdb		inferred from TISdb
P23528-18-unknown	DMKVRK...	17		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC2547
P23528-22-	KSSTPE...	22			Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P23528-22-	KSSTPE...	22			Subtiligase Based Positive Selection		Wells	apoptotic_DB_Doxo	23264352
P23528-23-unknown	SSTPEE...	23			unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P23528-23-	SSTPEE...	23			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P23528-23-	SSTPEE...	23			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P23528-46-	NIILEE...	46			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P23528-46-	NIILEE...	46			Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P23528-59-unknown	DVGQTV...	59		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17517
P23528-59-unknown	DVGQTV...	59		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3724
P23528-59-unknown	DVGQTV...	59		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18942
P23528-62-unknown	QTVDDP...	62		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7382
P23528-70-unknown	TFVKML...	70		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17518
P23528-70-unknown	TFVKML...	70		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18943
P23528-83-	ALYDAT...	83			Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P23528-104-unknown	WAPESA...	104		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC7383
P23528-129-unknown	TGIKHE...	129		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17519
P23528-134-unknown	ELQANC...	134		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18944
P23528-134-unknown	ELQANC...	134		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17520
P23528-135-unknown	LQANCY...	135		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18945
P23528-135-unknown	LQANCY...	135		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17521
P23528-137-unknown	ANCYEE...	137		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC18946
P23528-137-unknown	ANCYEE...	137		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5716
P23528-137-unknown	ANCYEE...	137		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17522
P23528-157-unknown	AVISLE...	157		inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17523

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...GVAVSD	8	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17516
...GVAVSD	8	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18940
...VAVSDG	9	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18941
...KVFNDM	17	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC2547
...EILVGD	58	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17517
...EILVGD	58	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3724
...EILVGD	58	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18942
...VGDVGQ	61	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7382
...DDPYAT	69	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17518
...DDPYAT	69	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18943
...DATYET	90	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000432153
...KESKKE	96	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000433308
...LVFIFW	103	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC7383
...IKKKLT	128	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17519
...TGIKHE	133	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18944
...TGIKHE	133	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17520
...GIKHEL	134	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18945
...GIKHEL	134	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17521
...KHELQA	136	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC18946
...KHELQA	136	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5716
...KHELQA	136	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17522
...LQANCY	139	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000436899
...KLGGSA	156	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17523
...GSAVIS	159	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000436431
...EGKPL	166	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CATB_HUMAN	4	ASGV.\|.VAVS	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1A_HUMAN	8	AVSD.\|.DGVI	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1B_HUMAN	8	AVSD.\|.DGVI	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1B_HUMAN	9	VSDG.\|.GVIK	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CASP6_HUMAN	17	FNDM.\|.MKVR	inferred from experiment	unknown	MEROPS	Leblanc AC	Klaiman G et al.:Targets of caspase-6 activity i... (C14.005)	18487604,
MEP1A_HUMAN	58	LVGD.\|.DVGQ	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATB_HUMAN	58	LVGD.\|.DVGQ	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1B_HUMAN	58	LVGD.\|.DVGQ	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATS_HUMAN	61	DVGQ.\|.QTVD	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1A_HUMAN	69	PYAT.\|.TFVK	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1B_HUMAN	69	PYAT.\|.TFVK	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATS_HUMAN	103	FIFW.\|.WAPE	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1A_HUMAN	128	KKLT.\|.TGIK	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1B_HUMAN	133	IKHE.\|.ELQA	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1A_HUMAN	133	IKHE.\|.ELQA	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1B_HUMAN	134	KHEL.\|.LQAN	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1A_HUMAN	134	KHEL.\|.LQAN	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1B_HUMAN	136	ELQA.\|.ANCY	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATL1_HUMAN	136	ELQA.\|.ANCY	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
MEP1A_HUMAN	136	ELQA.\|.ANCY	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
MEP1A_HUMAN	156	GGSA.\|.AVIS	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)