P23588: Eukaryotic translation initiation factor 4B

General Information

Protein names	- Eukaryotic translation initiation factor 4B - eIF-4B
Gene names	EIF4B
Organism	Homo sapiens
Protease Family
Protease ID
Chromosome location
UniProt ID	P23588

20 N-termini

11 C-termini

8 Cleavages

0 Substrates

Sequence

        10         20         30         40         50         60
MAASAKKKNK KGKTISLTDF LAEDGGTGGG STYVSKPVSW ADETDDLEGD VSTTWHSNDD
        70         80         90        100        110        120
DVYRAPPIDR SILPTAPRAA REPNIDRSRL PKSPPYTAFL GNLPYDVTEE SIKEFFRGLN
       130        140        150        160        170        180
ISAVRLPREP SNPERLKGFG YAEFEDLDSL LSALSLNEES LGNRRIRVDV ADQAQDKDRD
       190        200        210        220        230        240
DRSFGRDRNR DSDKTDTDWR ARPATDSFDD YPPRRGDDSF GDKYRDRYDS DRYRDGYRDG
       250        260        270        280        290        300
YRDGPRRDMD RYGGRDRYDD RGSRDYDRGY DSRIGSGRRA FGSGYRRDDD YRGGGDRYED
       310        320        330        340        350        360
RYDRRDDRSW SSRDDYSRDD YRRDDRGPPQ RPKLNLKPRS TPKEDDSSAS TSQSTRAASI
       370        380        390        400        410        420
FGGAKPVDTA AREREVEERL QKEQEKLQRQ LDEPKLERRP RERHPSWRSE ETQERERSRT
       430        440        450        460        470        480
GSESSQTGTS TTSSRNARRR ESEKSLENET LNKEEDCHSP TSKPPKPDQP LKVMPAPPPK
       490        500        510        520        530        540
ENAWVKRSSN PPARSQSSDT EQQSPTSGGG KVAPAQPSEE GPGRKDENKV DGMNAPKGQT
       550        560        570        580        590        600
GNSSRGPGDG GNRDHWKESD RKDGKKDQDS RSAPEPKKPE ENPASKFSSA SKYAALSVDG
       610
EDENEGEDYA E

Isoforms

- Isoform 2 of Eukaryotic translation initiation factor 4B

Sequence View

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Protein Neighborhood

Domains & Features

20 N-termini - 11 C-termini - 8 Cleavages - 0 Substrates

N-termini

Name	Sequence	Position	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMID)
P23588-1-unknown	MAASAK...	1	inferred from electronic annotation	electronic annotation	UniProtKB		inferred from uniprot
P23588-46-unknown	DLEGDV...	46	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1738
P23588-49-	GDVSTT...	49		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P23588-49-	GDVSTT...	49		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P23588-51-	VSTTWH...	51		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P23588-51-	VSTTWH...	51		Subtiligase Based Positive Selection		Wells	apoptotic_RPMI_Dox	23264352
P23588-64-unknown	RAPPID...	64		unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P23588-207-	SFDDYP...	207		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P23588-207-	SFDDYP...	207		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P23588-207-unknown	SFDDYP...	207	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC11349
P23588-340-	STPKED...	340		Subtiligase Based Positive Selection		Wells	DB_Untreated	23264352
P23588-340-	STPKED...	340		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P23588-340-unknown	STPKED...	340	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC10039
P23588-357-unknown	AASIFG...	357		unknown			Mahrus S. et al.: nonapototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P23588-357-unknown	AASIFG...	357		unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P23588-357-	AASIFG...	357		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P23588-357-	AASIFG...	357		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P23588-357-	AASIFG...	357		Subtiligase Based Positive Selection		Wells	Jurkat_untreated	23264352
P23588-357-	AASIFG...	357		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P23588-357-	AASIFG...	357		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P23588-357-	AASIFG...	357		Subtiligase Based Positive Selection		Wells	MM1SDBJurkat_Untreated	23264352
P23588-357-	AASIFG...	357		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P23588-357-	AASIFG...	357		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P23588-359-unknown	SIFGGA...	359	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC17524
P23588-359-	SIFGGA...	359		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P23588-359-	SIFGGA...	359		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P23588-359-	SIFGGA...	359		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P23588-359-	SIFGGA...	359		Subtiligase Based Positive Selection		Wells	apoptotic_MM1SDBJurkat_Mix	23264352
P23588-359-	SIFGGA...	359		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P23588-362-	GGAKPV...	362		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_Etoposide	23264352
P23588-362-unknown	GGAKPV...	362		unknown			Mahrus S. et al.: apototic Jurkat cells, Global Sequencing of Proteolytic Cleavage...	18722006
P23588-363-unknown	GAKPVD...	363	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC5717
P23588-473-	VMPAPP...	473		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_bortezomib	23264352
P23588-473-	VMPAPP...	473		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P23588-473-	VMPAPP...	473		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P23588-532-	GMNAPK...	532		Subtiligase Based Positive Selection		Wells	apoptotic_Jurkat_staurotrail	23264352
P23588-532-	GMNAPK...	532		Subtiligase Based Positive Selection		Wells	apoptotic_MM1s_bort	23264352
P23588-532-	GMNAPK...	532		Subtiligase Based Positive Selection		Wells	apoptosis_U266_bortezomib_induced	23264352
P23588-532-unknown	GMNAPK...	532	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC3220
P23588-532-unknown	GMNAPK...	532	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC1739
P23588-534-unknown	NAPKGQ...	534	inferred from cleavage	unknown	TopFIND		Inferred from cleavage TC14043

C-termini

Sequence	Position	Evidence type	Method	Source (database)	Evidence name
...ADETDD	45	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC1738
...RPATDS	206	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC11349
...YPPRRG	215	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000447192
...SRDDYR	321	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000450324
...NLKPRS	339	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC10039
...STRAAS	358	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC17524
...ASIFGG	362	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC5717
...RERHPS	405	inferred from electronic annotation	unknown	Ensembl	inferred from ensembl protein ENSP00000449746
...ENKVDG	531	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC3220
...ENKVDG	531	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC1739
...KVDGMN	533	inferred from cleavage	unknown	TopFIND	Inferred from cleavage TC14043
...EDYAE	611	inferred from electronic annotation	electronic annotation	UniProtKB	inferred from uniprot

Cleavages

Protease	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)
CASP3_HUMAN	45	ETDD.\|.DLEG	inferred from experiment	unknown	MEROPS	Morley SJ	Bushell M et al.:Changes in integrity and associ... (C14.003)	10672017,
GRAB_HUMAN	206	ATDS.\|.SFDD	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:Complementary positional proteo... (M14.017)	20526345,
GRAA_HUMAN	339	KPRS.\|.STPK	inferred from experiment	unknown	MEROPS	Gevaert K	Van Damme P et al.:The substrate specificity profi... (S01.135)	20536382,
MEP1A_HUMAN	358	RAAS.\|.SIFG	inferred from experiment	unknown	MEROPS	Overall CM	Becker-Pauly C et al.:Proteomic analyses reveal an ac... (M12.002)	21693781,
CATL1_HUMAN	362	IFGG.\|.GAKP	inferred from experiment	unknown	MEROPS	Schilling O	Biniossek ML et al.:Proteomic identification of pro... (C01.060)	21967108,
CASPE_HUMAN	531	KVDG.\|.GMNA	inferred from experiment	unknown	MEROPS		Merops CASPE_HUMAN -> IF4B_HUMAN @531
CASP3_HUMAN	531	KVDG.\|.GMNA	inferred from experiment	unknown	MEROPS		Merops CASP3_HUMAN -> IF4B_HUMAN @531
GRAM_HUMAN	533	DGMN.\|.NAPK	inferred from experiment	unknown	MEROPS	Bovenschen N	de Poot SA et al.:Human and mouse granzyme M disp... (S01.139)	21564021,

Substrates

Substrate	Position	Sequence	Evidence type	Method	Source (database)	Source (Lab)	Evidence name	Publications (PMIDs)